Reviewed, UniProtKB/Swiss-Prot Q6GQK9 (NDST1_XENLA)

Names and origin

Protein names

Recommended name:
    Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1
    EC=2.8.2.8
Alternative name(s):
    Glucosaminyl N-deacetylase/N-sulfotransferase 1
      Short name=NDST-1
Including the following 2 domains:
    1- Recommended name:
            Heparan sulfate N-deacetylase 1
              EC=3.-.-.-
    2- Recommended name:
            Heparan sulfate N-sulfotransferase 1
              EC=2.8.2.-

Gene names

Name:

ndst1

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	878 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA dissacharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis By similarity.
Catalytic activity	3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine.
Pathway	Glycan metabolism; heparan sulfate biosynthesis. Glycan metabolism; heparin biosynthesis.
Subunit structure	Monomer By similarity.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the sulfotransferase 1 family. NDST subfamily.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Hydrolase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	[heparan sulfate]-glucosamine N-sulfotransferase activity Inferred from electronic annotation. Source: EC hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 878	878	Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1		PRO_0000225656
Regions
	Topological domain	1 – 17	17	Cytoplasmic Potential
	Transmembrane	18 – 38	21	Signal-anchor for type II membrane protein Potential
	Topological domain	39 – 878	840	Lumenal Potential
	Nucleotide binding	610 – 614	5	PAPS By similarity
	Nucleotide binding	829 – 833	5	PAPS By similarity
	Region	40 – 594	555	Heparan sulfate N-deacetylase 1
	Region	595 – 878	284	Heparan sulfate N-sulfotransferase 1
Sites
	Active site	610	1	For sulfotransferase activity By similarity
	Binding site	708	1	PAPS By similarity
Amino acid modifications
	Glycosylation	231	1	N-linked (GlcNAc...) Potential
	Glycosylation	347	1	N-linked (GlcNAc...) Potential
	Glycosylation	397	1	N-linked (GlcNAc...) Potential
	Glycosylation	663	1	N-linked (GlcNAc...) Potential
	Disulfide bond	814 ↔ 824		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6GQK9-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 6FC6721BDAF3B17E	FASTA	878	101,119
	10 20 30 40 50 60 MSLSLKTRRF GRPVRPQLVL LLLFALCLLS VFISAYYLYG WKRGLEPSGS EAQSPDCDEP 70 80 90 100 110 120 KISPSRLLPV KPLKPVDSSR TDPLVLVFVE SLYSQLGQEI VAILESSRFK YRTEIAPGKG 130 140 150 160 170 180 DMPTLTDKDR GRFALIVYEN ILKYVNLDAW NRELLDKYCV EYGVGIIGFF KANENSLLSA 190 200 210 220 230 240 QLKGFPLYLH SNLGLKDCSI NPKSPLLYIT RPNQVEKGDL PGEDWTVFQS NHSTYEPVLL 250 260 270 280 290 300 AKTKSAESIP HLSVDAALHT TVVQDLGLHD GIQRVLFGNN LNFWLHKLVF VDAVSFLTGK 310 320 330 340 350 360 RLSLPLNRYV LVDIDDIFVG KEGTRMKVED VKALYDTQME LRTHIPNFTF NLGFSGKFFH 370 380 390 400 410 420 TGTDAEDEGD DLLLSYVKQF WWFPHMWSHM QPHLFHNQSV LAEQMALNRK FAVDHGIPTD 430 440 450 460 470 480 MGYAVAPHHS GVYPVHVQLY EAWKQIWGIK VTSTEEYPHL KPARYRRGFV HNGIMILPRQ 490 500 510 520 530 540 TCGLFTHTIF YNEYPGGPVE LDKIINGGEL FLTVLLNPIS IFMTHLSNYG NDRLGLYTFK 550 560 570 580 590 600 LLVQFLNTWT NLKLETLPPV QLAHKYFQIF PEEKDPLWQD PCEDKRHKDI WSKEKTCDRF 610 620 630 640 650 660 PKLLIIGPQK TGTTALYLFL GMHSDLSSNY PSSETFEEIQ FYNGQNYHKG IDWYMEFFPI 670 680 690 700 710 720 PSNTTSDFYF EKSANYFDSE LAPRRVAALL PKAKIITILI NPADRAYSWY QHQRAHDDPV 730 740 750 760 770 780 AIKYTFQEVI KAGPEAPQRL RALQNRCLVP GWYSTHIERW MNHFHANQIL VLDGKLLRTE 790 800 810 820 830 840 PANVMETVQK FLGVTNAMDY HKTLAFDPKK GFWCQLLDGG KTKCLGKSKG RKYPDMDSDS 850 860 870 RSFLMDYYRD HNIELSKLLY KMGQTLPTWL REELQNTR

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Spleen.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC072733 mRNA. Translation: AAH72733.1.
RefSeq	NP_001085429.1.
UniGene	Xl.78515
3D structure databases
SMR	Q6GQK9. Positions 588-875.
ModBase	Search...
Genome annotation databases
GeneID	443855.
KEGG	xla:443855.
Organism-specific databases
Xenbase	XB-FEAT-955051. ndst1.
Phylogenomic databases
HOVERGEN	Q6GQK9.
Enzyme and pathway databases
BRENDA	2.8.2.8. 648.
Family and domain databases
InterPro	IPR000863. Sulfotransferase. [Graphical view]
Pfam	PF00685. Sulfotransfer_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDST1_XENLA

Accession

Primary (citable) accession number: Q6GQK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	July 19, 2004
Last modified:	October 13, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project