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Protein

Diacylglycerol kinase

Gene

dagK

Organism
Staphylococcus aureus (strain MRSA252)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthetic pathway.1 Publication

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Cofactori

Mg2+1 PublicationNote: Binds 1 Mg2+ ion per subunit. This ion appears to have a structural role and is required for catalytic activity.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411ATP
Binding sitei94 – 941ATP
Metal bindingi213 – 2131Magnesium; via carbonyl oxygen1 Publication
Metal bindingi216 – 2161Magnesium1 Publication
Metal bindingi218 – 2181Magnesium; via carbonyl oxygen1 Publication
Active sitei273 – 2731Proton acceptor1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 145ATP
Nucleotide bindingi67 – 737ATP

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. diacylglycerol kinase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. NAD+ kinase activity Source: InterPro

GO - Biological processi

  1. phospholipid biosynthetic process Source: UniProtKB-KW
  2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAUR282458:GJA5-2022-MONOMER.
BRENDAi2.7.1.107. 3352.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase (EC:2.7.1.107)
Short name:
DAG kinase
Short name:
DAGK
Gene namesi
Name:dagK
Synonyms:dgkB
Ordered Locus Names:SAR1989
OrganismiStaphylococcus aureus (strain MRSA252)
Taxonomic identifieri282458 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000000596 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi68 – 681D → A: Loss of activity. 1 Publication
Mutagenesisi91 – 911P → A: Loss of activity. 1 Publication
Mutagenesisi94 – 941T → A: Loss of activity. 1 Publication
Mutagenesisi96 – 961N → A: Loss of activity. 1 Publication
Mutagenesisi97 – 971D → A: Loss of activity. 1 Publication
Mutagenesisi124 – 1241D → A: Loss of activity. 1 Publication
Mutagenesisi168 – 1681E → A: No effect on activity. 1 Publication
Mutagenesisi216 – 2161D → A: 5-fold decrease in activity. 1 Publication
Mutagenesisi271 – 2711D → A: Loss of activity. 1 Publication
Mutagenesisi273 – 2731E → A: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315Diacylglycerol kinasePRO_0000386495Add
BLAST

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

DIPiDIP-46022N.
STRINGi282458.SAR1989.

Structurei

Secondary structure

1
315
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 97Combined sources
Helixi18 – 3114Combined sources
Beta strandi34 – 407Combined sources
Helixi46 – 549Combined sources
Turni55 – 584Combined sources
Beta strandi60 – 667Combined sources
Helixi68 – 7811Combined sources
Beta strandi86 – 916Combined sources
Helixi97 – 1015Combined sources
Helixi108 – 11710Combined sources
Beta strandi120 – 1289Combined sources
Beta strandi131 – 14010Combined sources
Helixi159 – 1613Combined sources
Turni166 – 1694Combined sources
Helixi170 – 1723Combined sources
Beta strandi176 – 1827Combined sources
Beta strandi185 – 19915Combined sources
Beta strandi214 – 2174Combined sources
Beta strandi219 – 2257Combined sources
Helixi229 – 23911Combined sources
Turni240 – 2423Combined sources
Helixi244 – 2463Combined sources
Beta strandi250 – 26112Combined sources
Beta strandi267 – 2704Combined sources
Beta strandi273 – 2786Combined sources
Beta strandi280 – 29213Combined sources
Beta strandi295 – 2973Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2QV7X-ray2.30A1-315[»]
2QVLX-ray2.40A1-315[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6GFF9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 132132DAGKcPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the diacylglycerol/lipid kinase family.Curated
Contains 1 DAGKc domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1597.
HOGENOMiHOG000261395.
KOiK07029.
OMAiRGEHIND.
OrthoDBiEOG613098.

Family and domain databases

Gene3Di3.40.50.10330. 1 hit.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR005218. Diacylglycerol/lipid_kinase.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR016064. NAD/diacylglycerol_kinase.
[Graphical view]
PfamiPF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
TIGRFAMsiTIGR00147. TIGR00147. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6GFF9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKRARIIYN PTSGKEQFKR ELPDALIKLE KAGYETSAYA TEKIGDATLE
60 70 80 90 100
AERAMHENYD VLIAAGGDGT LNEVVNGIAE KPNRPKLGVI PMGTVNDFGR
110 120 130 140 150
ALHIPNDIMG ALDVIIEGHS TKVDIGKMNN RYFINLAAGG QLTQVSYETP
160 170 180 190 200
SKLKSIVGPF AYYIKGFEML PQMKAVDLRI EYDGNVFQGE ALLFFLGLTN
210 220 230 240 250
SMAGFEKLVP DAKLDDGYFT LIIVEKSNLA ELGHIMTLAS RGEHTKHPKV
260 270 280 290 300
IYEKAKAINI SSFTDLQLNV DGEYGGKLPA NFLNLERHID VFAPNDIVNE
310
ELINNDHVDD NLIEE
Length:315
Mass (Da):34,902
Last modified:July 19, 2004 - v1
Checksum:i5EA85BFC769E1D0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571856 Genomic DNA. Translation: CAG40975.1.
RefSeqiYP_041363.1. NC_002952.2.

Genome annotation databases

EnsemblBacteriaiCAG40975; CAG40975; SAR1989.
KEGGisar:SAR1989.
PATRICi19547619. VBIStaAur71814_2000.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571856 Genomic DNA. Translation: CAG40975.1.
RefSeqiYP_041363.1. NC_002952.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2QV7X-ray2.30A1-315[»]
2QVLX-ray2.40A1-315[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46022N.
STRINGi282458.SAR1989.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAG40975; CAG40975; SAR1989.
KEGGisar:SAR1989.
PATRICi19547619. VBIStaAur71814_2000.

Phylogenomic databases

eggNOGiCOG1597.
HOGENOMiHOG000261395.
KOiK07029.
OMAiRGEHIND.
OrthoDBiEOG613098.

Enzyme and pathway databases

BioCyciSAUR282458:GJA5-2022-MONOMER.
BRENDAi2.7.1.107. 3352.

Miscellaneous databases

EvolutionaryTraceiQ6GFF9.

Family and domain databases

Gene3Di3.40.50.10330. 1 hit.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR005218. Diacylglycerol/lipid_kinase.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR016064. NAD/diacylglycerol_kinase.
[Graphical view]
PfamiPF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
TIGRFAMsiTIGR00147. TIGR00147. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MRSA252.
  2. "Identification of a soluble diacylglycerol kinase required for lipoteichoic acid production in Bacillus subtilis."
    Jerga A., Lu Y.-J., Schujman G.E., de Mendoza D., Rock C.O.
    J. Biol. Chem. 282:21738-21745(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A DIACYLGLYCEROL KINASE.
  3. "Analysis of the Staphylococcus aureus DgkB structure reveals a common catalytic mechanism for the soluble diacylglycerol kinases."
    Miller D.J., Jerga A., Rock C.O., White S.W.
    Structure 16:1036-1046(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF APOENZYME AND IN COMPLEX WITH ADP AND MAGNESIUM, SUBUNIT, COFACTOR, MUTAGENESIS OF ASP-68; PRO-91; THR-94; ASN-96; ASP-97; ASP-124; GLU-168; ASP-216; ASP-271 AND GLU-273, ACTIVE SITE, REACTION MECHANISM.

Entry informationi

Entry nameiDAGK_STAAR
AccessioniPrimary (citable) accession number: Q6GFF9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 13, 2009
Last sequence update: July 19, 2004
Last modified: April 29, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.