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Q6GI31

- ATL_STAAR

UniProt

Q6GI31 - ATL_STAAR

Protein

Bifunctional autolysin

Gene

atl

Organism
Staphylococcus aureus (strain MRSA252)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (19 Jul 2004)
      Previous versions | rss
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    Functioni

    Endohydrolysis of the di-N-acetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -[(Man)5(GlcNAc)2]-Asn structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact. Cleaves the peptidoglycan connecting the daughter cells at the end of the cell division cycle, resulting in the separation of the two newly divided cells. Acts as an autolysin in penicillin-induced lysis By similarity.By similarity

    Catalytic activityi

    Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
    Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact.

    GO - Molecular functioni

    1. amidase activity Source: InterPro
    2. mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity Source: UniProtKB-EC
    3. N-acetylmuramoyl-L-alanine amidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cell wall macromolecule metabolic process Source: InterPro
    2. peptidoglycan catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciSAUR282458:GJA5-1027-MONOMER.

    Protein family/group databases

    CAZyiGH73. Glycoside Hydrolase Family 73.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional autolysin
    Including the following 2 domains:
    N-acetylmuramoyl-L-alanine amidase (EC:3.5.1.28)
    Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (EC:3.2.1.96)
    Gene namesi
    Name:atl
    Synonyms:nag
    Ordered Locus Names:SAR1026
    OrganismiStaphylococcus aureus (strain MRSA252)
    Taxonomic identifieri282458 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000000596: Chromosome

    Subcellular locationi

    Secreted By similarity
    Note: Secreted, and then anchored on the cell surface at the peripheral cell wall above the completed septum (septal region), for the next cell division cycle.By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Sequence AnalysisAdd
    BLAST
    Chaini30 – 12571228Bifunctional autolysinPRO_0000045476Add
    BLAST

    Post-translational modificationi

    Undergoes proteolytic processing to generate the two extracellular lytic enzymes, probably at the septal region on the cell surface.By similarity

    Interactioni

    Subunit structurei

    Oligomer; forms a ring structure at the cell surface which is important for efficient partitioning of daughter cells after cell division.By similarity

    Protein-protein interaction databases

    STRINGi282458.SAR1026.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6GI31.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati423 – 5901681Add
    BLAST
    Repeati597 – 7591632Add
    BLAST
    Repeati771 – 9331633Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni197 – 776580N-acetylmuramoyl-L-alanine amidaseAdd
    BLAST
    Regioni777 – 1257481Endo-beta-N-acetylglucosaminidaseAdd
    BLAST

    Domaini

    The repeat domains R1, R2 and R3 are responsible for directing the proteins to the septal region.By similarity

    Sequence similaritiesi

    In the N-terminal section; belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.Curated
    In the C-terminal section; belongs to the glycosyl hydrolase 73 family.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG4193.
    HOGENOMiHOG000279968.
    KOiK13714.
    OMAiKSGWISK.
    OrthoDBiEOG6GXTN7.

