Reviewed, UniProtKB/Swiss-Prot Q6GFE8 (SSPP_STAAR)

Names and origin

Protein names

Recommended name:
    Staphopain A
    EC=3.4.22.48
Alternative name(s):
    Staphylopain A
    Staphylococcal cysteine proteinase A

Gene names

Name:	sspP
Synonyms:	scpA
Ordered Locus Names:	SAR2001

Organism

Staphylococcus aureus (strain MRSA252) [Complete proteome] [HAMAP]

Taxonomic identifier

282458 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cysteine protease able to degrade elastin By similarity.
Catalytic activity	Broad endopeptidase action on proteins including elastin, but rather limited hydrolysis of small-molecule substrates. Assays are conveniently made with hemoglobin, casein or Z-Phe-Arg-NHMec as substrate.
Enzyme regulation	Prematurely activated/folded staphopain A is inhibited by staphostatin A (scpB), which is probably required to protect staphylococcal cytoplasmic proteins from degradation by scpA By similarity.
Subunit structure	In the cytoplasm, prematurely activated/folded scpA forms a stable non-covalent complex with scpB By similarity.
Subcellular location	Secreted By similarity.
Post-translational modification	Cleavage leads to the activation of scpA probably by an auto-catalytic manner By similarity.
Miscellaneous	The catalytic maturation of scpA appears to reside outside the cascade of activation started by the metalloprotease aureolysin (aur) By similarity.
Sequence similarities	Belongs to the peptidase C47 family.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Propeptide	26 – 214	189	By similarity		PRO_0000026553
	Chain	215 – 388	174	Staphopain A		PRO_0000026554
Sites
	Active site	238	1	By similarity
	Active site	334	1	By similarity
	Active site	355	1	By similarity
	Site	214 – 215	2	Cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6GFE8-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 44017EA70E289F14	FASTA	388	44,048
	10 20 30 40 50 60 MKRNFPKLIA LSLIFSLSIT PIANAESNSN IKAKDKRHVQ VNVEDKSVPT DVRNLAQKDY 70 80 90 100 110 120 LSYVTSLDKI YNKEKASYTL GEPFKIYKFN KKSDGNYYFP VLNTEGNIDY IVTISPKVTK 130 140 150 160 170 180 DSSSSSKYTI NVSSFLSKAL NEYKDQQITI LTNSKGYYVV TQNHKAKLVL KTPRLEDKKA 190 200 210 220 230 240 KKTESIPTGN NVTQLKQKAS VTMPTSQFKS NNYTYNEQYV NKLENFKIRE TQGNNGWCAG 250 260 270 280 290 300 YTMSALLNAT YNTNKYHAEA VMRFLHPNLQ GQQFQFTGLT PREMIYFGQT QGRSPQLLNR 310 320 330 340 350 360 MTTYNEVDNL TKNNKGIAIL GSRVESRNGM HAGHAMAVVG NAKLNNGQEV IIIWNPWDNG 370 380 FMTQDAKNNV IPVSNGDHYQ WYSSIYGY

References

[1]

"Complete genomes of two clinical Staphylococcus aureus strains: evidence for the rapid evolution of virulence and drug resistance." Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J., Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A., Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C., Clark L., Corton C. , Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z., Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N., Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M., Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004) [PubMed: 15213324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX571856 Genomic DNA. Translation: CAG40986.1.
RefSeq	YP_041374.1.
3D structure databases
SMR	Q6GFE8. Positions 216-388.
ModBase	Search...
Genome annotation databases
GeneID	2859733.
GenomeReviews	Gene locus SAR2001 in contig BX571856_GR.
KEGG	sar:SAR2001.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6GFE8.
OMA	Q6GFE8. YTINVSS.
Enzyme and pathway databases
BioCyc	SAUR282458:SAR2001-MON.
Family and domain databases
InterPro	IPR000169. Pept_cys_AS. IPR008750. Peptidase_C47. [Graphical view]
Pfam	PF05543. Peptidase_C47. 1 hit. [Graphical view]
PROSITE	PS00640. THIOL_PROTEASE_ASN. False negative. PS00139. THIOL_PROTEASE_CYS. False negative. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SSPP_STAAR

Accession

Primary (citable) accession number: Q6GFE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	July 19, 2004
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)