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Q6GI31

- ATL_STAAR

UniProt

Q6GI31 - ATL_STAAR

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Protein
Bifunctional autolysin
Gene
atl, nag, SAR1026
Organism
Staphylococcus aureus (strain MRSA252)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Endohydrolysis of the di-N-acetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -[(Man)5(GlcNAc)2]-Asn structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact. Cleaves the peptidoglycan connecting the daughter cells at the end of the cell division cycle, resulting in the separation of the two newly divided cells. Acts as an autolysin in penicillin-induced lysis By similarity.

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact.

GO - Molecular functioni

  1. N-acetylmuramoyl-L-alanine amidase activity Source: UniProtKB-EC
  2. amidase activity Source: InterPro
  3. mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell wall macromolecule metabolic process Source: InterPro
  2. peptidoglycan catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciSAUR282458:GJA5-1027-MONOMER.

Protein family/group databases

CAZyiGH73. Glycoside Hydrolase Family 73.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional autolysin
Including the following 2 domains:
N-acetylmuramoyl-L-alanine amidase (EC:3.5.1.28)
Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (EC:3.2.1.96)
Gene namesi
Name:atl
Synonyms:nag
Ordered Locus Names:SAR1026
OrganismiStaphylococcus aureus (strain MRSA252)
Taxonomic identifieri282458 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000000596: Chromosome

Subcellular locationi

Secreted By similarity
Note: Secreted, and then anchored on the cell surface at the peripheral cell wall above the completed septum (septal region), for the next cell division cycle By similarity.

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929 Reviewed prediction
Add
BLAST
Chaini30 – 12571228Bifunctional autolysin
PRO_0000045476Add
BLAST

Post-translational modificationi

Undergoes proteolytic processing to generate the two extracellular lytic enzymes, probably at the septal region on the cell surface By similarity.

Interactioni

Subunit structurei

Oligomer; forms a ring structure at the cell surface which is important for efficient partitioning of daughter cells after cell division By similarity.

Protein-protein interaction databases

STRINGi282458.SAR1026.

Structurei

3D structure databases

ProteinModelPortaliQ6GI31.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati423 – 5901681
Add
BLAST
Repeati597 – 7591632
Add
BLAST
Repeati771 – 9331633
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni197 – 776580N-acetylmuramoyl-L-alanine amidase
Add
BLAST
Regioni777 – 1257481Endo-beta-N-acetylglucosaminidase
Add
BLAST

Domaini

The repeat domains R1, R2 and R3 are responsible for directing the proteins to the septal region By similarity.

Sequence similaritiesi

In the N-terminal section; belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.
In the C-terminal section; belongs to the glycosyl hydrolase 73 family.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG4193.
HOGENOMiHOG000279968.
KOiK13714.
OMAiKSGWISK.
OrthoDBiEOG6GXTN7.

Family and domain databases

Gene3Di3.40.80.10. 1 hit.
InterProiIPR002502. Amidase_domain.
IPR013338. Lysozyme_subfam2_dom.
IPR002901. MGlyc_endo_b_GlcNAc_like_dom.
[Graphical view]
PfamiPF01510. Amidase_2. 1 hit.
PF01832. Glucosaminidase. 1 hit.
[Graphical view]
SMARTiSM00644. Ami_2. 1 hit.
SM00047. LYZ2. 1 hit.
[Graphical view]
SUPFAMiSSF55846. SSF55846. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6GI31-1 [UniParc]FASTAAdd to Basket