    Family and domain databases

    Gene3Di3.40.80.10. 1 hit.
    InterProiIPR002502. Amidase_domain.
    IPR013338. Lysozyme_subfam2_dom.
    IPR002901. MGlyc_endo_b_GlcNAc_like_dom.
    [Graphical view]
    PfamiPF01510. Amidase_2. 1 hit.
    PF01832. Glucosaminidase. 1 hit.
    [Graphical view]
    SMARTiSM00644. Ami_2. 1 hit.
    SM00047. LYZ2. 1 hit.
    [Graphical view]
    SUPFAMiSSF55846. SSF55846. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q6GI31-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKKFNYKLP SMVALTLVGS AVTAHQVQAA ETTQDQTTNK NVLDSNKVKA     50
    TTEQAKAEVK NPTQNISGTQ VYQDPAIVQP KAANKTGNAQ VNQKVDTTQV 100
    NGDTRATQST TSNNAKPVTK STNTTAPKTN NNVTSAGYSL VDDEDDNSEN 150
    QINPELIKSA AKPAALETQY KAAAPKATPV APKAKTEATP KVTTFSASAQ 200
    PRSAAAAPKT SLPKYKPQVN SSINDYIRKN NLKAPKIEED YTSYFPKYAY 250
    RNGVGRPEGI VVHDTANDRS TINGEISYMK NNYQNAFVHA FVDGDRIIET 300
    APTDYLSWGV GAVGNPRFIN VEIVHTHDYA SFARSMNNYA DYAATQLQYY 350
    GLKPDSAEYD GNGTVWTHYA VSKYLGGTDH ADPHGYLRSH NYSYDQLYDL 400
    INEKYLIKMG KVAPWGTQST TTPTTPSKPS TPSKPSTPST GKLTVAANNG 450
    VAQIKPTNSG LYTTVYDKTG KATNEVQKTF AVSKTATLGN QKFYLVQDYN 500
    SGNKFGWVKE GDVVYNTAKS PVNVNQSYSI KPGTKLYTVP WGTSKQVAGS 550
    VSGSGNQTFK ASKQQQIDKS IYLYGSVNGK SGWVSKAYLV DTAKPTPTPT 600
    PKPSTPTTNN KLTVSSLNGV AQINAKNNGL FTTVYDKTGK PTKEVQKTFA 650
    VTKEASLGGN KFYLVKDYNS PTLIGWVKQG DVIYNNAKSP VNVMQTYTVK 700
    PGTKLYSVPW GTYKQEAGAV SGTGNQTFKA TKQQQIDKSI YLYGTVNGKS 750
    GWISKAYLAV PAAPKKAVAQ PKTAVKAYAV TKPQTTQTVS KIAQVKPNNT 800
    GIRASVYEKT AKNGAKYADR TFYVTKERAH GNETYVLLNN TSHNIPLGWF 850
    NVKDLNVQNL GKEVKTTQKY TVNRSNNGLS MVPWGTKNQV ILTGNNIAQG 900
    TFNATKQVSV GKDVYLYGTI NNRTGWVNSK DLTAPTAVKP TTSAAKDYNY 950
    TYVIKNGNGY YYVTPNSDTA KYSLKAFNEQ PFAVVKEQVI NGQTWYYGKL 1000
    SNGKLAWIKS TDLAKELIKY NQIGMTLNQV AQIQAGLQYK PQVQRVPGKW 1050
    TDANFNDVKH AMDTKRLAQD PALKYQFLRL DQPQNISIDK INQFLKGKGV 1100
    LENQGAAFNK AAQMYGINEV YLISHALLET GNGTSQLAKG ADVVNNKVVT 1150
    NSNTKYHNVF GIAAYDNDPL REGIKYAKQA GWDTVSKAIV GGAKFIGNSY 1200
    VKAGQNTLYK MRWNPAHPGT HQYATDVDWA NINAKIIKGY YDKIGEVGKY 1250
    FDIPQYK 1257
    Length:1,257
    Mass (Da):137,528
    Last modified:July 19, 2004 - v1
    Checksum:i8CEB3DB932B1720D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX571856 Genomic DNA. Translation: CAG40030.1.
    RefSeqiYP_040441.1. NC_002952.2.

    Genome annotation databases

    EnsemblBacteriaiCAG40030; CAG40030; SAR1026.
    GeneIDi2859873.
    KEGGisar:SAR1026.
    PATRICi19545587. VBIStaAur71814_1024.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX571856 Genomic DNA. Translation: CAG40030.1 .
    RefSeqi YP_040441.1. NC_002952.2.

    3D structure databases

    ProteinModelPortali Q6GI31.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 282458.SAR1026.

    Protein family/group databases

    CAZyi GH73. Glycoside Hydrolase Family 73.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAG40030 ; CAG40030 ; SAR1026 .
    GeneIDi 2859873.
    KEGGi sar:SAR1026.
    PATRICi 19545587. VBIStaAur71814_1024.

    Phylogenomic databases

    eggNOGi COG4193.
    HOGENOMi HOG000279968.
    KOi K13714.
    OMAi KSGWISK.
    OrthoDBi EOG6GXTN7.

    Enzyme and pathway databases

    BioCyci SAUR282458:GJA5-1027-MONOMER.

    Family and domain databases

    Gene3Di 3.40.80.10. 1 hit.
    InterProi IPR002502. Amidase_domain.
    IPR013338. Lysozyme_subfam2_dom.
    IPR002901. MGlyc_endo_b_GlcNAc_like_dom.
    [Graphical view ]
    Pfami PF01510. Amidase_2. 1 hit.
    PF01832. Glucosaminidase. 1 hit.
    [Graphical view ]
    SMARTi SM00644. Ami_2. 1 hit.
    SM00047. LYZ2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55846. SSF55846. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MRSA252.

    Entry informationi

    Entry nameiATL_STAAR
    AccessioniPrimary (citable) accession number: Q6GI31
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 10, 2006
    Last sequence update: July 19, 2004
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3