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Q6GFF9

- DAGK_STAAR

UniProt

Q6GFF9 - DAGK_STAAR

Protein

Diacylglycerol kinase

Gene

dagK

Organism
Staphylococcus aureus (strain MRSA252)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (19 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthetic pathway.1 Publication

    Catalytic activityi

    ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

    Cofactori

    Binds 1 magnesium ion per subunit. This ion appears to have a structural role and is required for catalytic activity.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei41 – 411ATP
    Binding sitei94 – 941ATP
    Metal bindingi213 – 2131Magnesium; via carbonyl oxygen1 Publication
    Metal bindingi216 – 2161Magnesium1 Publication
    Metal bindingi218 – 2181Magnesium; via carbonyl oxygen1 Publication
    Active sitei273 – 2731Proton acceptor1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 145ATP
    Nucleotide bindingi67 – 737ATP

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. diacylglycerol kinase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW
    4. NAD+ kinase activity Source: InterPro

    GO - Biological processi

    1. phospholipid biosynthetic process Source: UniProtKB-KW
    2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSAUR282458:GJA5-2022-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol kinase (EC:2.7.1.107)
    Short name:
    DAG kinase
    Short name:
    DAGK
    Gene namesi
    Name:dagK
    Synonyms:dgkB
    Ordered Locus Names:SAR1989
    OrganismiStaphylococcus aureus (strain MRSA252)
    Taxonomic identifieri282458 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000000596: Chromosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi68 – 681D → A: Loss of activity. 1 Publication
    Mutagenesisi91 – 911P → A: Loss of activity. 1 Publication
    Mutagenesisi94 – 941T → A: Loss of activity. 1 Publication
    Mutagenesisi96 – 961N → A: Loss of activity. 1 Publication
    Mutagenesisi97 – 971D → A: Loss of activity. 1 Publication
    Mutagenesisi124 – 1241D → A: Loss of activity. 1 Publication
    Mutagenesisi168 – 1681E → A: No effect on activity. 1 Publication
    Mutagenesisi216 – 2161D → A: 5-fold decrease in activity. 1 Publication
    Mutagenesisi271 – 2711D → A: Loss of activity. 1 Publication
    Mutagenesisi273 – 2731E → A: Loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 315315Diacylglycerol kinasePRO_0000386495Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    DIPiDIP-46022N.
    STRINGi282458.SAR1989.

    Structurei

    Secondary structure

    1
    315
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 97
    Helixi18 – 3114
    Beta strandi34 – 407
    Helixi46 – 549
    Turni55 – 584
    Beta strandi60 – 667
    Helixi68 – 7811
    Beta strandi86 – 916
    Helixi97 – 1015
    Helixi108 – 11710
    Beta strandi120 – 1289
    Beta strandi131 – 14010
    Helixi159 – 1613
    Turni166 – 1694
    Helixi170 – 1723
    Beta strandi176 – 1827
    Beta strandi185 – 19915
    Beta strandi214 – 2174
    Beta strandi219 – 2257
    Helixi229 – 23911
    Turni240 – 2423
    Helixi244 – 2463
    Beta strandi250 – 26112
    Beta strandi267 – 2704
    Beta strandi273 – 2786
    Beta strandi280 – 29213
    Beta strandi295 – 2973

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2QV7X-ray2.30A1-315[»]
    2QVLX-ray2.40A1-315[»]
    ProteinModelPortaliQ6GFF9.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ6GFF9.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 132132DAGKcPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the diacylglycerol/lipid kinase family.Curated
    Contains 1 DAGKc domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1597.
    HOGENOMiHOG000261395.
    KOiK07029.
    OMAiYLKGMEM.
    OrthoDBiEOG613098.