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MAKKFNYKLP SMVALTLVGS AVTAHQVQAA ETTQDQTTNK NVLDSNKVKA     50
TTEQAKAEVK NPTQNISGTQ VYQDPAIVQP KAANKTGNAQ VNQKVDTTQV 100
NGDTRATQST TSNNAKPVTK STNTTAPKTN NNVTSAGYSL VDDEDDNSEN 150
QINPELIKSA AKPAALETQY KAAAPKATPV APKAKTEATP KVTTFSASAQ 200
PRSAAAAPKT SLPKYKPQVN SSINDYIRKN NLKAPKIEED YTSYFPKYAY 250
RNGVGRPEGI VVHDTANDRS TINGEISYMK NNYQNAFVHA FVDGDRIIET 300
APTDYLSWGV GAVGNPRFIN VEIVHTHDYA SFARSMNNYA DYAATQLQYY 350
GLKPDSAEYD GNGTVWTHYA VSKYLGGTDH ADPHGYLRSH NYSYDQLYDL 400
INEKYLIKMG KVAPWGTQST TTPTTPSKPS TPSKPSTPST GKLTVAANNG 450
VAQIKPTNSG LYTTVYDKTG KATNEVQKTF AVSKTATLGN QKFYLVQDYN 500
SGNKFGWVKE GDVVYNTAKS PVNVNQSYSI KPGTKLYTVP WGTSKQVAGS 550
VSGSGNQTFK ASKQQQIDKS IYLYGSVNGK SGWVSKAYLV DTAKPTPTPT 600
PKPSTPTTNN KLTVSSLNGV AQINAKNNGL FTTVYDKTGK PTKEVQKTFA 650
VTKEASLGGN KFYLVKDYNS PTLIGWVKQG DVIYNNAKSP VNVMQTYTVK 700
PGTKLYSVPW GTYKQEAGAV SGTGNQTFKA TKQQQIDKSI YLYGTVNGKS 750
GWISKAYLAV PAAPKKAVAQ PKTAVKAYAV TKPQTTQTVS KIAQVKPNNT 800
GIRASVYEKT AKNGAKYADR TFYVTKERAH GNETYVLLNN TSHNIPLGWF 850
NVKDLNVQNL GKEVKTTQKY TVNRSNNGLS MVPWGTKNQV ILTGNNIAQG 900
TFNATKQVSV GKDVYLYGTI NNRTGWVNSK DLTAPTAVKP TTSAAKDYNY 950
TYVIKNGNGY YYVTPNSDTA KYSLKAFNEQ PFAVVKEQVI NGQTWYYGKL 1000
SNGKLAWIKS TDLAKELIKY NQIGMTLNQV AQIQAGLQYK PQVQRVPGKW 1050
TDANFNDVKH AMDTKRLAQD PALKYQFLRL DQPQNISIDK INQFLKGKGV 1100
LENQGAAFNK AAQMYGINEV YLISHALLET GNGTSQLAKG ADVVNNKVVT 1150
NSNTKYHNVF GIAAYDNDPL REGIKYAKQA GWDTVSKAIV GGAKFIGNSY 1200
VKAGQNTLYK MRWNPAHPGT HQYATDVDWA NINAKIIKGY YDKIGEVGKY 1250
FDIPQYK 1257
Length:1,257
Mass (Da):137,528
Last modified:July 19, 2004 - v1
Checksum:i8CEB3DB932B1720D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX571856 Genomic DNA. Translation: CAG40030.1.
RefSeqiYP_040441.1. NC_002952.2.

Genome annotation databases

EnsemblBacteriaiCAG40030; CAG40030; SAR1026.
GeneIDi2859873.
KEGGisar:SAR1026.
PATRICi19545587. VBIStaAur71814_1024.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX571856 Genomic DNA. Translation: CAG40030.1 .
RefSeqi YP_040441.1. NC_002952.2.

3D structure databases

ProteinModelPortali Q6GI31.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 282458.SAR1026.

Protein family/group databases

CAZyi GH73. Glycoside Hydrolase Family 73.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAG40030 ; CAG40030 ; SAR1026 .
GeneIDi 2859873.
KEGGi sar:SAR1026.
PATRICi 19545587. VBIStaAur71814_1024.

Phylogenomic databases

eggNOGi COG4193.
HOGENOMi HOG000279968.
KOi K13714.
OMAi KSGWISK.
OrthoDBi EOG6GXTN7.

Enzyme and pathway databases

BioCyci SAUR282458:GJA5-1027-MONOMER.

Family and domain databases

Gene3Di 3.40.80.10. 1 hit.
InterProi IPR002502. Amidase_domain.
IPR013338. Lysozyme_subfam2_dom.
IPR002901. MGlyc_endo_b_GlcNAc_like_dom.
[Graphical view ]
Pfami PF01510. Amidase_2. 1 hit.
PF01832. Glucosaminidase. 1 hit.
[Graphical view ]
SMARTi SM00644. Ami_2. 1 hit.
SM00047. LYZ2. 1 hit.
[Graphical view ]
SUPFAMi SSF55846. SSF55846. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MRSA252.

Entry informationi

Entry nameiATL_STAAR
AccessioniPrimary (citable) accession number: Q6GI31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: July 19, 2004
Last modified: September 3, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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