Reviewed, UniProtKB/Swiss-Prot Q6GI10 (PUR2_STAAR)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	SAR1048

Organism

Staphylococcus aureus (strain MRSA252) [Complete proteome] [HAMAP]

Taxonomic identifier

282458 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity. HAMAP MF_00138
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151481
Regions
	Domain	108 – 311	204	ATP-grasp
	Nucleotide binding	134 – 191	58	ATP By similarity
Sites
	Metal binding	281	1	Magnesium or manganese By similarity
	Metal binding	283	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6GI10-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: E09101074D200A1A	FASTA	415	45,849
	10 20 30 40 50 60 MNVLVIGAGG REHALAYKLN QSNLVKQVFV IPGNEAMTPI AEVHTEISES DHQGILDFAK 70 80 90 100 110 120 QQNVDWVVIG PEQPLIDGLA DILRANGFKV FGPNKQAAQI EGSKLFAKKI MEKYNIPTAD 130 140 150 160 170 180 YKEVERKKDA LTYIENCEFP VVVKKDGLAA GKGVIIADTI EAARSAIEIM YGDEEEGTVV 190 200 210 220 230 240 FETFLEGEEF SLMTFVNGEL AVPFDCIAQD HKRAFDHDEG PNTGGMGAYC PVPHISDDVL 250 260 270 280 290 300 KLTNETIAQP IAKAMLNEGY QFFGVLYIGA ILTKDGPKVI EFNARFGDPE AQVLLSRMES 310 320 330 340 350 360 DLMQHIIDLD EGKRTEFKWK NEAIVGVMLA SKGYPDAYEK GHKVSGFDLN ENYFVSGLKK 370 380 390 400 410 QGDTFVTSGG RVILAIGKGD NVHDAQRDAY EKVSQIQSDH LFYRHDIANK ALQLK

References

[1]

"Complete genomes of two clinical Staphylococcus aureus strains: evidence for the rapid evolution of virulence and drug resistance." Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J., Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A., Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C., Clark L., Corton C. , Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z., Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N., Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M., Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004) [PubMed: 15213324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571856 Genomic DNA. Translation: CAG40051.1.
RefSeq	YP_040462.1.
3D structure databases
SMR	Q6GI10. Positions 1-413.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6GI10.
Genome annotation databases
GeneID	2859544.
GenomeReviews	Gene locus SAR1048 in contig BX571856_GR.
KEGG	sar:SAR1048.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0151.
HOGENOM	HBG404060.
OMA	FSLMAFV.
Enzyme and pathway databases
BioCyc	SAUR282458:SAR1048-MONOMER.
Family and domain databases
HAMAP	MF_00138. GARS. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_STAAR

Accession

Primary (citable) accession number: Q6GI10

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	July 19, 2004
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)