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Protein

UDP-glucuronic acid decarboxylase 1

Gene

uxs1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the NAD-dependent decarboxylation of UDP-glucuronic acid to UDP-xylose. Necessary for the biosynthesis of the core tetrasaccharide in glycosaminoglycan biosynthesis (By similarity). Essential during embryogenesis for craniofacial development.By similarity2 Publications

Catalytic activityi

UDP-D-glucuronate = UDP-D-xylose + CO2.

Cofactori

NAD+By similarity

Pathwayi: UDP-alpha-D-xylose biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes UDP-alpha-D-xylose from UDP-alpha-D-glucuronate.
Proteins known to be involved in this subpathway in this organism are:
  1. UDP-glucuronic acid decarboxylase 1 (uxs1)
This subpathway is part of the pathway UDP-alpha-D-xylose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP-alpha-D-xylose from UDP-alpha-D-glucuronate, the pathway UDP-alpha-D-xylose biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei226SubstrateBy similarity1
Active sitei229Proton acceptorBy similarity1
Binding sitei258SubstrateBy similarity1
Binding sitei270NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi117 – 142NADBy similarityAdd BLAST26
Nucleotide bindingi229 – 233NADBy similarity5
Nucleotide bindingi271 – 275SubstrateBy similarity5
Nucleotide bindingi288 – 295SubstrateBy similarity8
Nucleotide bindingi355 – 359SubstrateBy similarity5

GO - Molecular functioni

GO - Biological processi

  • cartilage development Source: ZFIN
  • chondroitin sulfate biosynthetic process Source: ZFIN
  • chondroitin sulfate proteoglycan biosynthetic process Source: ZFIN
  • extracellular matrix organization Source: ZFIN
  • heparan sulfate proteoglycan biosynthetic process Source: ZFIN
  • ossification Source: ZFIN
  • proteoglycan biosynthetic process Source: ZFIN
  • UDP-D-xylose biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Developmental protein, Lyase

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00796; UER00771.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glucuronic acid decarboxylase 1 (EC:4.1.1.35)
Alternative name(s):
UDP-glucuronate decarboxylase 1
Short name:
UXS-1
Gene namesi
Name:uxs1
ORF Names:zgc:91980
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 9

Organism-specific databases

ZFINiZDB-GENE-020419-37. uxs1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 17CytoplasmicSequence analysisAdd BLAST17
Transmembranei18 – 38Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini39 – 418LumenalSequence analysisAdd BLAST380

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutants show cartilage defects due to impaired chondrogenesis and have a reduced jaw size.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001832731 – 418UDP-glucuronic acid decarboxylase 1Add BLAST418

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi314N-linked (GlcNAc...)Sequence analysis1
Glycosylationi383N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ6GMI9.

Expressioni

Gene expression databases

BgeeiENSDARG00000056102.
ExpressionAtlasiQ6GMI9. differential.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000072986.

Structurei

3D structure databases

ProteinModelPortaliQ6GMI9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1429. Eukaryota.
COG0451. LUCA.
GeneTreeiENSGT00530000063128.
HOGENOMiHOG000168004.
HOVERGENiHBG094144.
InParanoidiQ6GMI9.
KOiK08678.
OMAiPKVKYLN.
OrthoDBiEOG091G0BV7.
PhylomeDBiQ6GMI9.
TreeFamiTF105736.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR021761. UXS1_N.
[Graphical view]
PfamiPF16363. GDP_Man_Dehyd. 1 hit.
PF11803. UXS1_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6GMI9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMRMSWMVTV INRRMMKILI ALALIAYIAS VWGTYANMRS IQEHGEMKIE
60 70 80 90 100
QRIDEAVGPL REKIRELELS FTQKYPPVKF LSEKDRKRIL ITGGAGFVGS
110 120 130 140 150
HLTDKLMMDG HEVTVVDNFF TGRKRNVEHW IGHENFELIN HDVVEPLYIE
160 170 180 190 200
VDQIYHLASP ASPPNYMYNP IKTLKTNTIG TLNMLGLAKR VGARLLLAST
210 220 230 240 250
SEVYGDPEVH PQNEDYWGHV NPIGPRACYD EGKRVAETMC YAYMKQEGVE
260 270 280 290 300
VRVARIFNTF GSRMHMNDGR VVSNFILQAL QGEALTVYGS GSQTRAFQYV
310 320 330 340 350
SDLVNGLVSL MNSNISSPVN LGNPEEHTIL EFAQLIKSLV ASRSHIQFLP
360 370 380 390 400
EAQDDPQRRR PDIRKAKLLL GWEPVVPLEE GLNKTIQYFS RELEHQANNQ
410
YIPKPKAARM KKGRPRHN
Length:418
Mass (Da):47,676
Last modified:December 20, 2005 - v2
Checksum:iA07B28880A7D7EC0
GO

Sequence cautioni

The sequence AAH74058 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti333 – 334AQ → GS in AAM34679 (PubMed:12006978).Curated2
Sequence conflicti350P → S in AAM34679 (PubMed:12006978).Curated1
Sequence conflicti361P → T in AAM34679 (PubMed:12006978).Curated1
Sequence conflicti365K → R in AAM34679 (PubMed:12006978).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF506235 mRNA. Translation: AAM34679.1.
BC074058 mRNA. Translation: AAH74058.1. Different initiation.
RefSeqiNP_775349.2. NM_173242.2.
UniGeneiDr.79684.

Genome annotation databases

EnsembliENSDART00000078525; ENSDARP00000072986; ENSDARG00000056102.
GeneIDi192315.
KEGGidre:192315.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF506235 mRNA. Translation: AAM34679.1.
BC074058 mRNA. Translation: AAH74058.1. Different initiation.
RefSeqiNP_775349.2. NM_173242.2.
UniGeneiDr.79684.

3D structure databases

ProteinModelPortaliQ6GMI9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000072986.

Proteomic databases

PaxDbiQ6GMI9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000078525; ENSDARP00000072986; ENSDARG00000056102.
GeneIDi192315.
KEGGidre:192315.

Organism-specific databases

CTDi80146.
ZFINiZDB-GENE-020419-37. uxs1.

Phylogenomic databases

eggNOGiKOG1429. Eukaryota.
COG0451. LUCA.
GeneTreeiENSGT00530000063128.
HOGENOMiHOG000168004.
HOVERGENiHBG094144.
InParanoidiQ6GMI9.
KOiK08678.
OMAiPKVKYLN.
OrthoDBiEOG091G0BV7.
PhylomeDBiQ6GMI9.
TreeFamiTF105736.

Enzyme and pathway databases

UniPathwayiUPA00796; UER00771.

Miscellaneous databases

PROiQ6GMI9.

Gene expression databases

BgeeiENSDARG00000056102.
ExpressionAtlasiQ6GMI9. differential.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR021761. UXS1_N.
[Graphical view]
PfamiPF16363. GDP_Man_Dehyd. 1 hit.
PF11803. UXS1_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUXS1_DANRE
AccessioniPrimary (citable) accession number: Q6GMI9
Secondary accession number(s): Q8JHG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 30, 2016
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-2 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.