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Protein

NH(3)-dependent NAD(+) synthetase

Gene

nadE

Organism
Staphylococcus aureus (strain MRSA252)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.UniRule annotation

Catalytic activityi

ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. NH(3)-dependent NAD(+) synthetase (nadE)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi53MagnesiumUniRule annotation1
Binding sitei139Deamido-NADUniRule annotation1
Binding sitei159ATPUniRule annotation1
Metal bindingi164MagnesiumUniRule annotation1
Binding sitei172Deamido-NADUniRule annotation1
Binding sitei179Deamido-NADUniRule annotation1
Binding sitei188ATPUniRule annotation1
Binding sitei210ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi47 – 54ATPUniRule annotation8
Nucleotide bindingi259 – 260Deamido-NADUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00253; UER00333.

Names & Taxonomyi

Protein namesi
Recommended name:
NH(3)-dependent NAD(+) synthetaseUniRule annotation (EC:6.3.1.5UniRule annotation)
Gene namesi
Name:nadEUniRule annotation
Ordered Locus Names:SAR2005
OrganismiStaphylococcus aureus (strain MRSA252)
Taxonomic identifieri282458 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001521971 – 273NH(3)-dependent NAD(+) synthetaseAdd BLAST273

Proteomic databases

PRIDEiQ6GFE4.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ6GFE4.
SMRiQ6GFE4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD synthetase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000238070.
KOiK01916.
OMAiCAINPIG.

Family and domain databases

CDDicd00553. NAD_synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE_ammonia_dep. 1 hit.
InterProiView protein in InterPro
IPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
PANTHERiPTHR23090. PTHR23090. 1 hit.
PfamiView protein in Pfam
PF02540. NAD_synthase. 1 hit.
TIGRFAMsiTIGR00552. nadE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6GFE4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKLQDVIVQ EMKVKKRIDS AEEIAELKQF IKSYVQSHSF IKSLVLGISG
60 70 80 90 100
GQDSTLVGKL VQMSVNELRE EGIDCTFIAV KLPYGVQKDA DEVEQALQFI
110 120 130 140 150
EPDEIVTVNI KPAVDQSVQS LKEAGIVLTD FQKGNEKARE RMKVQFSIAS
160 170 180 190 200
NRQGIVVGTD HSAENITGFY TKYGDGAADI APIFGLNKRQ GRQLLAYLGA
210 220 230 240 250
PKQLYEKTPT ADLEDDKPQL PDEDALGVTY EAIDNYLEGK PVTPEEQKVI
260 270
ENHYIRNAHK RELAYTRYTW PKS
Length:273
Mass (Da):30,581
Last modified:July 19, 2004 - v1
Checksum:iA369E3B1D6F6EFA5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571856 Genomic DNA. Translation: CAG40990.1.
RefSeqiWP_000040861.1. NC_002952.2.

Genome annotation databases

EnsemblBacteriaiCAG40990; CAG40990; SAR2005.
KEGGisar:SAR2005.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiNADE_STAAR
AccessioniPrimary (citable) accession number: Q6GFE4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: July 19, 2004
Last modified: June 7, 2017
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families