Q6GMH3 (TWF2_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Twinfilin-2

Alternative name(s):
Twinfilin-1-like protein

Gene names

Name:	twf2
Synonyms:	ptk9l, twf1l
ORF Names:	zgc:91817

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Danio

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin By similarity.
Subunit structure	Interacts with G-actin; ADP-actin form and capping protein (CP) By similarity.
Subcellular location	Cytoplasm › cytoskeleton By similarity. Cytoplasm › perinuclear region By similarity. Note: Perinuclear and G-actin-rich cortical actin structure sublocalization By similarity.
Sequence similarities	Belongs to the actin-binding proteins ADF family. Twinfilin subfamily. Contains 2 ADF-H domains.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Domain	Repeat
Ligand	Actin-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Twinfilin-2		PRO_0000233140
Regions
	Domain	3 – 137	135	ADF-H 1
	Domain	175 – 311	137	ADF-H 2

Sequences

	Sequence		Length	Mass (Da)
	Q6GMH3 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 948D3BCD3A0D7B21	FASTA	347	39,859
	10 20 30 40 50 60 MFLVLVVTEE LREFLARARN GTGRLIQVLI RDEQLVLGAY REPRHSWDKD YDPVLLPLLD 70 80 90 100 110 120 PLEPCYILYR LDSKNAQGYE WLFISWSPDQ SPVRQKMLYA ATRATVKKEF GGGHVKDEMF 130 140 150 160 170 180 GTVEEDICLQ GYLRHITSCS APAPLTVAEQ ELQRIKITEV KAEISVDPKH QTLQGLAFPL 190 200 210 220 230 240 QAEAKRALKQ LAERRINYIQ LKLDTEKETI DLVHTSPTDI RDLPCRIPLD TPRYHFFLYK 250 260 270 280 290 300 HSHEGDYLES VVFIYSMPGY SCSIKERMLY SSCKSRLLDE VERDFHLEVA KKLEIDSGEE 310 320 330 340 LTEEYLYDEV HPKQHAHKQA FAKPRGPAGK RGNKRLIKGG GENGGNS

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Web resources

Protein Spotlight

Molecular embrace - Issue 73 of August 2006

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC074077 mRNA. Translation: AAH74077.1.
IPI	IPI00482215.
RefSeq	NP_001009897.1. NM_001009897.1.
UniGene	Dr.155497.
3D structure databases
ProteinModelPortal	Q6GMH3.
SMR	Q6GMH3. Positions 8-135, 159-311.
ModBase	Search...
Proteomic databases
PRIDE	Q6GMH3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000065006; ENSDARP00000065005; ENSDARG00000094792.
GeneID	100310784.
KEGG	dre:100310784.
Organism-specific databases
CTD	11344.
ZFIN	ZDB-GENE-030131-7638. twf2a.
Phylogenomic databases
eggNOG	COG2319.
GeneTree	ENSGT00530000063868.
HOGENOM	HOG000168296.
HOVERGEN	HBG000848.
KO	K08870.
OMA	SHNAQGF.
Gene expression databases
Bgee	Q6GMH3.
Family and domain databases
InterPro	IPR002108. Actin-bd_cofilin/tropomyosin. [Graphical view]
Pfam	PF00241. Cofilin_ADF. 2 hits. [Graphical view]
SMART	SM00102. ADF. 2 hits. [Graphical view]
PROSITE	PS51263. ADF_H. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TWF2_DANRE

Accession

Primary (citable) accession number: Q6GMH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	July 19, 2004
Last modified:	March 6, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program