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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Staphylococcus aureus (strain MRSA252)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi490MagnesiumUniRule annotation1
Metal bindingi496MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Synonyms:pnpA
Ordered Locus Names:SAR1250
OrganismiStaphylococcus aureus (strain MRSA252)
Taxonomic identifieri282458 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002600551 – 698Polyribonucleotide nucleotidyltransferaseAdd BLAST698

Interactioni

Protein-protein interaction databases

IntActiQ6GHG1. 1 interactor.

Structurei

3D structure databases

ProteinModelPortaliQ6GHG1.
SMRiQ6GHG1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini557 – 616KHUniRule annotationAdd BLAST60
Domaini626 – 694S1 motifUniRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218327.
KOiK00962.
OMAiRYMHNYN.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiView protein in InterPro
IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiView protein in Pfam
PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiView protein in SMART
SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiView protein in PROSITE
PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6GHG1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQEKKVFKT EWAGRSLTIE TGQLAKQANG AVLVRYGDTV VLSTATASKE
60 70 80 90 100
PRDGDFFPLT VNYEEKMYAA GKIPGGFKKR EGRPGDDATL TARLIDRPIR
110 120 130 140 150
PLFPKGYKHD VQIMNMVLSA DPDCSPQMAA MIGSSMALSV SDIPFQGPIA
160 170 180 190 200
GVNVGYIDGK YIINPTVEEK EVSRLDLEVA GHKDAVNMVE AGASEITEQE
210 220 230 240 250
MLEAIFFGHE EIQRLVDFQQ QIVDHIQPVK QEFIPTERDE ALVERVKSLT
260 270 280 290 300
EEKGLKETVL TFDKQQRDEN LDNLKEEIVN EFIDEEDPEN ELLIKEVYAI
310 320 330 340 350
LNELVKEEVR RLIADEKIRP DGRKPDEIRP LDSEVGILPR THGSGLFTRG
360 370 380 390 400
QTQALSVLTL GALGDYQLID GLGPEEEKRF MHHYNFPNFS VGETGPVRAP
410 420 430 440 450
GRREIGHGAL GERALKYIIP DTADFPYTIR IVSEVLESNG SSSQASICGS
460 470 480 490 500
TLALMDAGVP IKAPVAGIAM GLVTREDSYT ILTDIQGMED ALGDMDFKVA
510 520 530 540 550
GTKEGITAIQ MDIKIDGLTR EIIEEALEQA RRGRLEIMNH MLQTIDQPRT
560 570 580 590 600
ELSAYAPKVV TMTIKPDKIR DVIGPGGKKI NEIIDETGVK LDIEQDGTIF
610 620 630 640 650
IGAVDQAMIN RAREIIEEIT REAEVGQTYQ ATVKRIEKYG AFVGLFPGKD
660 670 680 690
ALLHISQISK NRIEKVEDVL KIGDTIEVKI TEIDKQGRVN ASHRALEE
Length:698
Mass (Da):77,392
Last modified:July 19, 2004 - v1
Checksum:i06C4666428CCB4F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571856 Genomic DNA. Translation: CAG40252.1.
RefSeqiWP_000076692.1. NC_002952.2.

Genome annotation databases

EnsemblBacteriaiCAG40252; CAG40252; SAR1250.
KEGGisar:SAR1250.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPNP_STAAR
AccessioniPrimary (citable) accession number: Q6GHG1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: July 19, 2004
Last modified: June 7, 2017
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families