Q6GI12 (PUR3_STAAR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylglycinamide formyltransferase
EC=2.1.2.2

Alternative name(s):
5'-phosphoribosylglycinamide transformylase
GAR transformylase
Short name=GART

Gene names

Name:	purN
Ordered Locus Names:	SAR1046

Organism

Staphylococcus aureus (strain MRSA252) [Complete proteome] [HAMAP]

Taxonomic identifier

282458 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the GART family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	phosphoribosylglycinamide formyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	Phosphoribosylglycinamide formyltransferase		PRO_0000074948
Regions
	Region	12 – 14	3	5'-phosphoribosylglycinamide binding By similarity
	Region	91 – 94	4	10-formyltetrahydrofolate binding By similarity
Sites
	Active site	110	1	Proton donor By similarity
	Binding site	66	1	10-formyltetrahydrofolate By similarity
	Binding site	108	1	10-formyltetrahydrofolate By similarity
	Binding site	146	1	10-formyltetrahydrofolate; via amide nitrogen By similarity
	Binding site	175	1	5'-phosphoribosylglycinamide By similarity
	Site	146	1	Raises pKa of active site His By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6GI12 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: D73644C2591D8DBA	FASTA	188	21,093
	10 20 30 40 50 60 MVKIAIFASG SGSNFENIVE HVESGKLENI EVTALYTDHQ NAFCIDRAKK HDIPVYINEP 70 80 90 100 110 120 KQFGSKAAYE QHLVTLLNED KVEWIILAGY MRLIGPDLLA SFEGKILNIH PSLLPKYKGI 130 140 150 160 170 180 DAIGQAYHSG DTITGSTVHY VDSGMDTGEI IEQRQCDIRP DDSKEQLEEK VKKLEYELYP SVIAKIVK

References

[1]

"Complete genomes of two clinical Staphylococcus aureus strains: evidence for the rapid evolution of virulence and drug resistance." Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J., Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A., Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C., Clark L., Corton C. , Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z., Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N., Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M., Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004) [PubMed: 15213324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MRSA252.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571856 Genomic DNA. Translation: CAG40049.1.
RefSeq	YP_040460.1. NC_002952.2.
3D structure databases
ProteinModelPortal	Q6GI12.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6GI12.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000019854; EBSTAP00000019190; EBSTAG00000019853.
GeneID	2859551.
GenomeReviews	Gene locus SAR1046 in contig BX571856_GR.
KEGG	sar:SAR1046.
PATRIC	19545629. VBIStaAur71814_1045.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0299.
GeneTree	EBGT00050000024715.
HOGENOM	HBG649382.
OMA	GSNMQAI.
PhylomeDB	Q6GI12.
ProtClustDB	CLSK885118.
Enzyme and pathway databases
BioCyc	SAUR282458:SAR1046-MONOMER.
Family and domain databases
InterPro	IPR002376. Formyl_transf_N. IPR004607. PurN_trans. [Graphical view]
Gene3D	G3DSA:3.40.50.170. Formyl_transf_N. 1 hit.
KO	K11175.
Pfam	PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00639. PurN. 1 hit.
PROSITE	PS00373. GART. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR3_STAAR

Accession

Primary (citable) accession number: Q6GI12

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	July 19, 2004
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program