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Protein

3-dehydroquinate synthase

Gene

aroB

Organism
Staphylococcus aureus (strain MRSA252)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate = 3-dehydroquinate + phosphate.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • NAD+UniRule annotation
  • Co2+UniRule annotation, Zn2+UniRule annotationNote: Binds 1 divalent metal cation per subunit. Can use either Co2+ or Zn2+.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroD)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

Cobalt, NAD, Zinc

Enzyme and pathway databases

BRENDAi4.2.3.4. 3352.
UniPathwayiUPA00053; UER00085.

Names & Taxonomyi

Protein namesi
Recommended name:
3-dehydroquinate synthaseUniRule annotation (EC:4.2.3.4UniRule annotation)
Gene namesi
Name:aroBUniRule annotation
Ordered Locus Names:SAR1476
OrganismiStaphylococcus aureus (strain MRSA252)
Taxonomic identifieri282458 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001407821 – 3543-dehydroquinate synthaseAdd BLAST354

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

Secondary structure

1354
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 4Combined sources3
Beta strandi8 – 10Combined sources3
Beta strandi13 – 17Combined sources5
Helixi20 – 22Combined sources3
Helixi23 – 28Combined sources6
Beta strandi34 – 39Combined sources6
Helixi40 – 50Combined sources11
Turni52 – 55Combined sources4
Beta strandi59 – 64Combined sources6
Helixi67 – 70Combined sources4
Helixi73 – 84Combined sources12
Turni85 – 87Combined sources3
Beta strandi93 – 99Combined sources7
Helixi100 – 112Combined sources13
Helixi113 – 115Combined sources3
Beta strandi118 – 123Combined sources6
Helixi128 – 131Combined sources4
Beta strandi136 – 139Combined sources4
Beta strandi142 – 144Combined sources3
Beta strandi148 – 151Combined sources4
Beta strandi155 – 160Combined sources6
Helixi161 – 166Combined sources6
Helixi169 – 186Combined sources18
Helixi188 – 197Combined sources10
Helixi201 – 206Combined sources6
Turni207 – 209Combined sources3
Helixi210 – 227Combined sources18
Turni228 – 230Combined sources3
Beta strandi231 – 233Combined sources3
Helixi234 – 239Combined sources6
Turni240 – 242Combined sources3
Helixi243 – 252Combined sources10
Helixi256 – 274Combined sources19
Helixi281 – 291Combined sources11
Helixi297 – 300Combined sources4
Helixi306 – 308Combined sources3
Turni310 – 312Combined sources3
Beta strandi324 – 328Combined sources5
Beta strandi331 – 334Combined sources4
Helixi339 – 351Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XAGX-ray2.45A1-354[»]
1XAHX-ray2.20A/B1-354[»]
1XAIX-ray2.30A/B1-354[»]
1XAJX-ray2.35A/B1-354[»]
1XALX-ray2.80A/B1-354[»]
ProteinModelPortaliQ6GGU4.
SMRiQ6GGU4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6GGU4.

Family & Domainsi

Sequence similaritiesi

Belongs to the dehydroquinate synthase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000007970.
KOiK01735.
OMAiTMSETIY.

Family and domain databases

HAMAPiMF_00110. DHQ_synthase. 1 hit.
InterProiIPR016037. DHQ_synth_AroB.
IPR030963. DHQ_synth_fam.
IPR030960. DHQS/DOIS.
[Graphical view]
PfamiPF01761. DHQ_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001455. DHQ_synth. 1 hit.
TIGRFAMsiTIGR01357. aroB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6GGU4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLQTTYPSN NYPIFVEHGA IDHISTYIDQ FDQSFILIDE HVNQYFADKF
60 70 80 90 100
NDILSYENVH KVIIPAGEKT KTFEQYQETL EYILSHHVTR NTAIIAVGGG
110 120 130 140 150
ATGDFAGFVA ATLLRGVHFI QVPTTILAHD SSVGGKVGIN SKQGKNLIGA
160 170 180 190 200
FYRPTAVIYD LDFLKTLPFE QILSGYAEVY KHALLNGESA TQDIEQHFKD
210 220 230 240 250
REILQSLKGM DKYIAKGIET KLDIVVADEK EQGVRKFLNL GHTFGHAVEY
260 270 280 290 300
YHKIPHGHAV MVGIIYQFIV ANALFDSKHD INHYIQYLIQ LGYPLDMITD
310 320 330 340 350
LDFETLYQYM LSDKKNDKQG VQMVLIRQFG DIVVQHVDQL TLQHACEQLK

TYFK
Length:354
Mass (Da):40,285
Last modified:July 19, 2004 - v1
Checksum:i3B960112993F6F3F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571856 Genomic DNA. Translation: CAG40474.1.
RefSeqiWP_000776318.1. NC_002952.2.

Genome annotation databases

EnsemblBacteriaiCAG40474; CAG40474; SAR1476.
KEGGisar:SAR1476.
PATRICi19546502. VBIStaAur71814_1480.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571856 Genomic DNA. Translation: CAG40474.1.
RefSeqiWP_000776318.1. NC_002952.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XAGX-ray2.45A1-354[»]
1XAHX-ray2.20A/B1-354[»]
1XAIX-ray2.30A/B1-354[»]
1XAJX-ray2.35A/B1-354[»]
1XALX-ray2.80A/B1-354[»]
ProteinModelPortaliQ6GGU4.
SMRiQ6GGU4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAG40474; CAG40474; SAR1476.
KEGGisar:SAR1476.
PATRICi19546502. VBIStaAur71814_1480.

Phylogenomic databases

HOGENOMiHOG000007970.
KOiK01735.
OMAiTMSETIY.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00085.
BRENDAi4.2.3.4. 3352.

Miscellaneous databases

EvolutionaryTraceiQ6GGU4.

Family and domain databases

HAMAPiMF_00110. DHQ_synthase. 1 hit.
InterProiIPR016037. DHQ_synth_AroB.
IPR030963. DHQ_synth_fam.
IPR030960. DHQS/DOIS.
[Graphical view]
PfamiPF01761. DHQ_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001455. DHQ_synth. 1 hit.
TIGRFAMsiTIGR01357. aroB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAROB_STAAR
AccessioniPrimary (citable) accession number: Q6GGU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: July 19, 2004
Last modified: November 2, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.