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Protein

NH(3)-dependent NAD(+) synthetase

Gene

nadE

Organism
Staphylococcus aureus (strain MRSA252)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. NH(3)-dependent NAD(+) synthetase (nadE)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei49 – 491UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi47 – 548ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAUR282458:GJA5-2037-MONOMER.
UniPathwayiUPA00253; UER00333.

Names & Taxonomyi

Protein namesi
Recommended name:
NH(3)-dependent NAD(+) synthetaseUniRule annotation (EC:6.3.1.5UniRule annotation)
Gene namesi
Name:nadEUniRule annotation
Ordered Locus Names:SAR2005
OrganismiStaphylococcus aureus (strain MRSA252)
Taxonomic identifieri282458 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 273273NH(3)-dependent NAD(+) synthetasePRO_0000152197Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ6GFE4.
SMRiQ6GFE4. Positions 4-268.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD synthetase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000238070.
KOiK01916.
OMAiDFVRGNI.
OrthoDBiEOG64JFM7.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6GFE4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKLQDVIVQ EMKVKKRIDS AEEIAELKQF IKSYVQSHSF IKSLVLGISG
60 70 80 90 100
GQDSTLVGKL VQMSVNELRE EGIDCTFIAV KLPYGVQKDA DEVEQALQFI
110 120 130 140 150
EPDEIVTVNI KPAVDQSVQS LKEAGIVLTD FQKGNEKARE RMKVQFSIAS
160 170 180 190 200
NRQGIVVGTD HSAENITGFY TKYGDGAADI APIFGLNKRQ GRQLLAYLGA
210 220 230 240 250
PKQLYEKTPT ADLEDDKPQL PDEDALGVTY EAIDNYLEGK PVTPEEQKVI
260 270
ENHYIRNAHK RELAYTRYTW PKS
Length:273
Mass (Da):30,581
Last modified:July 19, 2004 - v1
Checksum:iA369E3B1D6F6EFA5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571856 Genomic DNA. Translation: CAG40990.1.
RefSeqiWP_000040861.1. NC_002952.2.

Genome annotation databases

EnsemblBacteriaiCAG40990; CAG40990; SAR2005.
KEGGisar:SAR2005.
PATRICi19547653. VBIStaAur71814_2017.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571856 Genomic DNA. Translation: CAG40990.1.
RefSeqiWP_000040861.1. NC_002952.2.

3D structure databases

ProteinModelPortaliQ6GFE4.
SMRiQ6GFE4. Positions 4-268.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAG40990; CAG40990; SAR2005.
KEGGisar:SAR2005.
PATRICi19547653. VBIStaAur71814_2017.

Phylogenomic databases

HOGENOMiHOG000238070.
KOiK01916.
OMAiDFVRGNI.
OrthoDBiEOG64JFM7.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00333.
BioCyciSAUR282458:GJA5-2037-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MRSA252.

Entry informationi

Entry nameiNADE_STAAR
AccessioniPrimary (citable) accession number: Q6GFE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: July 19, 2004
Last modified: March 16, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.