Q6GMI7 (ENOPH_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Enolase-phosphatase E1
EC=3.1.3.77

Alternative name(s):
2,3-diketo-5-methylthio-1-phosphopentane phosphatase
MASA homolog

Gene names

Name:	enoph1
Synonyms:	masa
ORF Names:	zgc:91991

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) By similarity.
Catalytic activity	5-(methylthio)-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6. Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. MasA/mtnC family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Cellular component	Cytoplasm Nucleus
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	L-methionine salvage from methylthioadenosine Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity Inferred from electronic annotation. Source: InterPro 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase activity Inferred from electronic annotation. Source: InterPro acireductone synthase activity Inferred from sequence or structural similarity. Source: UniProtKB magnesium ion binding Inferred from electronic annotation. Source: InterPro phosphoglycolate phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 261	261	Enolase-phosphatase E1		PRO_0000254010
Regions
	Region	153 – 154	2	Substrate binding By similarity
Sites
	Metal binding	16	1	Magnesium By similarity
	Metal binding	18	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	212	1	Magnesium By similarity
	Binding site	187	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6GMI7 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: F4B4F2DADD03DE46	FASTA	261	29,978
	10 20 30 40 50 60 MAAIAIPENT RVFLLDIEGT TTPITFVKDI LFPYIRENLE DYLSAHWEED ECKQDVHLLK 70 80 90 100 110 120 KQTEEDLRQN KACHVHTVDQ TVHTDEEKAI REVVENVLWQ MAADRKTTAL KQLQGHMWRA 130 140 150 160 170 180 AYMMGRIKGE VYQDVVPAIR RWRHHGLKIY IYSSGSVEAQ KLLFGYSVQG DILDLFDGHF 190 200 210 220 230 240 DTNIGAKVES KSYENIAERI GCQPEEIMFL TDVTREAKAA EDAGVNVAVV VRPGNMELTE 250 260 EERDHYRIIT SFNQLELIGN V

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC074060 mRNA. Translation: AAH74060.1.
IPI	IPI00490093.
RefSeq	NP_001002226.1. NM_001002226.1.
UniGene	Dr.19647.
3D structure databases
HSSP	HSSP built from PDB template 1ZS9 based on UniProtKB Q9UHY7.
ProteinModelPortal	Q6GMI7.
SMR	Q6GMI7. Positions 4-257.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6GMI7.
Proteomic databases
PRIDE	Q6GMI7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000031165; ENSDARP00000031494; ENSDARG00000026198.
GeneID	431773.
KEGG	dre:431773.
Organism-specific databases
CTD	58478.
ZFIN	ZDB-GENE-040704-73. enoph1.
Phylogenomic databases
eggNOG	fiNOG04971.
GeneTree	ENSGT00440000039914.
HOGENOM	HBG294242.
HOVERGEN	HBG054539.
InParanoid	Q6GMI7.
OMA	TTDLNFI.
OrthoDB	EOG405S1X.
PhylomeDB	Q6GMI7.
Gene expression databases
ArrayExpress	Q6GMI7.
Bgee	Q6GMI7.
Family and domain databases
InterPro	IPR005834. Dehalogen-like_hydro. IPR023943. Enolase-phosphatase_E1. IPR010041. Enolase_ppase. IPR023214. HAD-like_dom. IPR006439. HAD-SF_hydro_IA_v1. [Graphical view]
Gene3D	G3DSA:3.40.50.1000. HAD-like_dom. 1 hit.
KO	K09880.
Pfam	PF00702. Hydrolase. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01691. Enolase-ppase. 1 hit. TIGR01549. HAD-SF-IA-v1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ENOPH_DANRE

Accession

Primary (citable) accession number: Q6GMI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	July 19, 2004
Last modified:	November 16, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program