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Q6GMN2

- BAIP2_RAT

UniProt

Q6GMN2 - BAIP2_RAT

Protein

Brain-specific angiogenesis inhibitor 1-associated protein 2

Gene

Baiap2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (19 Jul 2004)
      Previous versions | rss
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    Functioni

    Adapter protein that links membrane-bound small G-proteins to cytoplasmic effector proteins. Necessary for CDC42-mediated reorganization of the actin cytoskeleton and for RAC1-mediated membrane ruffling. Involved in the regulation of the actin cytoskeleton by WASF family members and the Arp2/3 complex. Plays a role in neurite growth. Acts syngeristically with ENAH to promote filipodia formation. Plays a role in the reorganization of the actin cytoskeleton in response to bacterial infection. Participates in actin bundling when associated with EPS8, promoting filopodial protrusions By similarity.By similarity

    GO - Molecular functioni

    1. proline-rich region binding Source: UniProtKB
    2. protein binding Source: IntAct

    GO - Biological processi

    1. actin crosslink formation Source: UniProtKB
    2. actin filament bundle assembly Source: UniProtKB
    3. dendrite development Source: Ensembl
    4. filopodium assembly Source: InterPro
    5. regulation of actin cytoskeleton organization Source: UniProtKB
    6. regulation of cell shape Source: UniProtKB
    7. regulation of synaptic plasticity Source: Ensembl
    8. response to bacterium Source: UniProtKB
    9. signal transduction Source: InterPro

    Enzyme and pathway databases

    ReactomeiREACT_196755. Regulation of actin dynamics for phagocytic cup formation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Brain-specific angiogenesis inhibitor 1-associated protein 2
    Short name:
    BAI-associated protein 2
    Short name:
    BAI1-associated protein 2
    Alternative name(s):
    Insulin receptor substrate protein of 53 kDa
    Short name:
    IRSp53
    Short name:
    Insulin receptor substrate p53
    Insulin receptor tyrosine kinase substrate protein p53
    Gene namesi
    Name:Baiap2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi619814. Baiap2.

    Subcellular locationi

    Cytoplasm By similarity. Membrane By similarity; Peripheral membrane protein By similarity. Cell projectionfilopodium By similarity. Cell projectionruffle By similarity. Cytoplasmcytoskeleton By similarity
    Note: Detected throughout the cytoplasm in the absence of specific binding partners. Detected in filopodia and close to membrane ruffles. Recruited to actin pedestals that are formed upon infection by bacteria at bacterial attachment sites By similarity.By similarity

    GO - Cellular componenti

    1. actin cytoskeleton Source: Ensembl
    2. cell junction Source: Ensembl
    3. cytosol Source: UniProtKB
    4. filopodium Source: UniProtKB-SubCell
    5. neuron projection Source: Ensembl
    6. nucleus Source: Ensembl
    7. plasma membrane Source: Ensembl
    8. ruffle Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell projection, Cytoplasm, Cytoskeleton, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi17 – 171Y → F: Loss of phosphorylation; when associated with F-115 and F-178. 1 Publication
    Mutagenesisi115 – 1151Y → F: Loss of phosphorylation; when associated with F-17 and F-178. 1 Publication
    Mutagenesisi178 – 1781Y → F: Loss of phosphorylation; when associated with F-17 and F-115. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 535535Brain-specific angiogenesis inhibitor 1-associated protein 2PRO_0000064818Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei324 – 3241PhosphoserineBy similarity
    Modified residuei326 – 3261PhosphoserineBy similarity
    Modified residuei341 – 3411PhosphothreonineBy similarity
    Modified residuei347 – 3471PhosphoserineBy similarity
    Modified residuei367 – 3671PhosphoserineBy similarity
    Modified residuei455 – 4551PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated on tyrosine residues by INSR in response to insulin treatment.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ6GMN2.
    PRIDEiQ6GMN2.

    PTM databases

    PhosphoSiteiQ6GMN2.

