Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA-splicing endonuclease subunit Sen2

Gene

tsen2

Organism
Xenopus laevis (African clawed frog)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body.UniRule annotation

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotation

Keywords - Biological processi

tRNA processingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonuclease subunit Sen2UniRule annotation (EC:4.6.1.16UniRule annotation)
Gene namesi
Name:tsen2Imported
Synonyms:MGC80553Imported
OrganismiXenopus laevis (African clawed frog)Imported
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-5907126. tsen2.

Subcellular locationi

  • Nucleus UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

NucleusUniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ6GPB4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family.UniRule annotation

Phylogenomic databases

HOVERGENiHBG056610.
KOiK15322.

Family and domain databases

Gene3Di3.40.1350.10. 1 hit.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR016589. tRNA_splic_SEN2.
[Graphical view]
PANTHERiPTHR21227:SF0. PTHR21227:SF0. 1 hit.
PfamiPF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
[Graphical view]
PIRSFiPIRSF011789. tRNA_splic_SEN2. 1 hit.
SUPFAMiSSF53032. SSF53032. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6GPB4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKATFHAPK RKRKVYESYE SPFPIPLAHD HSSRDFHICQ AEILNNHVIV
60 70 80 90 100
RSTEDIELLY GKGYFGKGIL SRSRPQHSIA EQQLQSKWKD IKVNLPIISS
110 120 130 140 150
RRYKLHLEWA KNLLEEQGLG TESINKILAD YTREVDISAE GDVVEENPPA
160 170 180 190 200
ESRMDLDPEN GTKSQSSDSN PGKESEGKVF LEGNPEYDPL AKYGPEVLQE
210 220 230 240 250
ERAEEEPDRN KLEKVHCPKH DDFILHCGCK PRRETLQNEL DSPARPHMEY
260 270 280 290 300
LLVEEVQENV DLGPEGTEEN KGARGRGRLV CRRNPFRIFE YLQLSREEAF
310 320 330 340 350
FLVYALGCLT ISYKKEPLTI QKLWEVFCAA QPSFSTKYLA YHHFRSKGWV
360 370 380 390 400
PKVGLKYGTD LLLYRKGPPF YHASYSVIVE LVNDKGEGTP LRPLSWRSLA
410 420 430 440 450
GLHRTTANVS KELLFCYLIK PAGFTDSDFI SPACIHRIKV QELIVSRWVS
460
SRERMDHEEL
Length:460
Mass (Da):52,870
Last modified:July 19, 2004 - v1
Checksum:iEB3B8BEAF3262E10
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC073227 mRNA. Translation: AAH73227.1.
RefSeqiNP_001085705.1. NM_001092236.1.
UniGeneiXl.34894.

Genome annotation databases

GeneIDi444131.
KEGGixla:444131.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC073227 mRNA. Translation: AAH73227.1.
RefSeqiNP_001085705.1. NM_001092236.1.
UniGeneiXl.34894.

3D structure databases

ProteinModelPortaliQ6GPB4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi444131.
KEGGixla:444131.

Organism-specific databases

CTDi80746.
XenbaseiXB-GENE-5907126. tsen2.

Phylogenomic databases

HOVERGENiHBG056610.
KOiK15322.

Family and domain databases

Gene3Di3.40.1350.10. 1 hit.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR016589. tRNA_splic_SEN2.
[Graphical view]
PANTHERiPTHR21227:SF0. PTHR21227:SF0. 1 hit.
PfamiPF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
[Graphical view]
PIRSFiPIRSF011789. tRNA_splic_SEN2. 1 hit.
SUPFAMiSSF53032. SSF53032. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: SpleenImported.

Entry informationi

Entry nameiQ6GPB4_XENLA
AccessioniPrimary (citable) accession number: Q6GPB4
Entry historyi
Integrated into UniProtKB/TrEMBL: July 19, 2004
Last sequence update: July 19, 2004
Last modified: July 22, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.