Reviewed, UniProtKB/Swiss-Prot Q6GNI6 (UB22A_XENLA)

Names and origin

Protein names

Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 22 A
    EC=3.1.2.15
Alternative name(s):
    Ubiquitin thioesterase 22 A
    Ubiquitin-specific-processing protease 22 A
    Deubiquitinating enzyme 22 A

Gene names

Name:

usp22-A

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	523 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators, where it is required for transcription By similarity.
Catalytic activity	Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol.
Subunit structure	Component of some SAGA transcription coactivator-HAT complexes By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the peptidase C19 family. UBP8 subfamily. Contains 1 UBP-type zinc finger.

Ontologies

Keywords
Biological process	Cell cycle Transcription Transcription regulation Ubl conjugation pathway
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Activator Chromatin regulator Hydrolase Protease Thiol protease
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 523	523	Ubiquitin carboxyl-terminal hydrolase 22 A		PRO_0000367512
Regions
	Zinc finger	44 – 104	61	UBP-type
Sites
	Active site	183	1	By similarity
	Active site	469	1	By similarity
	Active site	477	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6GNI6-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 731A0AE1C2E67D61	FASTA	523	60,049
	10 20 30 40 50 60 MSPAGCSHVN SFKVENWKQN LRVIYQCFVW SGTPETRKRK AKSCICHMCG AHLNRLHSCL 70 80 90 100 110 120 YCVFFGCFTK KHIHEHAKNK RHNLAIDLLY GGIYCFMCQD YIYDKDMEQV AKEEQRKAWK 130 140 150 160 170 180 LQVFSPALVS PYQYTMTGVG EKYSTWEPTK RELELLQHNP KRRKITTNCT IGLRGLINLG 190 200 210 220 230 240 NTCFMNCIVQ ALTHTPLLRD FFLSDRHKCE MQSPNSCLVC EMSTLFQEFY SGHRSPHIPY 250 260 270 280 290 300 RLLHLVWTHA RHLAGYEQQD AHEFLIAALD VLHRHCKGDD NGKKANNPNH CNCIIDQIFT 310 320 330 340 350 360 GGLQSDVTCQ VCHGVSTTID PFWDISLDLP GSSTPFWPLS PGSDAGVVNG ESHVSGTTTL 370 380 390 400 410 420 TDCLRRFTRP EHLGSSAKIK CSGCHSYQES TKQLTMKKLP IVACFHLKRF EHSAKLRRKI 430 440 450 460 470 480 TTYVSFPLEL DMTPFMASSK ESRMNGQYQQ PSDSLHNDNK YSLFAVVNHQ GTLESGHYTS 490 500 510 520 FIRQHKDQWF KCDDAIITKA SIKDVLDSEG YLLFYHKQFL EYE

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Spleen.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC073524 mRNA. Translation: AAH73524.1.
RefSeq	NP_001085912.1.
UniGene	Xl.25481
3D structure databases
HSSP	HSSP built from PDB template 2AYN based on UniProtKB P54578.
SMR	Q6GNI6. Positions 5-111, 42-119, 172-517.
ModBase	Search...
Protein family/group databases
MEROPS	C19.035.
Genome annotation databases
GeneID	444339.
KEGG	xla:444339.
Organism-specific databases
Xenbase	XB-FEAT-6086059. usp22. XB-FEAT-5806497. usp27x.
Phylogenomic databases
HOVERGEN	Q6GNI6.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR001607. Znf_UBP. [Graphical view]
Pfam	PF00443. UCH. 1 hit. PF02148. zf-UBP. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. PS50271. ZF_UBP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UB22A_XENLA

Accession

Primary (citable) accession number: Q6GNI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 19, 2004
Last modified:	January 19, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project