Reviewed, UniProtKB/Swiss-Prot Q6GM53 (IKKA_XENLA)

Names and origin

Protein names

Recommended name:
    Inhibitor of nuclear factor kappa-B kinase subunit alpha
      Short name=I kappa-B kinase alpha
      Short name=IkBKA
      Short name=IKK-alpha
      Short name=IKK-A
    EC=2.7.11.10
Alternative name(s):
    Conserved helix-loop-helix ubiquitous kinase
    Nuclear factor NF-kappa-B inhibitor kinase alpha
      Short name=NFKBIKA

Gene names

Name:	chuk
Synonyms:	ikka

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	743 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines By similarity.
Catalytic activity	ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].
Enzyme regulation	Activated when phosphorylated and inactivated when dephosphorylated By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Note: Shuttles between the cytoplasm and the nucleus By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW IkappaB kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 743	743	Inhibitor of nuclear factor kappa-B kinase subunit alpha		PRO_0000268828
Regions
	Domain	15 – 300	286	Protein kinase
	Domain	453 – 474	22	Leucine-zipper Potential
	Nucleotide binding	21 – 29	9	ATP By similarity
	Region	736 – 741	6	NEMO-binding By similarity
Sites
	Active site	144	1	Proton acceptor By similarity
	Binding site	44	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6GM53-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 7FB7B4FA325B1860	FASTA	743	85,152
	10 20 30 40 50 60 MERPPVQHGA VCGPWDMKDR LGTGGFGNVC LYQNRETGEL KAIKSCRLEL SVKNKERWCQ 70 80 90 100 110 120 EIQIMKRLNH PNVVKACDVP PEMDFLVNDV PHLAMEYCAG GDLRKLLNKP ENCCGLKESQ 130 140 150 160 170 180 VLNLISDIGS GIQYLHENRI IHRDLKPENI VLQECNGKTV HKIIDLGYAK DLDQGSLCTS 190 200 210 220 230 240 FVGTLQYLAP ELFENKPYSV TVDYWSFGTM AFECIAGFRP FLHNMQPFTW HEKIKKKDHK 250 260 270 280 290 300 HIYAYEEMSG EIRFSTSLPE PNNLCSIIVD KIETWLQLLL NWDPVQRGGG IDCGRPRCFM 310 320 330 340 350 360 LMDQIFSLKI VHILNMTSAK IHSFHLQPEE SLHTLQSRIE RETGISTCNQ ELLLEMGVSL 370 380 390 400 410 420 DPRKPASQCV IDGVKGWDSY MVYLFDKSKT VYEGPFQSRS LSECLNYIVQ DSKVQLPIPQ 430 440 450 460 470 480 LRKVWAEAVH YVSGLKEDYS RLFQGQRAAM LSLLRFNTNL TKMKNTMVSA SQQLDAKLQF 490 500 510 520 530 540 FKRSIEIDLE RYSDQMAYGI SSEKMLRAWK EMEDKAVCFG KAGEIHFLDE TIMGLHTEIV 550 560 570 580 590 600 ELQRSPCARR QGDVMEHLEQ RAMELYKQLK PRSPDKAYSD STEMVKILVQ TVQGQDRVLK 610 620 630 640 650 660 ELFGHLSKLL GCKQKIIDLL PQIEMTVNNI KEADSSLMQM QAKRQREIWH LLKIACTQSS 670 680 690 700 710 720 TRSLVTASGE TPITSRTSAW SDQSSPQALF SMLTSKDKGR EILRHTIDKN TDYQKLLSNL 730 740 ILQTQTTMEQ TSLMSMDFSW LNQ

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC074232 mRNA. Translation: AAH74232.1.
RefSeq	NP_001086127.1.
UniGene	Xl.31871
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	444556.
KEGG	xla:444556.
Organism-specific databases
Xenbase	XB-FEAT-5910007. chuk.
Phylogenomic databases
HOVERGEN	Q6GM53.
Enzyme and pathway databases
BRENDA	2.7.11.10. 648.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IKKA_XENLA

Accession

Primary (citable) accession number: Q6GM53

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	July 19, 2004
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project