    Family and domain databases

    Gene3Di3.40.50.10330. 1 hit.
    InterProiIPR017438. ATP-NAD_kinase_dom_1.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR005218. Diacylglycerol/lipid_kinase.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    [Graphical view]
    PfamiPF00781. DAGK_cat. 1 hit.
    [Graphical view]
    SMARTiSM00046. DAGKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.
    TIGRFAMsiTIGR00147. TIGR00147. 1 hit.
    PROSITEiPS50146. DAGK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6GFF9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRKRARIIYN PTSGKEQFKR ELPDALIKLE KAGYETSAYA TEKIGDATLE    50
    AERAMHENYD VLIAAGGDGT LNEVVNGIAE KPNRPKLGVI PMGTVNDFGR 100
    ALHIPNDIMG ALDVIIEGHS TKVDIGKMNN RYFINLAAGG QLTQVSYETP 150
    SKLKSIVGPF AYYIKGFEML PQMKAVDLRI EYDGNVFQGE ALLFFLGLTN 200
    SMAGFEKLVP DAKLDDGYFT LIIVEKSNLA ELGHIMTLAS RGEHTKHPKV 250
    IYEKAKAINI SSFTDLQLNV DGEYGGKLPA NFLNLERHID VFAPNDIVNE 300
    ELINNDHVDD NLIEE 315
    Length:315
    Mass (Da):34,902
    Last modified:July 19, 2004 - v1
    Checksum:i5EA85BFC769E1D0C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX571856 Genomic DNA. Translation: CAG40975.1.
    RefSeqiYP_041363.1. NC_002952.2.

    Genome annotation databases

    EnsemblBacteriaiCAG40975; CAG40975; SAR1989.
    GeneIDi2859730.
    KEGGisar:SAR1989.
    PATRICi19547619. VBIStaAur71814_2000.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX571856 Genomic DNA. Translation: CAG40975.1 .
    RefSeqi YP_041363.1. NC_002952.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2QV7 X-ray 2.30 A 1-315 [» ]
    2QVL X-ray 2.40 A 1-315 [» ]
    ProteinModelPortali Q6GFF9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-46022N.
    STRINGi 282458.SAR1989.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAG40975 ; CAG40975 ; SAR1989 .
    GeneIDi 2859730.
    KEGGi sar:SAR1989.
    PATRICi 19547619. VBIStaAur71814_2000.

    Phylogenomic databases

    eggNOGi COG1597.
    HOGENOMi HOG000261395.
    KOi K07029.
    OMAi YLKGMEM.
    OrthoDBi EOG613098.

    Enzyme and pathway databases

    BioCyci SAUR282458:GJA5-2022-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q6GFF9.

    Family and domain databases

    Gene3Di 3.40.50.10330. 1 hit.
    InterProi IPR017438. ATP-NAD_kinase_dom_1.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR005218. Diacylglycerol/lipid_kinase.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    [Graphical view ]
    Pfami PF00781. DAGK_cat. 1 hit.
    [Graphical view ]
    SMARTi SM00046. DAGKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 1 hit.
    TIGRFAMsi TIGR00147. TIGR00147. 1 hit.
    PROSITEi PS50146. DAGK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MRSA252.
    2. "Identification of a soluble diacylglycerol kinase required for lipoteichoic acid production in Bacillus subtilis."
      Jerga A., Lu Y.-J., Schujman G.E., de Mendoza D., Rock C.O.
      J. Biol. Chem. 282:21738-21745(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A DIACYLGLYCEROL KINASE.
    3. "Analysis of the Staphylococcus aureus DgkB structure reveals a common catalytic mechanism for the soluble diacylglycerol kinases."
      Miller D.J., Jerga A., Rock C.O., White S.W.
      Structure 16:1036-1046(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF APOENZYME AND IN COMPLEX WITH ADP AND MAGNESIUM, SUBUNIT, COFACTOR, MUTAGENESIS OF ASP-68; PRO-91; THR-94; ASN-96; ASP-97; ASP-124; GLU-168; ASP-216; ASP-271 AND GLU-273, ACTIVE SITE, REACTION MECHANISM.

    Entry informationi

    Entry nameiDAGK_STAAR
    AccessioniPrimary (citable) accession number: Q6GFF9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 13, 2009
    Last sequence update: July 19, 2004
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3