Q6GNL7 (AL1L1_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytosolic 10-formyltetrahydrofolate dehydrogenase
Short name=10-FTHFDH
Short name=FDH
EC=1.5.1.6

Alternative name(s):
Aldehyde dehydrogenase family 1 member L1

Gene names

Name:

aldh1l1

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	902 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + NADP+ + H2O = tetrahydrofolate + CO2 + NADPH.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	In the N-terminal section; belongs to the GART family. In the C-terminal section; belongs to the aldehyde dehydrogenase family. ALDH1L subfamily. Contains 1 acyl carrier domain.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NADP Phosphopantetheine
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	10-formyltetrahydrofolate catabolic process Inferred from electronic annotation. Source: InterPro biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acyl carrier activity Inferred from electronic annotation. Source: InterPro cofactor binding Inferred from electronic annotation. Source: InterPro formyltetrahydrofolate dehydrogenase activity Inferred from electronic annotation. Source: EC hydroxymethyl-, formyl- and related transferase activity Inferred from electronic annotation. Source: InterPro methyltransferase activity Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 902	902	Cytosolic 10-formyltetrahydrofolate dehydrogenase		PRO_0000315999
Regions
	Domain	323 – 392	70	Acyl carrier
	Region	1 – 203	203	GART
	Region	417 – 902	486	Aldehyde dehydrogenase
Sites
	Active site	106	1	Proton donor By similarity
	Active site	673	1	By similarity
	Active site	707	1	By similarity
	Site	142	1	Essential for catalytic activity By similarity
Amino acid modifications
	Modified residue	354	1	O-(pantetheine 4'-phosphoryl)serine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6GNL7 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: C484728096D4C281	FASTA	902	99,982
	10 20 30 40 50 60 MKIAVIGQSL FGREVYRELL KEGHQVVGVF TIPDKNGKAD PLGADAEKDG IPVFKFPRWR 70 80 90 100 110 120 VKGQAIPEVV EKYKALEAEL NVLPFCSQFI PMEVIDCPKH GSIIYHPSIL PRHRGASAIN 130 140 150 160 170 180 WTLMQGDKIG GFTVFWADDG LDTGDILLQR QCEVLPDDTV NTIYNRFLFP EGVKGMVEAV 190 200 210 220 230 240 RLIAEGNAPR IKQPTEGATY DPMQKKENAK INWDQPAEDI HNFIRGNDKV PGAWTVVDDQ 250 260 270 280 290 300 QLTFFGSSFT RNGPAPDGQP LEIPGASRPA LVTKTGLVLF GNDGERLTVK NIQFEDGKMI 310 320 330 340 350 360 PASQYFKTAD SAALQLSEEE QKVSEEIRAA WRRILTNVSE IEDSTDFFKA GAASMDVVRL 370 380 390 400 410 420 VEEVKLKCNG LQLQNEDVYM ATKFEEFIQM VVRRMRGEDG EEELVIDYVE KEINNMTVKI 430 440 450 460 470 480 PHQLFINGQF MDAEGGKSYD TINPTDGTAI CKVSLAQISD IDRAVAAAKE AFENGEWGKM 490 500 510 520 530 540 NPRDRGRLLY RLADLMEEHQ EELATIESID SGAVYTLALK THVGMSIQTF RYFAGWCDKI 550 560 570 580 590 600 QGRTIPINQA RPNRNLTFTR REPIGVCGIV IPWNYPLMML AWKTAACLTA GNTVVLKPAQ 610 620 630 640 650 660 VTPLTALKFA ELSVKAGIPK GVINILPGAG SLIGQRLSDH PDVRKIGFTG STPIGKQIMK 670 680 690 700 710 720 SCAVSNVKKV SLELGGKSPL IIFHDCDLDK AVRMGMSSVF FNKGENCIAA GRLFLEESIH 730 740 750 760 770 780 DEFVKRVVEE VKKMKIGDPL DRSTDHGPQN HKAHLDKLIE YCQTGVKEGG KLVYGGKQVE 790 800 810 820 830 840 RPGFFFEPTI FTDVTDEMFI AKEESFGPVM IISKFNDGDI DGVLKRANDS EFGLASGVFT 850 860 870 880 890 900 KDINKALYVS EKLQAGTVFV NTYNKTDVAA PFGGFKQSGF GKDLGEEALN EYLKTKAVTI EY

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC073490 mRNA. Translation: AAH73490.1.
RefSeq	NP_001085894.1. NM_001092425.1.
UniGene	Xl.5930.
3D structure databases
HSSP	HSSP built from PDB template 1S3I based on UniProtKB P28037.
ProteinModelPortal	Q6GNL7.
SMR	Q6GNL7. Positions 1-401, 405-902.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	444321.
KEGG	xla:444321.
Organism-specific databases
CTD	10840.
Xenbase	XB-GENE-1016244. aldh1l1.
Phylogenomic databases
HOVERGEN	HBG051668.
Family and domain databases
InterPro	IPR011407. 10_FTHF_DH. IPR009081. Acyl_carrier_prot-like. IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR005793. Formyl_trans_C. IPR002376. Formyl_transf_N. IPR011034. Formyl_transferase_C-like. IPR001555. GART_AS. IPR006163. Phsphopanteth-bd. [Graphical view]
Gene3D	G3DSA:1.10.1200.10. ACP_like. 1 hit. G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit. G3DSA:3.10.25.10. Formyl_trans_C. 1 hit. G3DSA:3.40.50.170. Formyl_transf_N. 1 hit.
KO	K00289.
Pfam	PF00171. Aldedh. 1 hit. PF02911. Formyl_trans_C. 1 hit. PF00551. Formyl_trans_N. 1 hit. PF00550. PP-binding. 1 hit. [Graphical view]
PIRSF	PIRSF036489. 10-FTHFDH. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit. SSF50486. FMT_C_like. 1 hit. SSF53328. formyl_transf. 1 hit.
PROSITE	PS50075. ACP_DOMAIN. 1 hit. PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. PS00373. GART. 1 hit. PS00012. PHOSPHOPANTETHEINE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AL1L1_XENLA

Accession

Primary (citable) accession number: Q6GNL7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 19, 2004
Last modified:	November 16, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program