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Protein

tRNA (guanine-N(1)-)-methyltransferase

Gene

trmD

Organism
Staphylococcus aureus (strain MRSA252)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates guanosine-37 in various tRNAs.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei111S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(1)-)-methyltransferaseUniRule annotation (EC:2.1.1.228UniRule annotation)
Alternative name(s):
M1G-methyltransferaseUniRule annotation
tRNA [GM37] methyltransferaseUniRule annotation
Gene namesi
Name:trmDUniRule annotation
Ordered Locus Names:SAR1216
OrganismiStaphylococcus aureus (strain MRSA252)
Taxonomic identifieri282458 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000604571 – 245tRNA (guanine-N(1)-)-methyltransferaseAdd BLAST245

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

Secondary structure

1245
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 8Combined sources7
Helixi10 – 13Combined sources4
Helixi14 – 19Combined sources6
Turni20 – 22Combined sources3
Helixi23 – 27Combined sources5
Beta strandi32 – 37Combined sources6
Helixi38 – 41Combined sources4
Beta strandi49 – 51Combined sources3
Helixi64 – 73Combined sources10
Beta strandi80 – 85Combined sources6
Beta strandi87 – 91Combined sources5
Helixi94 – 100Combined sources7
Beta strandi104 – 109Combined sources6
Helixi118 – 123Combined sources6
Beta strandi127 – 134Combined sources8
Helixi140 – 151Combined sources12
Beta strandi183 – 185Combined sources3
Helixi192 – 195Combined sources4
Helixi199 – 217Combined sources19
Helixi219 – 223Combined sources5
Helixi229 – 242Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KY7X-ray2.35A1-245[»]
ProteinModelPortaliQ6GHJ5.
SMRiQ6GHJ5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6GHJ5.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni131 – 136S-adenosyl-L-methionine bindingUniRule annotation6

Sequence similaritiesi

Belongs to the RNA methyltransferase TrmD family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000016242.
KOiK00554.
OMAiYKGVDQR.

Family and domain databases

Gene3Di1.10.1270.20. 1 hit.
3.40.1280.10. 1 hit.
HAMAPiMF_00605. TrmD. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR029026. tRNA_m1G_MTases_N.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view]
PfamiPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFiPIRSF000386. tRNA_mtase. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00088. trmD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6GHJ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIDYLTLFP EMFDGVLNHS IMKRAQENNK LQINTVNFRD YAINKHNQVD
60 70 80 90 100
DYPYGGGQGM VLKPEPVFNA MEDLDVTEQA RVILMCPQGE PFSHQKAVEL
110 120 130 140 150
SKADHIVFIC GHYEGYDERI RTHLVTDEIS MGDYVLTGGE LPAMTMTDAI
160 170 180 190 200
VRLIPGVLGN EQSHQDDSFS DGLLEFPQYT RPREFKGLTV PDVLLSGNHA
210 220 230 240
NIDAWRHEQK LIRTYNKRPD LIEKYPLTNA DKQILERYKI GLKKG
Length:245
Mass (Da):28,026
Last modified:July 19, 2004 - v1
Checksum:iF0C28BD78E7FF696
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571856 Genomic DNA. Translation: CAG40218.1.
RefSeqiWP_000687323.1. NC_002952.2.

Genome annotation databases

EnsemblBacteriaiCAG40218; CAG40218; SAR1216.
KEGGisar:SAR1216.
PATRICi19545979. VBIStaAur71814_1219.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571856 Genomic DNA. Translation: CAG40218.1.
RefSeqiWP_000687323.1. NC_002952.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KY7X-ray2.35A1-245[»]
ProteinModelPortaliQ6GHJ5.
SMRiQ6GHJ5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAG40218; CAG40218; SAR1216.
KEGGisar:SAR1216.
PATRICi19545979. VBIStaAur71814_1219.

Phylogenomic databases

HOGENOMiHOG000016242.
KOiK00554.
OMAiYKGVDQR.

Miscellaneous databases

EvolutionaryTraceiQ6GHJ5.

Family and domain databases

Gene3Di1.10.1270.20. 1 hit.
3.40.1280.10. 1 hit.
HAMAPiMF_00605. TrmD. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR029026. tRNA_m1G_MTases_N.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view]
PfamiPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFiPIRSF000386. tRNA_mtase. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00088. trmD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRMD_STAAR
AccessioniPrimary (citable) accession number: Q6GHJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: July 19, 2004
Last modified: November 2, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.