Reviewed, UniProtKB/Swiss-Prot Q6GMK0 (MGT4B_DANRE)

Names and origin

Protein names

Recommended name:
    Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B
    EC=2.4.1.145
Alternative name(s):
    UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVb
    N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVb
      Short name=N-acetylglucosaminyltransferase IVb
      Short name=GlcNAc-T IVb
      Short name=GnT-IVb

Gene names

Name:	mgat4b
ORF Names:	zgc:92425

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	547 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2,4-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.
Cofactor	Divalent metal cations By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 54 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Coiled coil Signal-anchor Transmembrane
Ligand	Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 547	547	Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B		PRO_0000288595
Regions
	Topological domain	1 – 7	7	Cytoplasmic Potential
	Transmembrane	8 – 28	21	Signal-anchor for type II membrane protein Potential
	Topological domain	29 – 547	519	Lumenal Potential
	Coiled coil	36 – 83	48	Potential
Amino acid modifications
	Glycosylation	85	1	N-linked (GlcNAc...) Potential
	Glycosylation	101	1	N-linked (GlcNAc...) Potential
	Glycosylation	464	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6GMK0-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 4C42FDCFA3AD128C	FASTA	547	62,991
	10 20 30 40 50 60 MRLRNGTFLT VLLFGLCGLI SLSWYTAFSN SKGNVVDIYQ REFLALRDRL HSAEQENLKR 70 80 90 100 110 120 SKELNLVLDE IKRAIAEKQA LRDINRTWSS LSDETKLKLW NVTSSKNVLQ LPSIFHHLPH 130 140 150 160 170 180 LLAKESSLQP AVHIGQGRTG VSIVLGVPSV KREVHSYLTD TLSSLMTELS PAEKEDCVIV 190 200 210 220 230 240 VFIAETDQQY ANSVADNLKR LFPGEIQSGL LEIISPSVHF YPDFSHLKES FGDPKERVRW 250 260 270 280 290 300 RTKQNLDYCF LMMYAQTKGN YYVQLEDDIV ARPNYFTTMK NFALQQPSEE WMILEFSQLG 310 320 330 340 350 360 FIGKMFKSLD LPLIVEFMLM FYKDKPIDWL LDHIMWVKVC NPEKDAKHCD RQKANLRIRF 370 380 390 400 410 420 KPSLFQHVGT HSSLAGKIQK LKDKDFGKQT LHKGHANPLA EVTTSLKTYQ HFTLEKAYLG 430 440 450 460 470 480 EDFFWAFTPV AGDFIRIRFF TPVRVERYFF RSGNIEHPGD KLFNTTVEVL PFDNIQSEKE 490 500 510 520 530 540 ALKEGREKTP KYQRTEDGFI RIGKFENGIA EGEVDASFGP LEALRLSVLT DSPVWVILSE IFIKKAE

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC074045 mRNA. Translation: AAH74045.1.
IPI	IPI00496240.
RefSeq	NP_001002180.1.
UniGene	Dr.35822
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6GMK0.
Protein family/group databases
CAZy	GT54. Glycosyltransferase Family 54.
Genome annotation databases
Ensembl	ENSDART00000001159; ENSDARP00000000748; ENSDARG00000004115; Danio rerio. [Genome view]
GeneID	431727.
KEGG	dre:431727.
Organism-specific databases
CTD	431727.
ZFIN	ZDB-GENE-040704-19. mgat4b.
Phylogenomic databases
HOGENOM	Q6GMK0.
HOVERGEN	Q6GMK0.
Enzyme and pathway databases
BRENDA	2.4.1.145. 96826.
Gene expression databases
Bgee	Q6GMK0.
Family and domain databases
InterPro	IPR006759. Glyco_transf_54. [Graphical view]
PANTHER	PTHR12062. Glyco_transf_54. 1 hit.
Pfam	PF04666. Glyco_transf_54. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MGT4B_DANRE

Accession

Primary (citable) accession number: Q6GMK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	July 19, 2004
Last modified:	October 13, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project