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Protein

Enoyl-[acyl-carrier-protein] reductase [NADPH] FabI

Gene

fabI

Organism
Staphylococcus aureus (strain MRSA252)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism (By similarity).By similarity

Catalytic activityi

An acyl-[acyl-carrier protein] + NADP+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH.

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13NADP; via carbonyl oxygen1 Publication1
Sitei40Critical for cofactor specificityBy similarity1
Sitei41Critical for cofactor specificityBy similarity1
Binding sitei94NADP; via carbonyl oxygen1 Publication1
Binding sitei97Substrate; via amide nitrogen and carbonyl oxygenBy similarity1
Active sitei147Proton acceptorBy similarity1
Active sitei157Proton acceptorBy similarity1
Binding sitei164NADP1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi19 – 20NADP1 Publication2
Nucleotide bindingi40 – 44NADP1 Publication5
Nucleotide bindingi66 – 67NADP1 Publication2
Nucleotide bindingi193 – 197NADP1 Publication5

GO - Molecular functioni

GO - Biological processi

  • fatty acid elongation Source: UniProtKB
  • protein homotetramerization Source: UniProtKB

Keywordsi

Molecular functionOxidoreductase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism
LigandNAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BRENDAi1.3.1.39 3352
UniPathwayiUPA00094

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADPH] FabI (EC:1.3.1.39)
Short name:
ENR
Alternative name(s):
NADPH-dependent enoyl-ACP reductase
Gene namesi
Name:fabI
Ordered Locus Names:SAR0978
OrganismiStaphylococcus aureus (strain MRSA252)
Taxonomic identifieri282458 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3994
DrugBankiDB08604 Triclosan

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004079791 – 256Enoyl-[acyl-carrier-protein] reductase [NADPH] FabIAdd BLAST256

Proteomic databases

PRIDEiQ6GI75

Interactioni

Subunit structurei

Homotetramer.1 Publication

Chemistry databases

BindingDBiQ6GI75

Structurei

Secondary structure

1256
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 12Combined sources5
Beta strandi16 – 18Combined sources3
Helixi20 – 30Combined sources11
Beta strandi34 – 41Combined sources8
Helixi42 – 44Combined sources3
Helixi45 – 52Combined sources8
Helixi53 – 55Combined sources3
Beta strandi61 – 64Combined sources4
Helixi70 – 84Combined sources15
Beta strandi88 – 92Combined sources5
Helixi99 – 102Combined sources4
Helixi106 – 108Combined sources3
Helixi111 – 121Combined sources11
Helixi123 – 133Combined sources11
Beta strandi140 – 146Combined sources7
Helixi148 – 150Combined sources3
Turni155 – 157Combined sources3
Helixi158 – 178Combined sources21
Helixi179 – 181Combined sources3
Beta strandi183 – 190Combined sources8
Helixi196 – 198Combined sources3
Helixi204 – 214Combined sources11
Helixi223 – 234Combined sources12
Helixi236 – 238Combined sources3
Beta strandi245 – 249Combined sources5
Helixi252 – 254Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GNSX-ray2.71A1-256[»]
3GNTX-ray2.75A/B1-256[»]
3GR6X-ray2.28A/D/G/J1-256[»]
4FS3X-ray1.80A1-256[»]
ProteinModelPortaliQ6GI75
SMRiQ6GI75
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6GI75

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK00208
OMAiGILDMIH

Family and domain databases

InterProiView protein in InterPro
IPR014358 Enoyl-ACP_Rdtase_NADH
IPR036291 NAD(P)-bd_dom_sf
IPR002347 SDR_fam
PANTHERiPTHR43159:SF2 PTHR43159:SF2, 1 hit
PIRSFiPIRSF000094 Enoyl-ACP_rdct, 1 hit
PRINTSiPR00081 GDHRDH
SUPFAMiSSF51735 SSF51735, 1 hit

Sequencei

Sequence statusi: Complete.

Q6GI75-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNLENKTYV IMGIANKRSI AFGVAKVLDQ LGAKLVFTYR KERSRKELEK
60 70 80 90 100
LLEQLNQPEA HLYQIDVQSD EEVINGFEQI GKDVGNIDGV YHSIAFANME
110 120 130 140 150
DLRGRFSETS REGFLLAQDI SSYSLTIVAH EAKKLMPEGG SIVATTYLGG
160 170 180 190 200
EFAVQNYNVM GVAKASLEAN VKYLALDLGP DNIRVNAISA GPIRTLSAKG
210 220 230 240 250
VGGFNTILKE IEERAPLKRN VDQVEVGKTA AYLLSDLSSG VTGENIHVDS

GFHAIK
Length:256
Mass (Da):27,992
Last modified:July 19, 2004 - v1
Checksum:i69487F3F410B9A15
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571856 Genomic DNA Translation: CAG39983.1
RefSeqiWP_000933195.1, NC_002952.2

Genome annotation databases

EnsemblBacteriaiCAG39983; CAG39983; SAR0978
KEGGisar:SAR0978

Similar proteinsi

Entry informationi

Entry nameiFABI_STAAR
AccessioniPrimary (citable) accession number: Q6GI75
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: July 19, 2004
Last modified: March 28, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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