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Protein

tRNA (guanine-N(1)-)-methyltransferase

Gene

trmD

Organism
Staphylococcus aureus (strain MRSA252)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates guanosine-37 in various tRNAs.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei111 – 1111S-adenosyl-L-methionine; via amide nitrogenUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSAUR282458:GJA5-1219-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(1)-)-methyltransferaseUniRule annotation (EC:2.1.1.228UniRule annotation)
Alternative name(s):
M1G-methyltransferaseUniRule annotation
tRNA [GM37] methyltransferaseUniRule annotation
Gene namesi
Name:trmDUniRule annotation
Ordered Locus Names:SAR1216
OrganismiStaphylococcus aureus (strain MRSA252)
Taxonomic identifieri282458 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 245245tRNA (guanine-N(1)-)-methyltransferasePRO_0000060457Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

Secondary structure

1
245
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 87Combined sources
Helixi10 – 134Combined sources
Helixi14 – 196Combined sources
Turni20 – 223Combined sources
Helixi23 – 275Combined sources
Beta strandi32 – 376Combined sources
Helixi38 – 414Combined sources
Beta strandi49 – 513Combined sources
Helixi64 – 7310Combined sources
Beta strandi80 – 856Combined sources
Beta strandi87 – 915Combined sources
Helixi94 – 1007Combined sources
Beta strandi104 – 1096Combined sources
Helixi118 – 1236Combined sources
Beta strandi127 – 1348Combined sources
Helixi140 – 15112Combined sources
Beta strandi183 – 1853Combined sources
Helixi192 – 1954Combined sources
Helixi199 – 21719Combined sources
Helixi219 – 2235Combined sources
Helixi229 – 24214Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3KY7X-ray2.35A1-245[»]
ProteinModelPortaliQ6GHJ5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6GHJ5.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni131 – 1366S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the RNA methyltransferase TrmD family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000016242.
KOiK00554.
OMAiYKGVDQR.

Family and domain databases

Gene3Di1.10.1270.20. 1 hit.
3.40.1280.10. 1 hit.
HAMAPiMF_00605. TrmD. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR029026. tRNA_m1G_MTases_N.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view]
PfamiPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFiPIRSF000386. tRNA_mtase. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00088. trmD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6GHJ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIDYLTLFP EMFDGVLNHS IMKRAQENNK LQINTVNFRD YAINKHNQVD
60 70 80 90 100
DYPYGGGQGM VLKPEPVFNA MEDLDVTEQA RVILMCPQGE PFSHQKAVEL
110 120 130 140 150
SKADHIVFIC GHYEGYDERI RTHLVTDEIS MGDYVLTGGE LPAMTMTDAI
160 170 180 190 200
VRLIPGVLGN EQSHQDDSFS DGLLEFPQYT RPREFKGLTV PDVLLSGNHA
210 220 230 240
NIDAWRHEQK LIRTYNKRPD LIEKYPLTNA DKQILERYKI GLKKG
Length:245
Mass (Da):28,026
Last modified:July 19, 2004 - v1
Checksum:iF0C28BD78E7FF696
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571856 Genomic DNA. Translation: CAG40218.1.
RefSeqiWP_000687323.1. NC_002952.2.

Genome annotation databases

EnsemblBacteriaiCAG40218; CAG40218; SAR1216.
KEGGisar:SAR1216.
PATRICi19545979. VBIStaAur71814_1219.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571856 Genomic DNA. Translation: CAG40218.1.
RefSeqiWP_000687323.1. NC_002952.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3KY7X-ray2.35A1-245[»]
ProteinModelPortaliQ6GHJ5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAG40218; CAG40218; SAR1216.
KEGGisar:SAR1216.
PATRICi19545979. VBIStaAur71814_1219.

Phylogenomic databases

HOGENOMiHOG000016242.
KOiK00554.
OMAiYKGVDQR.

Enzyme and pathway databases

BioCyciSAUR282458:GJA5-1219-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ6GHJ5.

Family and domain databases

Gene3Di1.10.1270.20. 1 hit.
3.40.1280.10. 1 hit.
HAMAPiMF_00605. TrmD. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR029026. tRNA_m1G_MTases_N.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view]
PfamiPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFiPIRSF000386. tRNA_mtase. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00088. trmD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRMD_STAAR
AccessioniPrimary (citable) accession number: Q6GHJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: July 19, 2004
Last modified: September 7, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.