Q6GI88 (SPX_STAAR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Regulatory protein spx

Gene names

Name:	spxA
Ordered Locus Names:	SAR0965

Organism

Staphylococcus aureus (strain MRSA252) [Complete proteome] [HAMAP]

Taxonomic identifier

282458 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	131 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Interferes with activator-stimulated transcription by interaction with the RNA polymerase alpha-CTD. May function to globally reduce transcription of genes involved in growth- and development-promoting processes and to increase transcription of genes involved in thiol homeostasis, during periods of extreme stress By similarity. HAMAP MF_01132
Subcellular location	Cytoplasm Probable HAMAP MF_01132.
Sequence similarities	Belongs to the ArsC family. Spx subfamily.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm
Domain	Redox-active center
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 131	131	Regulatory protein spx HAMAP MF_01132		PRO_0000162565
Amino acid modifications
	Disulfide bond	10 ↔ 13		Redox-active Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6GI88 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 89F67A54666699BF	FASTA	131	15,441
	10 20 30 40 50 60 MVTLFTSPSC TSCRKAKAWL QEHDIPYTER NIFSEHLTID EIKQILKMTE DGTDEIISTR 70 80 90 100 110 120 SKTYQKLNVD IDSLPLQDLY SIIQDNPGLL RRPIILDNKR LQVGYNEDEI RRFLPRKVRT 130 FQLQEAQRMV D

References

[1]

"Complete genomes of two clinical Staphylococcus aureus strains: evidence for the rapid evolution of virulence and drug resistance." Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J., Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A., Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C., Clark L., Corton C. , Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z., Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N., Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M., Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004) [PubMed: 15213324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MRSA252.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571856 Genomic DNA. Translation: CAG39970.1.
RefSeq	YP_040384.1. NC_002952.2.
3D structure databases
ProteinModelPortal	Q6GI88.
SMR	Q6GI88. Positions 1-118.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6GI88.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000020409; EBSTAP00000019745; EBSTAG00000020408.
GeneID	2859436.
GenomeReviews	Gene locus SAR0965 in contig BX571856_GR.
KEGG	sar:SAR0965.
PATRIC	19545465. VBIStaAur71814_0965.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1393.
GeneTree	EBGT00050000024542.
HOGENOM	HBG734370.
OMA	FTERNIF.
ProtClustDB	PRK01655.
Enzyme and pathway databases
BioCyc	SAUR282458:SAR0965-MONOMER.
Family and domain databases
HAMAP	MF_01132. SpxA. [Tree]
InterPro	IPR006660. Arsenate_reductase-like. IPR012336. Thioredoxin-like_fold. IPR023731. Tscrpt_reg_Spx. IPR006504. Tscrpt_reg_Spx/MgsR. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
KO	K00537.
Pfam	PF03960. ArsC. 1 hit. [Graphical view]
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
TIGRFAMs	TIGR01617. ArsC_related. 1 hit.
PROSITE	PS51353. ARSC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPX_STAAR

Accession

Primary (citable) accession number: Q6GI88

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	July 19, 2004
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program