Q6GNR9 (OST48_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
Short name=DDOST 48 kDa subunit
Short name=Oligosaccharyl transferase 48 kDa subunit
EC=2.4.1.119

Gene names

Name:

ddost

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	438 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains By similarity.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein By similarity.
Sequence similarities	Belongs to the DDOST 48 kDa subunit family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Transferase
Gene Ontology (GO)
Biological process	protein N-linked glycosylation via asparagine Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW oligosaccharyltransferase complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 438	413	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit		PRO_0000357449
Regions
	Topological domain	26 – 408	383	Lumenal Potential
	Transmembrane	409 – 429	21	Helical; Potential
	Topological domain	430 – 438	9	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6GNR9 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 035D5A0AA2E86751	FASTA	438	48,712
	10 20 30 40 50 60 MASLRLSVLL VSVSWLLLLV SGLRAGPRTL VLMENINLRE THSLFFRSLS DRGFDLSFKT 70 80 90 100 110 120 ADDPSLSLIK YGEFLYDNLI IFSPSVEDFG GNINIETISS FIDGGGSVLV AASSDIGDPL 130 140 150 160 170 180 RELGSECGIE FDEEKTAVID HHNYDISDPG QHTLIVADSE NLLKAPTIVG KTPLNPILFR 190 200 210 220 230 240 GVGMVADPDN PLVLDILTGS STSYSFFPDK PITQYPHAVG KNTLLIAGLQ ARNNARVVFS 250 260 270 280 290 300 GSLDFFSDSF FNSAVQKAAS GSNRYAKTGN YELAMALSRW VFKEEGVLRV GEVSHHRVGE 310 320 330 340 350 360 SSPPSAYTVT DLVEYSIVIE KLSDGKWVPF DGDDIQLEFV RIDPFVRTFL KKNGGKYSVQ 370 380 390 400 410 420 FKLPDVYGVF QFKVDYNRLG YTHLYSTTQI SVRPLQHTQY ERFIPSAYPY YASAFSVMFG 430 LFIFSIVFLH MKEKEKSD

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC073434 mRNA. Translation: AAH73434.1.
RefSeq	NP_001085856.1. NM_001092387.1.
UniGene	Xl.35057.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	444283.
KEGG	xla:444283.
Organism-specific databases
CTD	1650.
Xenbase	XB-GENE-5785164. ddost.
Phylogenomic databases
HOVERGEN	HBG053378.
Family and domain databases
InterPro	IPR005013. OligosaccharylTrfase_su_Wbp1. [Graphical view]
KO	K12670.
PANTHER	PTHR10830. DDOST_48kDa. 1 hit.
Pfam	PF03345. DDOST_48kD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OST48_XENLA

Accession

Primary (citable) accession number: Q6GNR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	July 19, 2004
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program