Q6GL33 (PGAM5_XENTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase PGAM5, mitochondrial
EC=3.1.3.16

Alternative name(s):
Phosphoglycerate mutase family member 5

Gene names

Name:

pgam5

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) [Reference proteome]

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Displays phosphatase activity for serine/threonine residues. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics By similarity. May be a central mediator for programmed necrosis By similarity.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Domain	The N-terminal 35 amino acids, including the potential transmembrane alpha-helix, function as a non-cleaved mitochondrial targeting sequence that targets the protein to the cytosolic side of the outer mitochondrial membrane By similarity.
Post-translational modification	Phosphorylated by the RIPK1/RIPK3 complex under necrotic conditions. This phosphorylation increases PGAM5 phosphatase activity By similarity.
Sequence similarities	Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.

Ontologies

Keywords
Biological process	Necrosis
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	phosphoprotein phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	Serine/threonine-protein phosphatase PGAM5, mitochondrial		PRO_0000288787
Regions
	Transmembrane	7 – 27	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6GL33 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 67923E297F554639	FASTA	278	31,211
	10 20 30 40 50 60 MYLRKALIAG GSAAAAAAAI LGAAAVGKSK GGPDLDILSV VPAATGQWDR NWDRREPISM 70 80 90 100 110 120 VNLSKINGET GEEELQLHLN KHKPKATRHI FLIRHSQYKQ DGKTDFDRVL TPLGREQADL 130 140 150 160 170 180 TGKRLSSLGF KYNHIVYSTM TRAKETTEII SKYLPDVKKS SSDLLREGAP IRPEPQVCHW 190 200 210 220 230 240 KPDFVYYEDG SRIEAAFRHF IHRADPKQEA DSYEILICHA NVIRYIVCRA LQLPPEAWLR 250 260 270 MFLNNGSISY LVIRPNGNVS LRMLGDSGFM PPEKISRT

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC074682 mRNA. Translation: AAH74682.1.
RefSeq	NP_001004858.1. NM_001004858.1.
UniGene	Str.1334.
3D structure databases
ProteinModelPortal	Q6GL33.
ModBase	Search...
Protein-protein interaction databases
STRING	8364.ENSXETP00000051391.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	448148.
KEGG	xtr:448148.
Organism-specific databases
CTD	192111.
Xenbase	XB-GENE-948920. pgam5.
Phylogenomic databases
eggNOG	NOG71348.
HOGENOM	HOG000261217.
HOVERGEN	HBG105576.
InParanoid	Q6GL33.
KO	K15637.
OrthoDB	EOG4T4CW2.
Family and domain databases
InterPro	IPR013078. His_Pase_superF_clade-1. [Graphical view]
Pfam	PF00300. His_Phos_1. 1 hit. [Graphical view]
SMART	SM00855. PGAM. 1 hit. [Graphical view]
PROSITE	PS00175. PG_MUTASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGAM5_XENTR

Accession

Primary (citable) accession number: Q6GL33

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	July 19, 2004
Last modified:	April 3, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program