Q6GFZ8 (DHA2_STAAR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine dehydrogenase 2
EC=1.4.1.1

Gene names

Name:	ald2
Ordered Locus Names:	SAR1787

Organism

Staphylococcus aureus (strain MRSA252) [Complete proteome] [HAMAP]

Taxonomic identifier

282458 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May play a role in cell wall synthesis as L-alanine is an important constituent of the peptidoglycan layer By similarity.
Catalytic activity	L-alanine + H2O + NAD+ = pyruvate + NH3 + NADH.
Pathway	Amino-acid degradation; L-alanine degradation via dehydrogenase pathway; NH(3) and pyruvate from L-alanine: step 1/1.
Sequence similarities	Belongs to the AlaDH/PNT family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	alanine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Alanine dehydrogenase 2		PRO_0000199004
Regions
	Nucleotide binding	169 – 199	31	NAD By similarity
Sites
	Active site	95	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6GFZ8 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 32AD0DA4734EC9FB	FASTA	372	40,114
	10 20 30 40 50 60 MKIGIPREIK NNENRVGLSP SGVHALVESG HTVLVETNAG SGSFFEDVDY KEAGAEIVAE 70 80 90 100 110 120 QAKVWDVDMV IKVKEPLESE YPYFKEGLVL FTYLHLANEE KLTQALIDRK VISIAYETVQ 130 140 150 160 170 180 LPDRSLPLLS PMSEVAGRMS AQVGAEFLQK LNGGMGILLG GVPGVPKGKV TIIGGGQAGT 190 200 210 220 230 240 NAAKIALGLG ADVTILDVNP KRLQQLDDLF GGRVHTIMSN PLNIELYVKQ SDLVIGAVLI 250 260 270 280 290 300 PGAKAPRLVT EDMIKQMKNG SVIIDIAIDQ GGIFETTDKI TTHDDPTYIK HGVVHYAVAN 310 320 330 340 350 360 MPGAVPRTST LALNNATLPY ALMLANKGYR EAFKSNQPLS LGLNTYKGHV TNKGVAEAFE 370 MEYKSVEEAL HL

References

[1]

"Complete genomes of two clinical Staphylococcus aureus strains: evidence for the rapid evolution of virulence and drug resistance." Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J., Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A., Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C., Clark L., Corton C. , Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z., Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N., Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M., Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004) [PubMed: 15213324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MRSA252.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571856 Genomic DNA. Translation: CAG40778.1.
RefSeq	YP_041174.1. NC_002952.2.
3D structure databases
ProteinModelPortal	Q6GFZ8.
SMR	Q6GFZ8. Positions 1-371.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6GFZ8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000019664; EBSTAP00000019000; EBSTAG00000019663.
GeneID	2860988.
GenomeReviews	Gene locus SAR1787 in contig BX571856_GR.
KEGG	sar:SAR1787.
PATRIC	19547124. VBIStaAur71814_1791.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0686.
GeneTree	EBGT00050000024386.
HOGENOM	HBG289735.
OMA	VAHGHEV.
PhylomeDB	Q6GFZ8.
ProtClustDB	CLSK885510.
Enzyme and pathway databases
BioCyc	SAUR282458:SAR1787-MONOMER.
Family and domain databases
InterPro	IPR008142. Ala_DH/PNT_CS1. IPR008143. Ala_DH/PNT_CS2. IPR008141. Ala_DH/Pyr_transHydgase. IPR007886. AlaDH/PNT_N. IPR007698. AlaDH/PNT_NAD(H)-bd. [Graphical view]
KO	K00259.
PANTHER	PTHR10160:SF3. PTHR10160:SF3. 1 hit.
Pfam	PF01262. AlaDh_PNT_C. 1 hit. PF05222. AlaDh_PNT_N. 1 hit. [Graphical view]
PIRSF	PIRSF000183. Alanine_dh. 1 hit.
SMART	SM01002. AlaDh_PNT_C. 1 hit. SM01003. AlaDh_PNT_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00518. AlaDH. 1 hit.
PROSITE	PS00836. ALADH_PNT_1. 1 hit. PS00837. ALADH_PNT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHA2_STAAR

Accession

Primary (citable) accession number: Q6GFZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	July 19, 2004
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program