    Expressioni

    Tissue specificityi

    Ubiquitous.2 Publications

    Gene expression databases

    GenevestigatoriQ6GMN2.

    Interactioni

    Subunit structurei

    Homodimer. Interacts with CDC42 and RAC1 that have been activated by GTP binding. Binds TIAM1. Interacts with ATN1, BAI1, DIAPH1, EPS8, SHANK1, SHANK2, SHANK3, WASF1 and WASF2. Interacts with ENAH after recruitment of CDC42 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Lin7bQ9Z2523EBI-6997402,EBI-7001699

    Protein-protein interaction databases

    BioGridi250757. 6 interactions.
    IntActiQ6GMN2. 8 interactions.
    MINTiMINT-93063.
    STRINGi10116.ENSRNOP00000005687.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6GMN2.
    SMRiQ6GMN2. Positions 1-248, 379-441.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 250250IMDPROSITE-ProRule annotationAdd
    BLAST
    Domaini375 – 43864SH3PROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili1 – 251251By similarityAdd
    BLAST

    Domaini

    The IMD domain forms a coiled coil. The isolated domain can induce actin bundling and filopodia formation. In the absence of G-proteins intramolecular interaction between the IMD and the SH3 domain gives rise to an auto-inhibited state of the protein. Interaction of the IMD with RAC1 or CDC42 leads to activation By similarity.By similarity
    The SH3 domain interacts with ATN1, BAI1, WASF1, WASF2, SHANK1, DIAPH1 and ENAH.By similarity

    Sequence similaritiesi

    Contains 1 IMD (IRSp53/MIM homology) domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, SH3 domain

    Phylogenomic databases

    eggNOGiNOG71665.
    HOGENOMiHOG000038005.
    HOVERGENiHBG054462.
    KOiK05627.
    PhylomeDBiQ6GMN2.

    Family and domain databases

    InterProiIPR027681. BAIAP2.
    IPR013606. IRSp53/MIM_homology_IMD.
    IPR001452. SH3_domain.
    [Graphical view]
    PANTHERiPTHR14206. PTHR14206. 1 hit.
    PfamiPF08397. IMD. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view]
    SMARTiSM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    PROSITEiPS51338. IMD. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q6GMN2-1) [UniParc]FASTAAdd to Basket

    Also known as: IRS-58

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSLSRSEEMH RLTENVYKTI MEQFNPSLRN FIAMGKNYEK ALAGVTFAAK    50
    GYFDALVKMG ELASESQGSK ELGDVLFQMA EVHRQIQNQL EEMLKAFHNE 100
    LLTQLEQKVE LDSRYLSAAL KKYQTEQRSK GDALDKCQAE LKKLRKKSQG 150
    SKNPQKYSDK ELQYIDAISN KQGELENYVS DGYKTALTEE RRRFCFLVEK 200
    QCAVAKNSAA YHSKGKELLA QKLPLWQQAC ADPNKIPDRA AQLMQQMANS 250
    NGSILPSALS ASKSNLVISD PIPGAKPLPV PPELAPFVGR MSAQENVPVM 300
    NGVAGADSED YNPWADRKAA QPKSLSPPQS QSKLSDSYSN TLPVRKSVTP 350
    KNSYATTENK TLPRSSSMAA GLERNGRMRV KAIFSHAAGD NSTLLSFKEG 400
    DLITLLVPEA RDGWHYGESE KTKMRGWFPF SYTRVLDSDG SDRLHMSLQQ 450
    GKSSSTGNLL DKDDLALPPP DYGTSSRAFP SQTAGTFKQR PYSVAVPAFS 500
    QGLDDYGARS VSRNPFANVH LKPTVTNDRS APLLS 535
    Length:535
    Mass (Da):59,183
    Last modified:July 19, 2004 - v1
    Checksum:i606CED16E5DFD212
    GO
    Isoform 2 (identifier: Q6GMN2-2) [UniParc]FASTAAdd to Basket

