Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q6GI31

- ATL_STAAR

UniProt

Q6GI31 - ATL_STAAR

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Bifunctional autolysin

Gene

atl

Organism
Staphylococcus aureus (strain MRSA252)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Endohydrolysis of the di-N-acetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -[(Man)5(GlcNAc)2]-Asn structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact. Cleaves the peptidoglycan connecting the daughter cells at the end of the cell division cycle, resulting in the separation of the two newly divided cells. Acts as an autolysin in penicillin-induced lysis (By similarity).By similarity

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact.

GO - Molecular functioni

  1. amidase activity Source: InterPro
  2. mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity Source: UniProtKB-EC
  3. N-acetylmuramoyl-L-alanine amidase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell wall organization Source: UniProtKB-KW
  2. peptidoglycan catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciSAUR282458:GJA5-1027-MONOMER.

Protein family/group databases

CAZyiGH73. Glycoside Hydrolase Family 73.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional autolysin
Including the following 2 domains:
N-acetylmuramoyl-L-alanine amidase (EC:3.5.1.28)
Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (EC:3.2.1.96)
Gene namesi
Name:atl
Synonyms:nag
Ordered Locus Names:SAR1026
OrganismiStaphylococcus aureus (strain MRSA252)
Taxonomic identifieri282458 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000000596: Chromosome

Subcellular locationi

Secreted By similarity
Note: Secreted, and then anchored on the cell surface at the peripheral cell wall above the completed septum (septal region), for the next cell division cycle.By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence AnalysisAdd
BLAST
Chaini30 – 12571228Bifunctional autolysinPRO_0000045476Add
BLAST

Post-translational modificationi

Undergoes proteolytic processing to generate the two extracellular lytic enzymes, probably at the septal region on the cell surface.By similarity

Interactioni

Subunit structurei

Oligomer; forms a ring structure at the cell surface which is important for efficient partitioning of daughter cells after cell division.By similarity

Protein-protein interaction databases

STRINGi282458.SAR1026.

Structurei

3D structure databases

ProteinModelPortaliQ6GI31.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati423 – 5901681Add
BLAST
Repeati597 – 7591632Add
BLAST
Repeati771 – 9331633Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni197 – 776580N-acetylmuramoyl-L-alanine amidaseAdd
BLAST
Regioni777 – 1257481Endo-beta-N-acetylglucosaminidaseAdd
BLAST

Domaini

The repeat domains R1, R2 and R3 are responsible for directing the proteins to the septal region.By similarity

Sequence similaritiesi

In the N-terminal section; belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.Curated
In the C-terminal section; belongs to the glycosyl hydrolase 73 family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG4193.
HOGENOMiHOG000279968.
KOiK13714.
OMAiKSGWISK.
OrthoDBiEOG6GXTN7.

