Q6GP70 (ADA_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenosine deaminase
EC=3.5.4.4

Alternative name(s):
Adenosine aminohydrolase

Gene names

Name:

ada

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. May act as a positive regulator of T-cell coactivation By similarity.
Catalytic activity	Adenosine + H2O = inosine + NH3.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein; Extracellular side By similarity. Cell junction By similarity. Cytoplasmic vesicle lumen By similarity. Cytoplasm By similarity.
Sequence similarities	Belongs to the adenosine and AMP deaminases family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Cell junction Cell membrane Cytoplasm Cytoplasmic vesicle Membrane
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	adenosine catabolic process Inferred from sequence or structural similarity. Source: UniProtKB inosine biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB purine ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasmic membrane-bounded vesicle lumen Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosine deaminase activity Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	Adenosine deaminase		PRO_0000194357
Sites
	Active site	215	1	Proton donor By similarity
	Metal binding	14	1	Zinc; catalytic By similarity
	Metal binding	16	1	Zinc; catalytic By similarity
	Metal binding	212	1	Zinc; catalytic By similarity
	Metal binding	294	1	Zinc; catalytic By similarity
	Binding site	16	1	Substrate By similarity
	Binding site	18	1	Substrate By similarity
	Binding site	183	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	295	1	Substrate By similarity
	Site	236	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6GP70 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: C6119F12592CB059	FASTA	358	40,676
	10 20 30 40 50 60 MESKAFNKPK VELHVHLDGS IKPETIIHFA KKRQIKLPAD TVEGLLEHVS YKEPLSLTEF 70 80 90 100 110 120 LQKFNHYMPA IAGDREAIKR IAYEFVEMKA KEGVIYVEVR YSPHFLANSK VDPIPWGQKE 130 140 150 160 170 180 GDITPDEVVD LVNQGLRKGE KTFNIKARSI LCCMRHMPNW SSEVIELCKK YQNDTVVAID 190 200 210 220 230 240 LAGDESLNCE SYPGHRKAYE EAVKCGIHRT VHAGEVGPPS VVKEAVEVLK AERIGHGYHT 250 260 270 280 290 300 TEDPNLYKEL LENNMHFEVC PWSSYLTSAC HPDFTKHPAT QFRKDKANFS LNTDDPLIFG 310 320 330 340 350 STLDVDYSIA VQHMGFTEDE FKRVNINAAK SSFLPDNEKK ELLYKLYEAY GMILSTGL

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Spleen.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC073271 mRNA. Translation: AAH73271.1.
RefSeq	NP_001085740.1. NM_001092271.1.
UniGene	Xl.80690.
3D structure databases
ProteinModelPortal	Q6GP70.
SMR	Q6GP70. Positions 3-352.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	444167.
KEGG	xla:444167.
Organism-specific databases
CTD	100.
Xenbase	XB-GENE-950506. ada.
Phylogenomic databases
HOVERGEN	HBG001718.
Family and domain databases
InterPro	IPR006650. A/AMP_deam_AS. IPR001365. A/AMP_deaminase_dom. IPR006330. A_deaminase. [Graphical view]
KO	K01488.
PANTHER	PTHR11409:SF21. PTHR11409:SF21. 1 hit.
Pfam	PF00962. A_deaminase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01430. Aden_deam. 1 hit.
PROSITE	PS00485. A_DEAMINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADA_XENLA

Accession

Primary (citable) accession number: Q6GP70

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	July 19, 2004
Last modified:	December 14, 2011
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program