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Protein

Lipoyl synthase

Gene

lipA

Organism
Staphylococcus aureus (strain MRSA252)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.

Catalytic activityi

Protein N6-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = protein N6-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.

Cofactori

[4Fe-4S] clusterNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]. This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi41Iron-sulfur 1 (4Fe-4S)1
Metal bindingi46Iron-sulfur 1 (4Fe-4S)1
Metal bindingi52Iron-sulfur 1 (4Fe-4S)1
Metal bindingi68Iron-sulfur 2 (4Fe-4S-S-AdoMet)1
Metal bindingi72Iron-sulfur 2 (4Fe-4S-S-AdoMet)1
Metal bindingi75Iron-sulfur 2 (4Fe-4S-S-AdoMet)1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:SAR0887
OrganismiStaphylococcus aureus (strain MRSA252)
Taxonomic identifieri282458 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

  • Cytoplasm

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001023601 – 305Lipoyl synthaseAdd BLAST305

Structurei

3D structure databases

ProteinModelPortaliQ6GIG3.
SMRiQ6GIG3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.

Phylogenomic databases

HOGENOMiHOG000235998.
KOiK03644.
OMAiPYCDIDF.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR031691. LIAS_N.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
PfamiView protein in Pfam
PF16881. LIAS_N. 1 hit.
PF04055. Radical_SAM. 1 hit.
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SFLDiSFLDG01058. lipoyl_synthase_like. 1 hit.
SFLDS00029. Radical_SAM. 1 hit.
SMARTiView protein in SMART
SM00729. Elp3. 1 hit.
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6GIG3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATKNEEILR KPDWLKIKLN TNENYTGLKK MMREKNLNTV CEEAKCPNIH
60 70 80 90 100
ECWGARRTAT FMILGAVCTR ACRFCAVKTG LPNELDLNEP ERVAESVELM
110 120 130 140 150
NLKHVVITAV ARDDLRDAGS NVYAETVRKV RERNPFTTIE ILPSDMGGDY
160 170 180 190 200
DALETLMASR PDILNHNIET VRRLTPRVRA RATYDRTLEF LRRSKELQPD
210 220 230 240 250
IPTKSSIMVG LGETIEEIYE TMDDLRANDV DILTIGQYLQ PSRKHLKVQK
260 270 280 290 300
YYTPLEFGKL RKVAMDKGFK HCQAGPLVRS SYHADEQVNE AAKEKQRQGE

AQLNS
Length:305
Mass (Da):34,885
Last modified:July 19, 2004 - v1
Checksum:i0911CD221177702B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571856 Genomic DNA. Translation: CAG39893.1.
RefSeqiWP_000201875.1. NC_002952.2.

Genome annotation databases

EnsemblBacteriaiCAG39893; CAG39893; SAR0887.
GeneIDi31213676.
KEGGisar:SAR0887.

Similar proteinsi

Entry informationi

Entry nameiLIPA_STAAR
AccessioniPrimary (citable) accession number: Q6GIG3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: July 19, 2004
Last modified: October 25, 2017
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families