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Protein

ER degradation-enhancing alpha-mannosidase-like protein 3

Gene

edem3

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

May be involved in endoplasmic reticulum-associated degradation (ERAD).By similarity

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei132 – 1321Proton donorBy similarity
Active sitei279 – 2791By similarity
Active sitei391 – 3911By similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. response to unfolded protein Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Unfolded protein response

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

Names & Taxonomyi

Protein namesi
Recommended name:
ER degradation-enhancing alpha-mannosidase-like protein 3 (EC:3.2.1.113)
Alternative name(s):
Alpha-1,2-mannosidase EDEM3
Gene namesi
Name:edem3
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-5811512. edem3.

Subcellular locationi

Endoplasmic reticulum lumen PROSITE-ProRule annotation

GO - Cellular componenti

  1. endoplasmic reticulum lumen Source: UniProtKB-SubCell
  2. membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1515Sequence AnalysisAdd
BLAST
Chaini16 – 913898ER degradation-enhancing alpha-mannosidase-like protein 3PRO_0000364227Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi104 – 1041N-linked (GlcNAc...)Sequence Analysis
Glycosylationi181 – 1811N-linked (GlcNAc...)Sequence Analysis
Glycosylationi497 – 4971N-linked (GlcNAc...)Sequence Analysis
Glycosylationi797 – 7971N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ6GQB9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini660 – 766107PAAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi910 – 9134Prevents secretion from ERPROSITE-ProRule annotation

Domaini

Contains a protease-associated domain (PA) of unknown function.By similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG107835.
KOiK10086.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
IPR003137. Protease-assoc_domain.
[Graphical view]
PANTHERiPTHR11742. PTHR11742. 1 hit.
PfamiPF01532. Glyco_hydro_47. 1 hit.
PF02225. PA. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6GQB9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCPAVEARR WGDMWLVVAF CLLGHGHAAV TKEEKAHLRS QVLEMFDHAY
60 70 80 90 100
GNYMQHAYPA DELMPLTCRG RIRGQEPSRG DVDDALGKFS LTLIDTLDTL
110 120 130 140 150
VVLNKTKEFE DAVRKVITDV NLDNDIVVSV FETNIRVLGG LLGGHSVAIM
160 170 180 190 200
LKENGDGMQW YNDELLHMAK ELGYKLLPAF NTTSGLPYPR INLKFGIRRP
210 220 230 240 250
EARTGTETDT CTACAGTMIL EFAALSRFTG ISVFEEHARK ALDFLWDKRQ
260 270 280 290 300
RSSNLVGVTI NIHTGDWVRK DSGVGAGIDS YYEYLLKAYV LLGDDSYLER
310 320 330 340 350
FNTHYDAIMR YISQPPLLLD VHIHKPMLTA RTWMDSLLAF FPGLQVLKGD
360 370 380 390 400
IRPAIETHEM LYQVIKKHNF LPEAFTTDFR VHWAQHPLRP EFAESTYFLY
410 420 430 440 450
KATGDPYYLE VGKTLIDNLN KYARVPCGFA AVKDVRTGSH EDRMDSFFLA
460 470 480 490 500
EMFKYLYLLF SEREDLIFDI EDYIFTTEAH LLPLSLSTAN PSSTKKNTTT
510 520 530 540 550
QYTELDDSNF DWSCPNTQIL FRNDPMYAQN IREPLKNVVD KNCPRSPSRL
560 570 580 590 600
DEISGSGKMP PLRARDFMAS NSEHLEILKK MGVSLIHLKD GRVQLVQHAN
610 620 630 640 650
QAASSIDAED GLRFMQEMIE LSSQQQKEQQ LPPRAVQIVS HPFYGRVVLT
660 670 680 690 700
AGPAQFGMDL SKHLAGAQGL VARAEPYSGC SDITNGQAIQ GKIALMQRGQ
710 720 730 740 750
CMFAEKARNV QKAGAIGGIV IDDNEGSSSD TAPLFQMAGD GKSTDDVTIP
760 770 780 790 800
MLFLFSKEGN IILDAIREYQ QVEVLLSDKA KDRDLESESG EQKPVENDSQ
810 820 830 840 850
KQALEDLFMT PEEIAELLIV HEEESPVSQP EVPSSDSPSG GDRTSERDIT
860 870 880 890 900
PESQEHKTEE TEHSPKDNVQ TPPENSEDST EEKMDNKVQP MESILADWKE
910
DIEAFEMMEK DEL
Length:913
Mass (Da):102,735
Last modified:March 3, 2009 - v2
Checksum:iBB7164FF7FA2BC83
GO

Sequence cautioni

The sequence AAH72826.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC072826 mRNA. Translation: AAH72826.1. Different initiation.
RefSeqiNP_001085481.2. NM_001092012.1.
UniGeneiXl.47665.

Genome annotation databases

GeneIDi443907.
KEGGixla:443907.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC072826 mRNA. Translation: AAH72826.1. Different initiation.
RefSeqiNP_001085481.2. NM_001092012.1.
UniGeneiXl.47665.

3D structure databases

ProteinModelPortaliQ6GQB9.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi443907.
KEGGixla:443907.

Organism-specific databases

CTDi80267.
XenbaseiXB-GENE-5811512. edem3.

Phylogenomic databases

HOVERGENiHBG107835.
KOiK10086.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
IPR003137. Protease-assoc_domain.
[Graphical view]
PANTHERiPTHR11742. PTHR11742. 1 hit.
PfamiPF01532. Glyco_hydro_47. 1 hit.
PF02225. PA. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo.

Entry informationi

Entry nameiEDEM3_XENLA
AccessioniPrimary (citable) accession number: Q6GQB9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: March 3, 2009
Last modified: January 7, 2015
This is version 56 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.