Q6GQB9 (EDEM3_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ER degradation-enhancing alpha-mannosidase-like protein 3
EC=3.2.1.113

Alternative name(s):
Alpha-1,2-mannosidase EDEM3

Gene names

Name:

edem3

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	913 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be involved in endoplasmic reticulum-associated degradation (ERAD) By similarity.
Catalytic activity	Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.
Subcellular location	Endoplasmic reticulum lumen By similarity.
Domain	Contains a protease-associated domain (PA) of unknown function By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 47 family. Contains 1 PA (protease associated) domain.
Sequence caution	The sequence AAH72826.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Unfolded protein response
Cellular component	Endoplasmic reticulum
Domain	Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological_process	response to unfolded protein Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15	Potential
	Chain	16 – 913	898	ER degradation-enhancing alpha-mannosidase-like protein 3		PRO_0000364227
Regions
	Domain	660 – 766	107	PA
	Motif	910 – 913	4	Prevents secretion from ER Potential
Sites
	Active site	132	1	Proton donor By similarity
	Active site	279	1	By similarity
	Active site	391	1	By similarity
Amino acid modifications
	Glycosylation	104	1	N-linked (GlcNAc...) Potential
	Glycosylation	181	1	N-linked (GlcNAc...) Potential
	Glycosylation	497	1	N-linked (GlcNAc...) Potential
	Glycosylation	797	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6GQB9 [UniParc]. Last modified March 3, 2009. Version 2. Checksum: BB7164FF7FA2BC83	FASTA	913	102,735
	10 20 30 40 50 60 MGCPAVEARR WGDMWLVVAF CLLGHGHAAV TKEEKAHLRS QVLEMFDHAY GNYMQHAYPA 70 80 90 100 110 120 DELMPLTCRG RIRGQEPSRG DVDDALGKFS LTLIDTLDTL VVLNKTKEFE DAVRKVITDV 130 140 150 160 170 180 NLDNDIVVSV FETNIRVLGG LLGGHSVAIM LKENGDGMQW YNDELLHMAK ELGYKLLPAF 190 200 210 220 230 240 NTTSGLPYPR INLKFGIRRP EARTGTETDT CTACAGTMIL EFAALSRFTG ISVFEEHARK 250 260 270 280 290 300 ALDFLWDKRQ RSSNLVGVTI NIHTGDWVRK DSGVGAGIDS YYEYLLKAYV LLGDDSYLER 310 320 330 340 350 360 FNTHYDAIMR YISQPPLLLD VHIHKPMLTA RTWMDSLLAF FPGLQVLKGD IRPAIETHEM 370 380 390 400 410 420 LYQVIKKHNF LPEAFTTDFR VHWAQHPLRP EFAESTYFLY KATGDPYYLE VGKTLIDNLN 430 440 450 460 470 480 KYARVPCGFA AVKDVRTGSH EDRMDSFFLA EMFKYLYLLF SEREDLIFDI EDYIFTTEAH 490 500 510 520 530 540 LLPLSLSTAN PSSTKKNTTT QYTELDDSNF DWSCPNTQIL FRNDPMYAQN IREPLKNVVD 550 560 570 580 590 600 KNCPRSPSRL DEISGSGKMP PLRARDFMAS NSEHLEILKK MGVSLIHLKD GRVQLVQHAN 610 620 630 640 650 660 QAASSIDAED GLRFMQEMIE LSSQQQKEQQ LPPRAVQIVS HPFYGRVVLT AGPAQFGMDL 670 680 690 700 710 720 SKHLAGAQGL VARAEPYSGC SDITNGQAIQ GKIALMQRGQ CMFAEKARNV QKAGAIGGIV 730 740 750 760 770 780 IDDNEGSSSD TAPLFQMAGD GKSTDDVTIP MLFLFSKEGN IILDAIREYQ QVEVLLSDKA 790 800 810 820 830 840 KDRDLESESG EQKPVENDSQ KQALEDLFMT PEEIAELLIV HEEESPVSQP EVPSSDSPSG 850 860 870 880 890 900 GDRTSERDIT PESQEHKTEE TEHSPKDNVQ TPPENSEDST EEKMDNKVQP MESILADWKE 910 DIEAFEMMEK DEL

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC072826 mRNA. Translation: AAH72826.1. Different initiation.
RefSeq	NP_001085481.2. NM_001092012.1.
UniGene	Xl.47665.
3D structure databases
HSSP	HSSP built from PDB template 1FO3 based on UniProtKB Q9UKM7.
ProteinModelPortal	Q6GQB9.
ModBase	Search...
Protein family/group databases
CAZy	GH47. Glycoside Hydrolase Family 47.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	443907.
KEGG	xla:443907.
Organism-specific databases
CTD	80267.
Xenbase	XB-GENE-5811512. edem3.
Phylogenomic databases
HOVERGEN	HBG107835.
KO	K10086.
Family and domain databases
Gene3D	1.50.10.50. 1 hit.
InterPro	IPR001382. Glyco_hydro_47. IPR003137. Protease-assoc_domain. [Graphical view]
PANTHER	PTHR11742. PTHR11742. 1 hit.
Pfam	PF01532. Glyco_hydro_47. 1 hit. PF02225. PA. 1 hit. [Graphical view]
PRINTS	PR00747. GLYHDRLASE47.
SUPFAM	SSF48225. Glyco_hydro_47. 1 hit.
PROSITE	PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EDEM3_XENLA

Accession

Primary (citable) accession number: Q6GQB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	March 6, 2013
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program