Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA polymerase IV

Gene

dinB

Organism
Staphylococcus aureus (strain MRSA252)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII.UniRule annotation

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi10MagnesiumUniRule annotation1
Sitei15Substrate discriminationUniRule annotation1
Metal bindingi105MagnesiumUniRule annotation1
Active sitei106UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase
Biological processDNA damage, DNA repair, DNA replication
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase IVUniRule annotation (EC:2.7.7.7UniRule annotation)
Short name:
Pol IVUniRule annotation
Gene namesi
Name:dinBUniRule annotation
Ordered Locus Names:SAR1986
OrganismiStaphylococcus aureus (strain MRSA252)
Taxonomic identifieri282458 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001739461 – 356DNA polymerase IVAdd BLAST356

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ6GFG2
SMRiQ6GFG2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 187UmuCUniRule annotationAdd BLAST182

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000082707
KOiK02346
OMAiGMQIREI

Family and domain databases

CDDicd03586 PolY_Pol_IV_kappa, 1 hit
Gene3Di3.30.1490.100, 1 hit
HAMAPiMF_01113 DNApol_IV, 1 hit
InterProiView protein in InterPro
IPR036775 DNA_pol_Y-fam_lit_finger_sf
IPR017961 DNA_pol_Y-fam_little_finger
IPR022880 DNApol_IV
IPR001126 UmuC
PfamiView protein in Pfam
PF00817 IMS, 1 hit
PF11799 IMS_C, 1 hit
SUPFAMiSSF100879 SSF100879, 1 hit
PROSITEiView protein in PROSITE
PS50173 UMUC, 1 hit

Sequencei

Sequence statusi: Complete.

Q6GFG2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTERRIIHID MDYFFAQVEM RDNPKLKGKP VIVGGKASSR GVVSTASYEA
60 70 80 90 100
RKYGVHSAMP MSQAHKLCPN GYFVTSNFGA YRETSAQIMS IFRSYTDKVE
110 120 130 140 150
PMSLDEAYLD ITELVRPDLP ASKIAQYIRK DILEQTHLTA SAGVSYNKFL
160 170 180 190 200
AKLASGMNKP DGMTVIDYRN VHDILMTLDI GDFPGVGKAS KKVMHDNGIF
210 220 230 240 250
NGRDLYEKTE FELIRLFGKR GRGLYNKARG IDHSEVKSTR VRKSVGTERT
260 270 280 290 300
FATDVNDDEE ILRKVWELSG KTAERLNKLQ KSAKTVTVKI KTYQFETLSK
310 320 330 340 350
QMSLRDSVSS EEDIYNIAYL LYNDLKDPDV PIRLIGVTVG NLEQSTYKNM

TIYDFI
Length:356
Mass (Da):40,357
Last modified:July 19, 2004 - v1
Checksum:i0370CC25A3C42143
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571856 Genomic DNA Translation: CAG40972.1
RefSeqiWP_000140179.1, NC_002952.2

Genome annotation databases

EnsemblBacteriaiCAG40972; CAG40972; SAR1986
KEGGisar:SAR1986

Similar proteinsi

Entry informationi

Entry nameiDPO4_STAAR
AccessioniPrimary (citable) accession number: Q6GFG2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: July 19, 2004
Last modified: March 28, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health