Reviewed, UniProtKB/Swiss-Prot Q6GI19 (PURK_STAAR)

Names and origin

Protein names

Recommended name:
    Phosphoribosylaminoimidazole carboxylase ATPase subunit
    EC=4.1.1.21
Alternative name(s):
    AIR carboxylase
      Short name=AIRC

Gene names

Name:	purK
Ordered Locus Names:	SAR1039

Organism

Staphylococcus aureus (strain MRSA252) [Complete proteome] [HAMAP]

Taxonomic identifier

282458 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Possesses an ATPase activity that is dependent on the presence of AIR (aminoimidazole ribonucleotide). The association of purK and purE produces an enzyme complex capable of converting AIR to CAIR efficiently under physiological condition By similarity.
Catalytic activity	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the purK/purT family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: InterPro pentose-phosphate shunt Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphogluconate dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: InterPro phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	Phosphoribosylaminoimidazole carboxylase ATPase subunit		PRO_0000075006
Regions
	Domain	112 – 296	185	ATP-grasp
	Nucleotide binding	183 – 186	4	ATP By similarity
	Nucleotide binding	266 – 267	2	ATP By similarity
Sites
	Binding site	108	1	ATP By similarity
	Binding site	148	1	ATP By similarity
	Binding site	159	1	ATP By similarity
	Binding site	191	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6GI19-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 5AADFD6A4646BE14	FASTA	374	42,470
	10 20 30 40 50 60 MNFNKLKFGA TIGIIGGGQL GKMMAQSAQK MGYKVAVLDP AEDCPCRYVA HEFIQAKYDD 70 80 90 100 110 120 EKALNQLGQK CDVITYEFEN ISAQQLKLLC EKYNIPQGYQ AIQLLQDRLT EKETLKSAGT 130 140 150 160 170 180 KVVPFISVKE SKDIDKAIET LGYPFIVKTR FGGYDGKGQV LINNEKDLQE GIKLIETSEC 190 200 210 220 230 240 VAEKYLNIKK EVSLTVTRGN NNQITYFPLQ ENEHRNQILF KTIVPARIDK IAEAKEQVNK 250 260 270 280 290 300 IIQSIHFIGT FTVEFFIDSN NQLYVNEIAP RPHNSGHYSI EACDYSQFDT HILAVTGQSL 310 320 330 340 350 360 PNSIELLKPA VMMNLLGKDL DLLENEFNEH PEWHLHIYGK SERKDSRKMG HMTVLTNDVN 370 QTEQDMYAKF EGSN

References

[1]

"Complete genomes of two clinical Staphylococcus aureus strains: evidence for the rapid evolution of virulence and drug resistance." Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J., Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A., Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C., Clark L., Corton C. , Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z., Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N., Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M., Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004) [PubMed: 15213324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571856 Genomic DNA. Translation: CAG40042.1.
RefSeq	YP_040453.1.
3D structure databases
SMR	Q6GI19. Positions 11-368.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6GI19.
Genome annotation databases
GeneID	2859548.
GenomeReviews	Gene locus SAR1039 in contig BX571856_GR.
KEGG	sar:SAR1039.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0026.
HOGENOM	HBG516369.
OMA	YDGKGQA.
Enzyme and pathway databases
BioCyc	SAUR282458:SAR1039-MONOMER.
Family and domain databases
InterPro	IPR006115. 6PGDH_NAD-bd. IPR005875. AIR_COase_ATPase-su. IPR011761. ATP-grasp. IPR003135. ATP-grasp_carboxylate-amine. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
PANTHER	PTHR23047:SF1. PurK_ATP. 1 hit.
Pfam	PF02222. ATP-grasp. 1 hit. PF03446. NAD_binding_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01161. purK. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURK_STAAR

Accession

Primary (citable) accession number: Q6GI19

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	July 19, 2004
Last modified:	February 9, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)