    Also known as: IRSp53S, BAIAP2-alpha

    The sequence of this isoform differs from the canonical sequence as follows:
         513-535: RNPFANVHLKPTVTNDRSAPLLS → SGSGTLVSTV

    Show »
    Length:522
    Mass (Da):57,542
    Checksum:iCCFF96819E52B999
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei513 – 53523RNPFA…APLLS → SGSGTLVSTV in isoform 2. 1 PublicationVSP_015510Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY037934 mRNA. Translation: AAK72488.1.
    BC074009 mRNA. Translation: AAH74009.1.
    RefSeqiNP_476544.1. NM_057196.1. [Q6GMN2-2]
    XP_006247931.1. XM_006247869.1. [Q6GMN2-1]
    UniGeneiRn.95155.

    Genome annotation databases

    GeneIDi117542.
    KEGGirno:117542.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY037934 mRNA. Translation: AAK72488.1 .
    BC074009 mRNA. Translation: AAH74009.1 .
    RefSeqi NP_476544.1. NM_057196.1. [Q6GMN2-2 ]
    XP_006247931.1. XM_006247869.1. [Q6GMN2-1 ]
    UniGenei Rn.95155.

    3D structure databases

    ProteinModelPortali Q6GMN2.
    SMRi Q6GMN2. Positions 1-248, 379-441.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 250757. 6 interactions.
    IntActi Q6GMN2. 8 interactions.
    MINTi MINT-93063.
    STRINGi 10116.ENSRNOP00000005687.

    PTM databases

    PhosphoSitei Q6GMN2.

    Proteomic databases

    PaxDbi Q6GMN2.
    PRIDEi Q6GMN2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 117542.
    KEGGi rno:117542.

    Organism-specific databases

    CTDi 10458.
    RGDi 619814. Baiap2.

    Phylogenomic databases

    eggNOGi NOG71665.
    HOGENOMi HOG000038005.
    HOVERGENi HBG054462.
    KOi K05627.
    PhylomeDBi Q6GMN2.

    Enzyme and pathway databases

    Reactomei REACT_196755. Regulation of actin dynamics for phagocytic cup formation.

    Miscellaneous databases

    NextBioi 620361.

    Gene expression databases

    Genevestigatori Q6GMN2.

    Family and domain databases

    InterProi IPR027681. BAIAP2.
    IPR013606. IRSp53/MIM_homology_IMD.
    IPR001452. SH3_domain.
    [Graphical view ]
    PANTHERi PTHR14206. PTHR14206. 1 hit.
    Pfami PF08397. IMD. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view ]
    SMARTi SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    PROSITEi PS51338. IMD. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Insulin receptor substrate protein p53 localization in rats suggests mechanism for specific polyglutamine neurodegeneration."
      Thomas E.A., Foye P.E., Alvarez C.E., Usui H., Sutcliffe J.G.
      Neurosci. Lett. 309:145-148(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
      Strain: Sprague-Dawley.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Lung.
    3. Lubec G., Chen W.-Q.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 12-18 AND 41-50, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Hippocampus.
    4. "Distinctive tissue distribution and phosphorylation of IRSp53 isoforms."
      Okamura-Oho Y., Miyashita T., Yamada M.
      Biochem. Biophys. Res. Commun. 289:957-960(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION, MUTAGENESIS OF TYR-17; TYR-115 AND TYR-178, ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
    5. "Tiam1-IRSp53 complex formation directs specificity of rac-mediated actin cytoskeleton regulation."
      Connolly B.A., Rice J., Feig L.A., Buchsbaum R.J.
      Mol. Cell. Biol. 25:4602-4614(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TIAM1.

    Entry informationi

    Entry nameiBAIP2_RAT
    AccessioniPrimary (citable) accession number: Q6GMN2
    Secondary accession number(s): Q923H3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 30, 2005
    Last sequence update: July 19, 2004
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3