Family and domain databases

Gene3Di3.40.80.10. 1 hit.
InterProiIPR002502. Amidase_domain.
IPR013338. Lysozyme_subfam2_dom.
IPR002901. MGlyc_endo_b_GlcNAc_like_dom.
[Graphical view]
PfamiPF01510. Amidase_2. 1 hit.
PF01832. Glucosaminidase. 1 hit.
[Graphical view]
SMARTiSM00644. Ami_2. 1 hit.
SM00047. LYZ2. 1 hit.
[Graphical view]
SUPFAMiSSF55846. SSF55846. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6GI31-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKKFNYKLP SMVALTLVGS AVTAHQVQAA ETTQDQTTNK NVLDSNKVKA
60 70 80 90 100
TTEQAKAEVK NPTQNISGTQ VYQDPAIVQP KAANKTGNAQ VNQKVDTTQV
110 120 130 140 150
NGDTRATQST TSNNAKPVTK STNTTAPKTN NNVTSAGYSL VDDEDDNSEN
160 170 180 190 200
QINPELIKSA AKPAALETQY KAAAPKATPV APKAKTEATP KVTTFSASAQ
210 220 230 240 250
PRSAAAAPKT SLPKYKPQVN SSINDYIRKN NLKAPKIEED YTSYFPKYAY
260 270 280 290 300
RNGVGRPEGI VVHDTANDRS TINGEISYMK NNYQNAFVHA FVDGDRIIET
310 320 330 340 350
APTDYLSWGV GAVGNPRFIN VEIVHTHDYA SFARSMNNYA DYAATQLQYY
360 370 380 390 400
GLKPDSAEYD GNGTVWTHYA VSKYLGGTDH ADPHGYLRSH NYSYDQLYDL
410 420 430 440 450
INEKYLIKMG KVAPWGTQST TTPTTPSKPS TPSKPSTPST GKLTVAANNG
460 470 480 490 500
VAQIKPTNSG LYTTVYDKTG KATNEVQKTF AVSKTATLGN QKFYLVQDYN
510 520 530 540 550
SGNKFGWVKE GDVVYNTAKS PVNVNQSYSI KPGTKLYTVP WGTSKQVAGS
560 570 580 590 600
VSGSGNQTFK ASKQQQIDKS IYLYGSVNGK SGWVSKAYLV DTAKPTPTPT
610 620 630 640 650
PKPSTPTTNN KLTVSSLNGV AQINAKNNGL FTTVYDKTGK PTKEVQKTFA
660 670 680 690 700
VTKEASLGGN KFYLVKDYNS PTLIGWVKQG DVIYNNAKSP VNVMQTYTVK
710 720 730 740 750
PGTKLYSVPW GTYKQEAGAV SGTGNQTFKA TKQQQIDKSI YLYGTVNGKS
760 770 780 790 800
GWISKAYLAV PAAPKKAVAQ PKTAVKAYAV TKPQTTQTVS KIAQVKPNNT
810 820 830 840 850
GIRASVYEKT AKNGAKYADR TFYVTKERAH GNETYVLLNN TSHNIPLGWF
860 870 880 890 900
NVKDLNVQNL GKEVKTTQKY TVNRSNNGLS MVPWGTKNQV ILTGNNIAQG
910 920 930 940 950
TFNATKQVSV GKDVYLYGTI NNRTGWVNSK DLTAPTAVKP TTSAAKDYNY
960 970 980 990 1000
TYVIKNGNGY YYVTPNSDTA KYSLKAFNEQ PFAVVKEQVI NGQTWYYGKL
1010 1020 1030 1040 1050
SNGKLAWIKS TDLAKELIKY NQIGMTLNQV AQIQAGLQYK PQVQRVPGKW
1060 1070 1080 1090 1100
TDANFNDVKH AMDTKRLAQD PALKYQFLRL DQPQNISIDK INQFLKGKGV
1110 1120 1130 1140 1150
LENQGAAFNK AAQMYGINEV YLISHALLET GNGTSQLAKG ADVVNNKVVT
1160 1170 1180 1190 1200
NSNTKYHNVF GIAAYDNDPL REGIKYAKQA GWDTVSKAIV GGAKFIGNSY
1210 1220 1230 1240 1250
VKAGQNTLYK MRWNPAHPGT HQYATDVDWA NINAKIIKGY YDKIGEVGKY

FDIPQYK
Length:1,257
Mass (Da):137,528
Last modified:July 19, 2004 - v1
Checksum:i8CEB3DB932B1720D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX571856 Genomic DNA. Translation: CAG40030.1.
RefSeqiYP_040441.1. NC_002952.2.

Genome annotation databases

EnsemblBacteriaiCAG40030; CAG40030; SAR1026.
GeneIDi2859873.
KEGGisar:SAR1026.
PATRICi19545587. VBIStaAur71814_1024.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX571856 Genomic DNA. Translation: CAG40030.1 .
RefSeqi YP_040441.1. NC_002952.2.

3D structure databases

ProteinModelPortali Q6GI31.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 282458.SAR1026.

Protein family/group databases

CAZyi GH73. Glycoside Hydrolase Family 73.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAG40030 ; CAG40030 ; SAR1026 .
GeneIDi 2859873.
KEGGi sar:SAR1026.
PATRICi 19545587. VBIStaAur71814_1024.

Phylogenomic databases

eggNOGi COG4193.
HOGENOMi HOG000279968.
KOi K13714.
OMAi KSGWISK.
OrthoDBi EOG6GXTN7.

Enzyme and pathway databases

BioCyci SAUR282458:GJA5-1027-MONOMER.

Family and domain databases

Gene3Di 3.40.80.10. 1 hit.
InterProi IPR002502. Amidase_domain.
IPR013338. Lysozyme_subfam2_dom.
IPR002901. MGlyc_endo_b_GlcNAc_like_dom.
[Graphical view ]
Pfami PF01510. Amidase_2. 1 hit.
PF01832. Glucosaminidase. 1 hit.
[Graphical view ]
SMARTi SM00644. Ami_2. 1 hit.
SM00047. LYZ2. 1 hit.
[Graphical view ]
SUPFAMi SSF55846. SSF55846. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MRSA252.

Entry informationi

Entry nameiATL_STAAR
AccessioniPrimary (citable) accession number: Q6GI31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: July 19, 2004
Last modified: October 29, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3