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Q6GEN9 (PTLCB_STAAR) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
PTS system lactose-specific EIICB component
Alternative name(s):
EIICB-Lac
Short name=EII-Lac

Including the following 2 domains:

  1. Lactose permease IIC component
    Alternative name(s):
    PTS system lactose-specific EIIC component
  2. Lactose-specific phosphotransferase enzyme IIB component
    EC=2.7.1.69
    Alternative name(s):
    PTS system lactose-specific EIIB component
Gene names
Name:lacE
Ordered Locus Names:SAR2281
OrganismStaphylococcus aureus (strain MRSA252) [Complete proteome] [HAMAP]
Taxonomic identifier282458 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length570 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in lactose transport.

Catalytic activity

Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.

Subcellular location

Cell membrane; Multi-pass membrane protein Probable.

Domain

The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.

Sequence similarities

Contains 1 PTS EIIB type-3 domain.

Contains 1 PTS EIIC type-3 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 570570PTS system lactose-specific EIICB component
PRO_0000186588

Regions

Transmembrane30 – 5021Helical; Potential
Transmembrane61 – 8121Helical; Potential
Transmembrane102 – 12221Helical; Potential
Transmembrane131 – 15121Helical; Potential
Transmembrane174 – 19421Helical; Potential
Transmembrane219 – 23921Helical; Potential
Transmembrane278 – 29821Helical; Potential
Transmembrane334 – 35421Helical; Potential
Transmembrane375 – 39521Helical; Potential
Domain1 – 410410PTS EIIC type-3
Domain467 – 570104PTS EIIB type-3

Sites

Active site4741Phosphocysteine intermediate; for EIIB activity By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6GEN9 [UniParc].

Last modified July 19, 2004. Version 1.
Checksum: 587192287079BE58

FASTA57062,432
        10         20         30         40         50         60 
MMQKLIAQIE KGKPFFEKLS RNIYLRAIRD GFISAMPVIL FSSIFLLIAY VPNIFGFKWD 

        70         80         90        100        110        120 
KGMEAILMKP YNYTMGLVAF LVAGTTAKSL TDSFNRKLES TNQINFISTM LAAMCGFLFL 

       130        140        150        160        170        180 
ASDPAKDGGF LSAFMGTKGL LTAFLSAFVT VIVYNFCVKR NITIKMPKEV PPNISQVFKD 

       190        200        210        220        230        240 
LIPFSAVIII LYALDLVIRN SFKSNVAEGI LKLFEPLFTA ADGWIGVTII FGAFALFWFV 

       250        260        270        280        290        300 
GIHGPSIVEP AIAAITYANI EANFKLLQAG EHADKIITSG TQMFIVTFGG TGATLVVPFM 

       310        320        330        340        350        360 
FMWMTKSKRN KAIGRASVVP TFFGVNEPIL FGAPLVLNPV FFIPFVLAPI VNVWIFKLFV 

       370        380        390        400        410        420 
EVLGMNSFSV NLPWTTPGPL GIIMGTGFGL WSFVLAITLI VVDIIIYYPF LKVYDSEILD 

       430        440        450        460        470        480 
EEEGRKESNS DLKEKVAANF DTKKADSILA ASGVSDDAAK ASNITEQTNV LVLCAGGGTS 

       490        500        510        520        530        540 
GLLANALNKA AEEYHVPVKA AAGGYGAHMD IMKEYQLIIL APQVASNYED IKQDTDRLGI 

       550        560        570 
KLAKTQGAEY IKLTRDGQAA LDFVQQQFEN

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX571856 Genomic DNA. Translation: CAG41259.1.
RefSeqYP_041634.1. NC_002952.2.

3D structure databases

ProteinModelPortalQ6GEN9.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ6GEN9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBSTAT00000020386; EBSTAP00000019722; EBSTAG00000020385.
GeneID2859691.
GenomeReviewsGene locus SAR2281 in contig BX571856_GR.
KEGGsar:SAR2281.
PATRIC19548229. VBIStaAur71814_2291.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1455.
GeneTreeEBGT00050000025328.
HOGENOMHBG337916.
OMAWSKDIEN.
ProtClustDBCLSK877288.

Enzyme and pathway databases

BioCycSAUR282458:SAR2281-MONOMER.

Family and domain databases

InterProIPR014350. PTrfase_system_EIIB_3_subgr.
IPR003501. PTS_EIIB_2/3.
IPR013012. PTS_EIIB_3.
IPR003352. PTS_EIIC.
IPR004501. PTS_IIC_lac.
IPR004801. PTS_IIC_lac_spec.
[Graphical view]
KOK02787.
K02788.
PfamPF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
[Graphical view]
TIGRFAMsTIGR00394. Lac_pts_IIC. 1 hit.
TIGR00410. LacE. 1 hit.
TIGR00853. Pts-lac. 1 hit.
PROSITEPS51100. PTS_EIIB_TYPE_3. 1 hit.
PS51105. PTS_EIIC_TYPE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePTLCB_STAAR
AccessionPrimary (citable) accession number: Q6GEN9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 19, 2004
Last modified: January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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