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Protein

Enoyl-[acyl-carrier-protein] reductase [NADPH] FabI

Gene

fabI

Organism
Staphylococcus aureus (strain MRSA252)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism (By similarity).By similarity

Catalytic activityi

An acyl-[acyl-carrier protein] + NADP+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH.

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13NADP; via carbonyl oxygen1 Publication1
Sitei40Critical for cofactor specificityBy similarity1
Sitei41Critical for cofactor specificityBy similarity1
Binding sitei94NADP; via carbonyl oxygen1 Publication1
Binding sitei97Substrate; via amide nitrogen and carbonyl oxygenBy similarity1
Active sitei147Proton acceptorBy similarity1
Active sitei157Proton acceptorBy similarity1
Binding sitei164NADP1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi19 – 20NADP1 Publication2
Nucleotide bindingi40 – 44NADP1 Publication5
Nucleotide bindingi66 – 67NADP1 Publication2
Nucleotide bindingi193 – 197NADP1 Publication5

GO - Molecular functioni

GO - Biological processi

  • fatty acid elongation Source: UniProtKB
  • protein homotetramerization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BRENDAi1.3.1.39. 3352.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADPH] FabI (EC:1.3.1.39)
Short name:
ENR
Alternative name(s):
NADPH-dependent enoyl-ACP reductase
Gene namesi
Name:fabI
Ordered Locus Names:SAR0978
OrganismiStaphylococcus aureus (strain MRSA252)
Taxonomic identifieri282458 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3994.
DrugBankiDB08604. Triclosan.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004079791 – 256Enoyl-[acyl-carrier-protein] reductase [NADPH] FabIAdd BLAST256

Proteomic databases

PRIDEiQ6GI75.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Chemistry databases

BindingDBiQ6GI75.

Structurei

Secondary structure

1256
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 12Combined sources5
Beta strandi16 – 18Combined sources3
Helixi20 – 30Combined sources11
Beta strandi34 – 41Combined sources8
Helixi42 – 44Combined sources3
Helixi45 – 52Combined sources8
Helixi53 – 55Combined sources3
Beta strandi61 – 64Combined sources4
Helixi70 – 84Combined sources15
Beta strandi88 – 92Combined sources5
Helixi99 – 102Combined sources4
Helixi106 – 108Combined sources3
Helixi111 – 121Combined sources11
Helixi123 – 133Combined sources11
Beta strandi140 – 146Combined sources7
Helixi148 – 150Combined sources3
Turni155 – 157Combined sources3
Helixi158 – 178Combined sources21
Helixi179 – 181Combined sources3
Beta strandi183 – 190Combined sources8
Helixi196 – 198Combined sources3
Helixi204 – 214Combined sources11
Helixi223 – 234Combined sources12
Helixi236 – 238Combined sources3
Beta strandi245 – 249Combined sources5
Helixi252 – 254Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GNSX-ray2.71A1-256[»]
3GNTX-ray2.75A/B1-256[»]
3GR6X-ray2.28A/D/G/J1-256[»]
4FS3X-ray1.80A1-256[»]
ProteinModelPortaliQ6GI75.
SMRiQ6GI75.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6GI75.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK00208.
OMAiSACKREG.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PTHR24322:SF317. PTHR24322:SF317. 2 hits.
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6GI75-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNLENKTYV IMGIANKRSI AFGVAKVLDQ LGAKLVFTYR KERSRKELEK
60 70 80 90 100
LLEQLNQPEA HLYQIDVQSD EEVINGFEQI GKDVGNIDGV YHSIAFANME
110 120 130 140 150
DLRGRFSETS REGFLLAQDI SSYSLTIVAH EAKKLMPEGG SIVATTYLGG
160 170 180 190 200
EFAVQNYNVM GVAKASLEAN VKYLALDLGP DNIRVNAISA GPIRTLSAKG
210 220 230 240 250
VGGFNTILKE IEERAPLKRN VDQVEVGKTA AYLLSDLSSG VTGENIHVDS

GFHAIK
Length:256
Mass (Da):27,992
Last modified:July 19, 2004 - v1
Checksum:i69487F3F410B9A15
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571856 Genomic DNA. Translation: CAG39983.1.
RefSeqiWP_000933195.1. NC_002952.2.

Genome annotation databases

EnsemblBacteriaiCAG39983; CAG39983; SAR0978.
GeneIDi28381446.
KEGGisar:SAR0978.
PATRICi19545491. VBIStaAur71814_0978.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571856 Genomic DNA. Translation: CAG39983.1.
RefSeqiWP_000933195.1. NC_002952.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GNSX-ray2.71A1-256[»]
3GNTX-ray2.75A/B1-256[»]
3GR6X-ray2.28A/D/G/J1-256[»]
4FS3X-ray1.80A1-256[»]
ProteinModelPortaliQ6GI75.
SMRiQ6GI75.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiQ6GI75.
ChEMBLiCHEMBL3994.
DrugBankiDB08604. Triclosan.

Proteomic databases

PRIDEiQ6GI75.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAG39983; CAG39983; SAR0978.
GeneIDi28381446.
KEGGisar:SAR0978.
PATRICi19545491. VBIStaAur71814_0978.

Phylogenomic databases

KOiK00208.
OMAiSACKREG.

Enzyme and pathway databases

UniPathwayiUPA00094.
BRENDAi1.3.1.39. 3352.

Miscellaneous databases

EvolutionaryTraceiQ6GI75.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PTHR24322:SF317. PTHR24322:SF317. 2 hits.
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFABI_STAAR
AccessioniPrimary (citable) accession number: Q6GI75
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: July 19, 2004
Last modified: November 2, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.