ID   CMAH_DANRE              Reviewed;         591 AA.
AC   Q6GML1;
DT   10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   16-JUN-2009, entry version 38.
DE   RecName: Full=Cytidine monophosphate-N-acetylneuraminic acid hydroxylase;
DE            Short=CMP-N-acetylneuraminic acid hydroxylase;
DE            EC=1.14.18.2;
DE   AltName: Full=CMP-Neu5Ac hydroxylase;
DE   AltName: Full=CMP-NeuAc hydroxylase;
DE   AltName: Full=CMP-N-acetylneuraminate monooxygenase;
GN   Name=cmah; ORFNames=zgc:92407;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Cyprinidae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the conversion of CMP-N-acetylneuraminic acid
CC       (CMP-Neu5Ac) into its hydroxylated derivative CMP-N-
CC       glycolylneuraminic acid (CMP-Neu5Gc), a sialic acid abundantly
CC       expressed at the surface of many cells (By similarity).
CC   -!- CATALYTIC ACTIVITY: CMP-N-acetylneuraminate + 2 ferrocytochrome b5
CC       + O(2) + 2 H(+) = CMP-N-glycoloylneuraminate + 2 ferricytochrome
CC       b5 + H(2)O.
CC   -!- COFACTOR: Binds 1 2Fe-2S cluster per subunit (By similarity).
CC   -!- PATHWAY: Amino-sugar metabolism; N-acetylneuraminic acid
CC       metabolism.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the CMP-Neu5Ac hydroxylase family.
CC   -!- SIMILARITY: Contains 1 Rieske domain.
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DR   EMBL; BC074033; AAH74033.1; -; mRNA.
DR   IPI; IPI00511076; -.
DR   RefSeq; NP_001002192.1; -.
DR   UniGene; Dr.82548; -.
DR   PRIDE; Q6GML1; -.
DR   Ensembl; ENSDARG00000057714; Danio rerio.
DR   Ensembl; ENSDARG00000074972; Danio rerio.
DR   GeneID; 431739; -.
DR   KEGG; dre:431739; -.
DR   ZFIN; ZDB-GENE-040704-33; zgc:92407.
DR   HOVERGEN; Q6GML1; -.
DR   BRENDA; 1.14.18.2; 96826.
DR   Bgee; Q6GML1; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0030338; F:CMP-N-acetylneuraminate monooxygenase activity; IEA:EC.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
DR   GO; GO:0006810; P:transport; IEA:UniProtKB-KW.
DR   InterPro; IPR017941; Rieske_2Fe-2S.
DR   Pfam; PF00355; Rieske; 1.
DR   PROSITE; PS51296; RIESKE; 1.
PE   2: Evidence at transcript level;
KW   2Fe-2S; Cytoplasm; Electron transport; Iron; Iron-sulfur;
KW   Metal-binding; Oxidoreductase; Transport.
FT   CHAIN         1    591       Cytidine monophosphate-N-acetylneuraminic
FT                                acid hydroxylase.
FT                                /FTId=PRO_0000127808.
FT   DOMAIN       12    110       Rieske.
FT   METAL        52     52       Iron-sulfur (2Fe-2S) (By similarity).
FT   METAL        54     54       Iron-sulfur (2Fe-2S); via pros nitrogen
FT                                (By similarity).
FT   METAL        73     73       Iron-sulfur (2Fe-2S) (By similarity).
FT   METAL        76     76       Iron-sulfur (2Fe-2S); via pros nitrogen
FT                                (By similarity).
SQ   SEQUENCE   591 AA;  68297 MW;  9462314CEF46A49E CRC64;
     MAAQVSHTVL RLEAEDVRNL KDGINFQKNN KDGKCYIIYK ANGELRACRN QCKHQGGLFI
     KDIEDMDGRT VRCTKHYWKL NVATMQYVNP PDSFMQDELE AVLSETDGSL ELLELNPPDP
     WTAEPREAQD LQAGEITLTY ITHACMELKA GERRMMFDPW LTGPAFARGW WLLHEPPKDA
     MDRLMEADLV YISHMHSDHL SYPTLQHLSK KRPDIPIYVG NTSRPVFWYL EKSGVNLTNI
     NVVPFGVWQN VDDHLRFMIL MDGVHPEMDT CLIVEYKGHM ILNTVDCTRP NNGRLPHGVD
     VMMSDFAGGA SGFPMTFHGG KYTESWKANF IKNERKKLLN YKAQLVKSLQ PKIYCPFAGY
     FTEAHPSDRY IKETNTKNSP AELNELIRKS CLNTLTWTPL PGSVLDLAVA LNNRSNETAI
     TDPPHGTKIY KDNWEFDLYL NQLNASISAE IFKHKHWIQY YYNWAGFRNY NLVIRVIETD
     DDFQPLNGGF DYLVDFLDLS FPTERPEREH AYEEIKNRVN VMRHVVVNGL LWDDLYIGFN
     NRMSRDPDVY HHKFWNHFQT ELPLSAPDWQ HFLQICSQTQ ENGSSNGCSV S
//