Reviewed, UniProtKB/Swiss-Prot Q6GPI1 (CTRB2_HUMAN)

Names and origin

Protein names

Recommended name:
    Chymotrypsinogen B2
    EC=3.4.21.1
Cleaved into the following 3 chains:
    1- Recommended name:
            Chymotrypsin B2 chain A
    2- Recommended name:
            Chymotrypsin B2 chain B
    3- Recommended name:
            Chymotrypsin B2 chain C

Gene names

Name:

CTRB2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	By similarity
	Chain	19 – 263	245	Chymotrypsinogen B2		PRO_0000285870
	Chain	19 – 31	13	Chymotrypsin B2 chain A		PRO_0000285871
	Chain	34 – 164	131	Chymotrypsin B2 chain B		PRO_0000285872
	Chain	167 – 263	97	Chymotrypsin B2 chain C		PRO_0000285873
Regions
	Domain	34 – 261	228	Peptidase S1
Sites
	Active site	75	1	Charge relay system By similarity
	Active site	120	1	Charge relay system By similarity
	Active site	213	1	Charge relay system By similarity
Amino acid modifications
	Disulfide bond	19 ↔ 140		By similarity
	Disulfide bond	60 ↔ 76		By similarity
	Disulfide bond	154 ↔ 219		By similarity
	Disulfide bond	186 ↔ 200		By similarity
	Disulfide bond	209 ↔ 238		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6GPI1-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 9AA816B2898728B7	FASTA	263	27,796
	10 20 30 40 50 60 MASLWLLSCF SLVGAAFGCG VPAIHPVLSG LSRIVNGEDA VPGSWPWQVS LQDKTGFHFC 70 80 90 100 110 120 GGSLISEDWV VTAAHCGVRT SDVVVAGEFD QGSDEENIQV LKIAKVFKNP KFSILTVNND 130 140 150 160 170 180 ITLLKLATPA RFSQTVSAVC LPSADDDFPA GTLCATTGWG KTKYNANKTP DKLQQAALPL 190 200 210 220 230 240 LSNAECKKSW GRRITDVMIC AGASGVSSCM GDSGGPLVCQ KDGAWTLVGI VSWGSRTCST 250 260 TTPAVYARVT KLIPWVQKIL AAN

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas.

Web resources

Wikipedia

Chymotrypsin entry

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC073145 mRNA. Translation: AAH73145.1.
IPI	IPI00742763.
UniGene	Hs.632211
3D structure databases
SMR	Q6GPI1. Positions 19-263.
ModBase	Search...
Protein family/group databases
MEROPS	S01.152.
PTM databases
PhosphoSite	Q6GPI1.
Proteomic databases
PRIDE	Q6GPI1.
Genome annotation databases
Ensembl	ENSG00000168925. Homo sapiens. [Contig view]
Organism-specific databases
GeneCards	GC16M073796.
HGNC	HGNC:2522. CTRB2.
PharmGKB	PA27023.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q6GPI1.
Enzyme and pathway databases
BRENDA	3.4.21.1. 247.
Gene expression databases
CleanEx	HS_CTRB2.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CTRB2_HUMAN

Accession

Primary (citable) accession number: Q6GPI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	July 19, 2004
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)