Q6GL44 (OTUD5_XENTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
OTU domain-containing protein 5
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme A
Short name=DUBA

Gene names

Name:

otud5

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) [Reference proteome]

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	518 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Deubiquitinating enzyme that may function as negative regulator of the innate immune system. Has peptidase activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Can also cleave 'Lys-11'-linked ubiquitin chains (in vitro) By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Belongs to the peptidase C85 family. Contains 1 OTU domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein K48-linked deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein K63-linked deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB proteolysis Inferred from electronic annotation. Source: UniProtKB-KW response to lipopolysaccharide Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ubiquitin-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 518	518	OTU domain-containing protein 5		PRO_0000278229
Regions
	Domain	171 – 294	124	OTU
	Compositional bias	5 – 71	67	Pro-rich
	Compositional bias	92 – 102	11	Cys-rich
Sites
	Active site	179	1	Potential
	Active site	182	1	Nucleophile By similarity
	Active site	287	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6GL44 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 69368C93932208F4	FASTA	518	56,332
	10 20 30 40 50 60 MTILPKKKPP PPSDPEGNGE RSGSGGADSH SRSGARPRSS PPPRWAYPGN PAPPERHPAG 70 80 90 100 110 120 TPRPQQASPP PCSGGSGGAG SGPLGEGALG PCCCCTGAGG CCSGPGHSKR RRQGVSAGPG 130 140 150 160 170 180 APGSSPDRED GAGNNSEDEY ETAARTQAID PDTAEQQEHW FEKALQEKKG FIIKQMKEDG 190 200 210 220 230 240 ACLFRAVADQ VYGDQDMHEV VRKHCMDYLM KNADYFSNYV TEDFTTYINR KRKNNCHGNH 250 260 270 280 290 300 IEMQAMAEMY NRPVEVYQYG TEPINTFHGI QQNEDEPIRV SYHRNIHYNS VVNPNKATIG 310 320 330 340 350 360 VGLGLPSFKP GFAEQSLMKS AIRTSEESWI EQQMLEDKKR ATDWEATNEA IEEQVARESY 370 380 390 400 410 420 LQWLRDQEKQ ARQPRKASAT CSSATAAASS GLEEWSGRSP RQRSTAGSPE HPDLHAELCM 430 440 450 460 470 480 KPPSPAATLM LGKPPSPCAP GPSNQTCAGA DRATSPLVSL YPALECRAIM QHMSPTAFGL 490 500 510 KDWDDDEILA SVLAVSQQEY LDTIKKSTLR RDSSPDHS

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC074667 mRNA. Translation: AAH74667.1.
RefSeq	NP_001004849.1. NM_001004849.1.
UniGene	Str.8037.
3D structure databases
ProteinModelPortal	Q6GL44.
ModBase	Search...
Protein family/group databases
MEROPS	C85.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	448134.
KEGG	xtr:448134.
Organism-specific databases
CTD	55593.
Xenbase	XB-GENE-6455884. otud5.
Phylogenomic databases
HOVERGEN	HBG060214.
KO	K12655.
Family and domain databases
InterPro	IPR003323. OTU. [Graphical view]
Pfam	PF02338. OTU. 1 hit. [Graphical view]
PROSITE	PS50802. OTU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTUD5_XENTR

Accession

Primary (citable) accession number: Q6GL44

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	July 19, 2004
Last modified:	July 11, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program