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Protein

Glyceraldehyde-3-phosphate dehydrogenase 1

Gene

gapA1

Organism
Staphylococcus aureus (strain MRSA252)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

Pathway: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 1 (gapA1), Glyceraldehyde-3-phosphate dehydrogenase 2 (gapA2)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA)
  4. Enolase (eno)
  5. Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei34 – 341NADBy similarity
Active sitei151 – 1511NucleophilePROSITE-ProRule annotation
Sitei178 – 1781Activates thiol group during catalysisBy similarity
Binding sitei181 – 1811Glyceraldehyde 3-phosphateBy similarity
Binding sitei198 – 1981Glyceraldehyde 3-phosphateBy similarity
Binding sitei234 – 2341Glyceraldehyde 3-phosphateBy similarity
Binding sitei316 – 3161NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 132NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciSAUR282458:GJA5-831-MONOMER.
BRENDAi1.2.1.12. 3352.
UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase 1 (EC:1.2.1.12)
Short name:
GAPDH 1
Gene namesi
Name:gapA1
Synonyms:gap, gapA
Ordered Locus Names:SAR0828
OrganismiStaphylococcus aureus (strain MRSA252)
Taxonomic identifieri282458 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 336336Glyceraldehyde-3-phosphate dehydrogenase 1PRO_0000145685Add
BLAST

Proteomic databases

PRIDEiQ6GIL8.

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

Secondary structure

1
336
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 86Combined sources
Helixi12 – 2211Combined sources
Beta strandi27 – 337Combined sources
Helixi38 – 469Combined sources
Turni49 – 513Combined sources
Beta strandi58 – 614Combined sources
Beta strandi64 – 674Combined sources
Beta strandi70 – 756Combined sources
Helixi80 – 823Combined sources
Turni85 – 895Combined sources
Beta strandi91 – 955Combined sources
Beta strandi97 – 993Combined sources
Helixi103 – 1119Combined sources
Beta strandi115 – 1217Combined sources
Beta strandi124 – 1263Combined sources
Turni132 – 1343Combined sources
Helixi136 – 1383Combined sources
Beta strandi144 – 1474Combined sources
Helixi151 – 16717Combined sources
Beta strandi169 – 17911Combined sources
Beta strandi186 – 1883Combined sources
Turni196 – 1994Combined sources
Helixi202 – 2043Combined sources
Beta strandi207 – 2104Combined sources
Turni213 – 2164Combined sources
Helixi217 – 2193Combined sources
Helixi222 – 2243Combined sources
Beta strandi227 – 23610Combined sources
Beta strandi241 – 25313Combined sources
Helixi256 – 26510Combined sources
Beta strandi269 – 2746Combined sources
Helixi280 – 2834Combined sources
Beta strandi289 – 2935Combined sources
Helixi294 – 2963Combined sources
Beta strandi298 – 3025Combined sources
Beta strandi305 – 31410Combined sources
Helixi318 – 33417Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3HQ4X-ray2.20O/P/Q/R1-336[»]
3K73X-ray2.50O/P/Q/R1-336[»]
3K9QX-ray2.50O/P/Q/R1-336[»]
3KSDX-ray2.20O/P/Q/R1-336[»]
3KSZX-ray2.60O/P/Q/R1-336[»]
3KV3X-ray2.50O/P/Q/R1-336[»]
3L4SX-ray2.20O/P/Q/R1-336[»]
3L6OX-ray2.20O/P/Q/R1-336[»]
3LC1X-ray2.00O/P/Q/R1-336[»]
3LC2X-ray2.80O/P/Q/R1-336[»]
3LC7X-ray2.50O/P/Q/R1-336[»]
3LVFX-ray1.70O/P/Q/R1-336[»]
3VAZX-ray3.19A/B/O/P/Q/R1-336[»]
ProteinModelPortaliQ6GIL8.
SMRiQ6GIL8. Positions 1-336.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6GIL8.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni150 – 1523Glyceraldehyde 3-phosphate bindingBy similarity
Regioni211 – 2122Glyceraldehyde 3-phosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0057.
HOGENOMiHOG000071678.
KOiK00134.
OMAiFVRVLSW.
OrthoDBiEOG66TG3S.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6GIL8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVKVAINGF GRIGRLAFRR IQEVEGLEVV AVNDLTDDDM LAHLLKYDTM
60 70 80 90 100
QGRFTGEVEV VDGGFRVNGK EVKSFSEPDA SKLPWKDLNI DVVLECTGFY
110 120 130 140 150
TDKDKAQAHI EAGAKKVLIS APATGDLKTI VFNTNHQELD GSETVVSGAS
160 170 180 190 200
CTTNSLAPVA KVLNDDFGLV EGLMTTIHAY TGDQNTQDAP HRKGDKRRAR
210 220 230 240 250
AAAENIIPNS TGAAKAIGKV IPEIDGKLDG GAQRVPVATG SLTELTVVLE
260 270 280 290 300
KQDVTVEQVN EAMKNASNES FGYTEDEIVS SDVVGMTYGS LFDATQTRVM
310 320 330
SVGDRQLVKV AAWYDNEMSY TAQLVRTLAY LAELSK
Length:336
Mass (Da):36,281
Last modified:July 19, 2004 - v1
Checksum:i37A6CEA9376779E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571856 Genomic DNA. Translation: CAG39837.1.
RefSeqiWP_000279414.1. NC_002952.2.
YP_040254.1. NC_002952.2.

Genome annotation databases

EnsemblBacteriaiCAG39837; CAG39837; SAR0828.
GeneIDi23196651.
KEGGisar:SAR0828.
PATRICi19545193. VBIStaAur71814_0829.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571856 Genomic DNA. Translation: CAG39837.1.
RefSeqiWP_000279414.1. NC_002952.2.
YP_040254.1. NC_002952.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3HQ4X-ray2.20O/P/Q/R1-336[»]
3K73X-ray2.50O/P/Q/R1-336[»]
3K9QX-ray2.50O/P/Q/R1-336[»]
3KSDX-ray2.20O/P/Q/R1-336[»]
3KSZX-ray2.60O/P/Q/R1-336[»]
3KV3X-ray2.50O/P/Q/R1-336[»]
3L4SX-ray2.20O/P/Q/R1-336[»]
3L6OX-ray2.20O/P/Q/R1-336[»]
3LC1X-ray2.00O/P/Q/R1-336[»]
3LC2X-ray2.80O/P/Q/R1-336[»]
3LC7X-ray2.50O/P/Q/R1-336[»]
3LVFX-ray1.70O/P/Q/R1-336[»]
3VAZX-ray3.19A/B/O/P/Q/R1-336[»]
ProteinModelPortaliQ6GIL8.
SMRiQ6GIL8. Positions 1-336.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ6GIL8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAG39837; CAG39837; SAR0828.
GeneIDi23196651.
KEGGisar:SAR0828.
PATRICi19545193. VBIStaAur71814_0829.

Phylogenomic databases

eggNOGiCOG0057.
HOGENOMiHOG000071678.
KOiK00134.
OMAiFVRVLSW.
OrthoDBiEOG66TG3S.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.
BioCyciSAUR282458:GJA5-831-MONOMER.
BRENDAi1.2.1.12. 3352.

Miscellaneous databases

EvolutionaryTraceiQ6GIL8.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MRSA252.

Entry informationi

Entry nameiG3P1_STAAR
AccessioniPrimary (citable) accession number: Q6GIL8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: July 19, 2004
Last modified: June 24, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.