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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Staphylococcus aureus (strain MRSA252)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39Substrate; via amide nitrogenUniRule annotation1
Binding sitei115ATPUniRule annotation1
Binding sitei160ATPUniRule annotation1
Binding sitei187Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:SAR2181
OrganismiStaphylococcus aureus (strain MRSA252)
Taxonomic identifieri282458 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001569551 – 263Hydroxyethylthiazole kinaseAdd BLAST263

Structurei

Secondary structure

1263
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 10Combined sources9
Beta strandi13 – 17Combined sources5
Turni20 – 22Combined sources3
Helixi23 – 32Combined sources10
Beta strandi36 – 38Combined sources3
Helixi43 – 52Combined sources10
Beta strandi54 – 59Combined sources6
Helixi65 – 81Combined sources17
Beta strandi85 – 88Combined sources4
Helixi92 – 94Combined sources3
Helixi96 – 108Combined sources13
Beta strandi112 – 117Combined sources6
Helixi118 – 125Combined sources8
Beta strandi126 – 128Combined sources3
Beta strandi132 – 134Combined sources3
Beta strandi136 – 138Combined sources3
Helixi142 – 153Combined sources12
Beta strandi155 – 168Combined sources14
Beta strandi171 – 175Combined sources5
Helixi180 – 184Combined sources5
Helixi188 – 198Combined sources11
Helixi208 – 227Combined sources20
Helixi234 – 247Combined sources14
Helixi250 – 256Combined sources7
Beta strandi259 – 263Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5CGAX-ray1.87A/B/C/E/F/H1-263[»]
5CGEX-ray1.62A/B/C/D/E/F1-263[»]
5CM5X-ray2.09A/B/C/D/E/F1-263[»]
5COJX-ray1.90A/B/C/E/F/H1-263[»]
ProteinModelPortaliQ6GEY3.
SMRiQ6GEY3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000114352.
KOiK00878.
OMAiSPVMAHA.

Family and domain databases

CDDicd01170. THZ_kinase. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase. 1 hit.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6GEY3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYLNNIRIE NPLTICYTND VVKNFTANGL LSIGASPAMS EAPEEAEEFY
60 70 80 90 100
KVAQALLINI GTLTAQNEQD IIAIAQTANE AGLPIVFDPV AVGASTYRKQ
110 120 130 140 150
FCKLLLKSAK VSVIKGNASE ILALIDDTAT MKGTDSDANL DAVTIAKKAY
160 170 180 190 200
AIYKTAIVIT GKEDVIVQGD KAIVLANGSP LLARVTGAGC LLGGIIAGFL
210 220 230 240 250
FRETEPDIEA LIEAVSVFNI AAEVAAENEN CGGPGTFSPL LLDTLYHLNE
260
TTYQQRIRIQ EVE
Length:263
Mass (Da):28,040
Last modified:July 19, 2004 - v1
Checksum:i6072DA60FF96F1AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571856 Genomic DNA. Translation: CAG41161.1.
RefSeqiWP_001108496.1. NC_002952.2.

Genome annotation databases

EnsemblBacteriaiCAG41161; CAG41161; SAR2181.
KEGGisar:SAR2181.
PATRICi19547999. VBIStaAur71814_2185.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571856 Genomic DNA. Translation: CAG41161.1.
RefSeqiWP_001108496.1. NC_002952.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5CGAX-ray1.87A/B/C/E/F/H1-263[»]
5CGEX-ray1.62A/B/C/D/E/F1-263[»]
5CM5X-ray2.09A/B/C/D/E/F1-263[»]
5COJX-ray1.90A/B/C/E/F/H1-263[»]
ProteinModelPortaliQ6GEY3.
SMRiQ6GEY3.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAG41161; CAG41161; SAR2181.
KEGGisar:SAR2181.
PATRICi19547999. VBIStaAur71814_2185.

Phylogenomic databases

HOGENOMiHOG000114352.
KOiK00878.
OMAiSPVMAHA.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.

Family and domain databases

CDDicd01170. THZ_kinase. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase. 1 hit.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHIM_STAAR
AccessioniPrimary (citable) accession number: Q6GEY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: July 19, 2004
Last modified: November 2, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.