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Protein

E3 ubiquitin-protein ligase rnf146

Gene

rnf146

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated proteins and mediates their ubiquitination and subsequent degradation. May regulate many important biological processes, such as cell survival and DNA damage response. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of poly-ADP-ribosylated proteins. Neuroprotective protein. Protects against cell death induced by DNA damaging agents and rescues cells from G1 arrest. Promotes cell survival after gamma-irradiation. Facilitates DNA repair.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei107iso-ADP-ribose adenine ring groupBy similarity1
Binding sitei110iso-ADP-ribose 5'-phosphate groupBy similarity1
Binding sitei114iso-ADP-ribose 5'-phosphate groupBy similarity1
Binding sitei144iso-ADP-ribose 1'-phosphate groupBy similarity1
Binding sitei153iso-ADP-ribose adenine ring groupBy similarity1
Binding sitei163iso-ADP-ribose 1'-phosphate groupBy similarity1
Binding sitei175iso-ADP-ribose 5'-phosphate groupBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri36 – 74RING-typePROSITE-ProRule annotationAdd BLAST39

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway, Wnt signaling pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase rnf146 (EC:2.3.2.27)
Alternative name(s):
RING finger protein 146
RING-type E3 ubiquitin transferase rnf146Curated
Gene namesi
Name:rnf146
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-974784. rnf146.

Subcellular locationi

  • Cytoplasmcytosol By similarity
  • Nucleus By similarity

  • Note: Translocates to the nucleus after DNA damage.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004095071 – 314E3 ubiquitin-protein ligase rnf146Add BLAST314

Interactioni

Protein-protein interaction databases

BioGridi102058. 1 interactor.
IntActiQ6GQD5. 1 interactor.

Structurei

3D structure databases

ProteinModelPortaliQ6GQD5.
SMRiQ6GQD5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini91 – 167WWEPROSITE-ProRule annotationAdd BLAST77

Domaini

The WWE domain mediates non-covalent poly(ADP-ribose)-binding.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri36 – 74RING-typePROSITE-ProRule annotationAdd BLAST39

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOVERGENiHBG057514.
KOiK15700.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR033509. RNF146.
IPR004170. WWE-dom.
IPR018123. WWE-dom_subgr.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
PANTHERiPTHR13417:SF8. PTHR13417:SF8. 1 hit.
PfamiView protein in Pfam
PF02825. WWE. 1 hit.
SMARTiView protein in SMART
SM00184. RING. 1 hit.
SM00678. WWE. 1 hit.
PROSITEiView protein in PROSITE
PS50918. WWE. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6GQD5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGCGEVSHS ANMLPTNKKL DEPCSNNAPS LSVPECAICL QICVHPVSLP
60 70 80 90 100
CKHIFCYLCV KGASWLGRRC ALCRQEIPED FLDKPTLLSP EDLKSASRGN
110 120 130 140 150
GEYAWYYEGR NGWWQYDERT SRELEDAFTK GKKSTEMLIA GFLYVADLEN
160 170 180 190 200
MVQYRRNEHG RRRKIKRDIV DIPKKGVAGL RLECDAANLN LARESSADGA
210 220 230 240 250
DSMAALGDSS SQPAHVLPAR LHTSLSTTTS QVLSHSDVTT SLENSFTQLQ
260 270 280 290 300
IGDPVINRNY IGEEGRPLII ARMQATNAVL EEQEPSDRND HGSPALQHNS
310
FLVPQSNRLP FGNP
Length:314
Mass (Da):34,771
Last modified:July 19, 2004 - v1
Checksum:i3FAF256C14E0F227
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC072810 mRNA. Translation: AAH72810.1.
RefSeqiNP_001085469.1. NM_001092000.1.
UniGeneiXl.14626.

Genome annotation databases

GeneIDi443895.
KEGGixla:443895.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC072810 mRNA. Translation: AAH72810.1.
RefSeqiNP_001085469.1. NM_001092000.1.
UniGeneiXl.14626.

3D structure databases

ProteinModelPortaliQ6GQD5.
SMRiQ6GQD5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi102058. 1 interactor.
IntActiQ6GQD5. 1 interactor.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi443895.
KEGGixla:443895.

Organism-specific databases

CTDi443895.
XenbaseiXB-GENE-974784. rnf146.

Phylogenomic databases

HOVERGENiHBG057514.
KOiK15700.

Enzyme and pathway databases

UniPathwayiUPA00143.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR033509. RNF146.
IPR004170. WWE-dom.
IPR018123. WWE-dom_subgr.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
PANTHERiPTHR13417:SF8. PTHR13417:SF8. 1 hit.
PfamiView protein in Pfam
PF02825. WWE. 1 hit.
SMARTiView protein in SMART
SM00184. RING. 1 hit.
SM00678. WWE. 1 hit.
PROSITEiView protein in PROSITE
PS50918. WWE. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRN146_XENLA
AccessioniPrimary (citable) accession number: Q6GQD5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: July 19, 2004
Last modified: March 15, 2017
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.