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Protein

3-dehydroquinate synthase

Gene

aroB

Organism
Staphylococcus aureus (strain MRSA252)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate = 3-dehydroquinate + phosphate.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • NAD+UniRule annotation
  • Co2+UniRule annotation, Zn2+UniRule annotationNote: Binds 1 divalent metal cation per subunit. Can use either Co2+ or Zn2+.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroD)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

Cobalt, NAD, Zinc

Enzyme and pathway databases

BioCyciSAUR282458:GJA5-1479-MONOMER.
BRENDAi4.2.3.4. 3352.
UniPathwayiUPA00053; UER00085.

Names & Taxonomyi

Protein namesi
Recommended name:
3-dehydroquinate synthaseUniRule annotation (EC:4.2.3.4UniRule annotation)
Gene namesi
Name:aroBUniRule annotation
Ordered Locus Names:SAR1476
OrganismiStaphylococcus aureus (strain MRSA252)
Taxonomic identifieri282458 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3543543-dehydroquinate synthasePRO_0000140782Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

Secondary structure

1
354
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 43Combined sources
Beta strandi8 – 103Combined sources
Beta strandi13 – 175Combined sources
Helixi20 – 223Combined sources
Helixi23 – 286Combined sources
Beta strandi34 – 396Combined sources
Helixi40 – 5011Combined sources
Turni52 – 554Combined sources
Beta strandi59 – 646Combined sources
Helixi67 – 704Combined sources
Helixi73 – 8412Combined sources
Turni85 – 873Combined sources
Beta strandi93 – 997Combined sources
Helixi100 – 11213Combined sources
Helixi113 – 1153Combined sources
Beta strandi118 – 1236Combined sources
Helixi128 – 1314Combined sources
Beta strandi136 – 1394Combined sources
Beta strandi142 – 1443Combined sources
Beta strandi148 – 1514Combined sources
Beta strandi155 – 1606Combined sources
Helixi161 – 1666Combined sources
Helixi169 – 18618Combined sources
Helixi188 – 19710Combined sources
Helixi201 – 2066Combined sources
Turni207 – 2093Combined sources
Helixi210 – 22718Combined sources
Turni228 – 2303Combined sources
Beta strandi231 – 2333Combined sources
Helixi234 – 2396Combined sources
Turni240 – 2423Combined sources
Helixi243 – 25210Combined sources
Helixi256 – 27419Combined sources
Helixi281 – 29111Combined sources
Helixi297 – 3004Combined sources
Helixi306 – 3083Combined sources
Turni310 – 3123Combined sources
Beta strandi324 – 3285Combined sources
Beta strandi331 – 3344Combined sources
Helixi339 – 35113Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1XAGX-ray2.45A1-354[»]
1XAHX-ray2.20A/B1-354[»]
1XAIX-ray2.30A/B1-354[»]
1XAJX-ray2.35A/B1-354[»]
1XALX-ray2.80A/B1-354[»]
ProteinModelPortaliQ6GGU4.
SMRiQ6GGU4. Positions 1-353.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6GGU4.

Family & Domainsi

Sequence similaritiesi

Belongs to the dehydroquinate synthase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000007970.
KOiK01735.
OMAiHGAIKYI.
OrthoDBiEOG6SJJGD.

Family and domain databases

HAMAPiMF_00110. DHQ_synthase.
InterProiIPR016037. DHQ_synth_AroB.
IPR030963. DHQ_synth_fam.
IPR030960. DHQS/DOIS.
[Graphical view]
PfamiPF01761. DHQ_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001455. DHQ_synth. 1 hit.
TIGRFAMsiTIGR01357. aroB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6GGU4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLQTTYPSN NYPIFVEHGA IDHISTYIDQ FDQSFILIDE HVNQYFADKF
60 70 80 90 100
NDILSYENVH KVIIPAGEKT KTFEQYQETL EYILSHHVTR NTAIIAVGGG
110 120 130 140 150
ATGDFAGFVA ATLLRGVHFI QVPTTILAHD SSVGGKVGIN SKQGKNLIGA
160 170 180 190 200
FYRPTAVIYD LDFLKTLPFE QILSGYAEVY KHALLNGESA TQDIEQHFKD
210 220 230 240 250
REILQSLKGM DKYIAKGIET KLDIVVADEK EQGVRKFLNL GHTFGHAVEY
260 270 280 290 300
YHKIPHGHAV MVGIIYQFIV ANALFDSKHD INHYIQYLIQ LGYPLDMITD
310 320 330 340 350
LDFETLYQYM LSDKKNDKQG VQMVLIRQFG DIVVQHVDQL TLQHACEQLK

TYFK
Length:354
Mass (Da):40,285
Last modified:July 19, 2004 - v1
Checksum:i3B960112993F6F3F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571856 Genomic DNA. Translation: CAG40474.1.
RefSeqiWP_000776318.1. NC_002952.2.

Genome annotation databases

EnsemblBacteriaiCAG40474; CAG40474; SAR1476.
KEGGisar:SAR1476.
PATRICi19546502. VBIStaAur71814_1480.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571856 Genomic DNA. Translation: CAG40474.1.
RefSeqiWP_000776318.1. NC_002952.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1XAGX-ray2.45A1-354[»]
1XAHX-ray2.20A/B1-354[»]
1XAIX-ray2.30A/B1-354[»]
1XAJX-ray2.35A/B1-354[»]
1XALX-ray2.80A/B1-354[»]
ProteinModelPortaliQ6GGU4.
SMRiQ6GGU4. Positions 1-353.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAG40474; CAG40474; SAR1476.
KEGGisar:SAR1476.
PATRICi19546502. VBIStaAur71814_1480.

Phylogenomic databases

HOGENOMiHOG000007970.
KOiK01735.
OMAiHGAIKYI.
OrthoDBiEOG6SJJGD.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00085.
BioCyciSAUR282458:GJA5-1479-MONOMER.
BRENDAi4.2.3.4. 3352.

Miscellaneous databases

EvolutionaryTraceiQ6GGU4.

Family and domain databases

HAMAPiMF_00110. DHQ_synthase.
InterProiIPR016037. DHQ_synth_AroB.
IPR030963. DHQ_synth_fam.
IPR030960. DHQS/DOIS.
[Graphical view]
PfamiPF01761. DHQ_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001455. DHQ_synth. 1 hit.
TIGRFAMsiTIGR01357. aroB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MRSA252.

Entry informationi

Entry nameiAROB_STAAR
AccessioniPrimary (citable) accession number: Q6GGU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: July 19, 2004
Last modified: March 16, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.