Q6GJK9 (SLE1_STAAR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetylmuramoyl-L-alanine amidase sle1
EC=3.5.1.28

Gene names

Name:	sle1
Synonyms:	aaa
Ordered Locus Names:	SAR0464

Organism

Staphylococcus aureus (strain MRSA252) [Complete proteome] [HAMAP]

Taxonomic identifier

282458 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Peptidoglycan hydrolase involved in the splitting of the septum during cell division By similarity.
Catalytic activity	Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
Subcellular location	Secreted By similarity. Cell surface By similarity.
Sequence similarities	Contains 3 LysM repeats. Contains 1 peptidase C51 domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell wall biogenesis/degradation Septation Virulence
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Antimicrobial Bacteriolytic enzyme Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	barrier septum assembly Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell surface Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	N-acetylmuramoyl-L-alanine amidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 334	309	N-acetylmuramoyl-L-alanine amidase sle1		PRO_0000231624
Regions
	Repeat	29 – 71	43	LysM 1
	Repeat	93 – 135	43	LysM 2
	Repeat	160 – 202	43	LysM 3
	Domain	210 – 334	125	Peptidase C51

Sequences

	Sequence		Length	Mass (Da)
	Q6GJK9 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: A168D7E94420E85A	FASTA	334	36,024
	10 20 30 40 50 60 MQKKVIAAII GTSAISAVAA TQANAATTHT VKPGESVWAI SNKYGISIAK LKSLNNLTSN 70 80 90 100 110 120 LIFPNQVLKV SGSSNSTSNS SRPSTNSGGG SYYTVQAGDS LSLIASKYGT TYQNIMRLNG 130 140 150 160 170 180 LNNFFIYPGQ KLKVSGTASS SNSTSNSSRP STNSSGGSYY TVQAGDSLSL IASKYGTTYQ 190 200 210 220 230 240 NIMRLNGLNN FFIYPGQKLK VTGNASTNSG STTTTNRGYN TPVFNHQNLY TWGQCTYHVF 250 260 270 280 290 300 NRRAEIGKGI STYWWNANNW DNAAAADGYT IDNRPTVGSI AQTDVGYYGH VMFVERVNND 310 320 330 GSILVSEMNY SAAPGILTYR TVPAYQVNNY RYIH

References

[1]

"Complete genomes of two clinical Staphylococcus aureus strains: evidence for the rapid evolution of virulence and drug resistance." Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J., Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A., Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C., Clark L., Corton C. , Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z., Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N., Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M., Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MRSA252.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571856 Genomic DNA. Translation: CAG39485.1.
RefSeq	YP_039913.1. NC_002952.2.
3D structure databases
ProteinModelPortal	Q6GJK9.
ModBase	Search...
Protein family/group databases
CAZy	CBM50. Carbohydrate-Binding Module Family 50.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAG39485; CAG39485; SAR0464.
GeneID	2859570.
KEGG	sar:SAR0464.
PATRIC	19544440. VBIStaAur71814_0470.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3942.
HOGENOM	HOG000279823.
OMA	LIASKYN.
ProtClustDB	CLSK884669.
Enzyme and pathway databases
BioCyc	SAUR282458:GJA5-463-MONOMER.
Family and domain databases
InterPro	IPR007921. CHAP. IPR018392. Peptidoglycan-bd_lysin. IPR002482. Peptidoglycan-bd_Lysin_subgr. [Graphical view]
Pfam	PF05257. CHAP. 1 hit. PF01476. LysM. 3 hits. [Graphical view]
SMART	SM00257. LysM. 3 hits. [Graphical view]
PROSITE	PS50911. CHAP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SLE1_STAAR

Accession

Primary (citable) accession number: Q6GJK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	July 19, 2004
Last modified:	May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program