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Protein

EGF domain-specific O-linked N-acetylglucosamine transferase

Gene

eogt

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in extracellular proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Specifically glycosylates the Thr residue located between the fifth and sixth conserved cysteines of folded EGF-like domains (By similarity).By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + [protein]-L-serine = UDP + [protein]-3-O-(N-acetyl-D-glucosaminyl)-L-serine.
UDP-N-acetyl-D-glucosamine + [protein]-L-threonine = UDP + [protein]-3-O-(N-acetyl-D-glucosaminyl)-L-threonine.

GO - Molecular functioni

  1. protein N-acetylglucosaminyltransferase activity Source: UniProtKB

GO - Biological processi

  1. protein O-linked glycosylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Protein family/group databases

CAZyiGT61. Glycosyltransferase Family 61.

Names & Taxonomyi

Protein namesi
Recommended name:
EGF domain-specific O-linked N-acetylglucosamine transferase (EC:2.4.1.255)
Alternative name(s):
Extracellular O-linked N-acetylglucosamine transferase
Gene namesi
Name:eogt
Synonyms:aer61
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-994622. eogt.

Subcellular locationi

Endoplasmic reticulum lumen PROSITE-ProRule annotation

GO - Cellular componenti

  1. endoplasmic reticulum lumen Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 525501EGF domain-specific O-linked N-acetylglucosamine transferasePRO_0000301976Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi352 – 3521N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi293 – 2953Required for optimal activityBy similarity
Motifi522 – 5254Prevents secretion from ERPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the glycosyltransferase 61 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG056678.
KOiK18134.

Family and domain databases

InterProiIPR007657. Glycosyltransferase_AER61.
[Graphical view]
PfamiPF04577. DUF563. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6GQ23-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVPLRLVLLL HIIHFSCENE VGSAANNGSA QLYNYRKIHL PDDHIPYYLH
60 70 80 90 100
SNRHVAALCL QDLHCPYKQH LQNLNSCWGY EKTCAEGHRF GYPVCDQVDF
110 120 130 140 150
GWAKTIEESQ QVFWRQADFG YVKERLAETQ ILCRPQEQGD SMLACSQNLQ
160 170 180 190 200
HCRATNLYLD LRHPRRGQEN FKEDFLQEGE IGGHCDLDKQ ALLSQGAWKS
210 220 230 240 250
PLQSWFAELQ SYSSFKFKPI EDAHCDIIIE KPTYFMKLDA GVNMYHHFCD
260 270 280 290 300
FVNLYITQHV NNSFSTDINI VMWTTSVYGY GDLFSDTWKA FTDYEITHLK
310 320 330 340 350
AYDNKRVCFK DAVFALLPRM RYGLFYNTPL ISHCHGSGLF RAFSQHVLHR
360 370 380 390 400
LNITQHPATE AKIRVTILVR STEFRKILNL DELVQALEAV PTFQVKVVDY
410 420 430 440 450
KYRVLGFLEQ LSITHNSDIF IGMHGAGLTH LLFLPDWAVV FELYNCEDAR
460 470 480 490 500
CYLDLARLRG IQYMTWEKGD KVFPQDKGHH PNLGEHPKFT NYAFDVEEFL
510 520
RLVQQGATYV SRHSKWPLRR TRDEL
Length:525
Mass (Da):61,182
Last modified:July 19, 2004 - v1
Checksum:i71165EA2755AA496
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC072925 mRNA. Translation: AAH72925.1.
RefSeqiNP_001085557.1. NM_001092088.1.
UniGeneiXl.7160.

Genome annotation databases

GeneIDi443983.
KEGGixla:443983.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC072925 mRNA. Translation: AAH72925.1.
RefSeqiNP_001085557.1. NM_001092088.1.
UniGeneiXl.7160.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT61. Glycosyltransferase Family 61.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi443983.
KEGGixla:443983.

Organism-specific databases

CTDi285203.
XenbaseiXB-GENE-994622. eogt.

Phylogenomic databases

HOVERGENiHBG056678.
KOiK18134.

Family and domain databases

InterProiIPR007657. Glycosyltransferase_AER61.
[Graphical view]
PfamiPF04577. DUF563. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Gastrula.

Entry informationi

Entry nameiEOGT_XENLA
AccessioniPrimary (citable) accession number: Q6GQ23
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 19, 2004
Last modified: January 7, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.