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Protein

DNA topoisomerase 3

Gene

topB

Organism
Staphylococcus aureus (strain MRSA252)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi8 – 81Magnesium; catalyticUniRule annotation
Sitei60 – 601Interaction with DNAUniRule annotation
Metal bindingi104 – 1041Magnesium; catalyticUniRule annotation
Sitei167 – 1671Interaction with DNAUniRule annotation
Sitei175 – 1751Interaction with DNAUniRule annotation
Active sitei305 – 3051O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
Sitei307 – 3071Interaction with DNAUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSAUR282458:GJA5-2382-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IIIUniRule annotation
Gene namesi
Name:topBUniRule annotation
Ordered Locus Names:SAR2339
OrganismiStaphylococcus aureus (strain MRSA252)
Taxonomic identifieri282458 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 711711DNA topoisomerase 3PRO_0000286373Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ6GEH9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 135134ToprimUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni186 – 1916Interaction with DNAUniRule annotation

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0551.
HOGENOMiHOG000086847.
KOiK03169.
OMAiTKHDEYF.
OrthoDBiEOG60CWM6.

Family and domain databases

Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00953. Topoisom_3_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01056. topB. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6GEH9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSLILAEKP SVARDIADAL QINQKRNGYF ENNQYIVTWA LGHLVTNATP
60 70 80 90 100
EQYDKNLKEW RLEDLPIIPK YMKTVVIGKT SKQFKTVKAL ILDNKVKDII
110 120 130 140 150
IATDAGREGE LVARLILDKV GNKKPLRRLW ISSVTKKAIQ QGFKNLKDGR
160 170 180 190 200
QYNDLYYAAL ARSEADWIVG INATRALTTK YDAQLSLGRV QTPTIQLVNT
210 220 230 240 250
RQQEINQFKP QQYYTLSLTV KGFDFQLESN QRYTNKETLE QIVNNLKNVD
260 270 280 290 300
GKIKSVATKH KKSYPQSLYN LTDLQQDMYR RYKIGPKETL NTLQSLYERH
310 320 330 340 350
KVVTYPRTDS NYLTTDMVDT MKERIQATMA TTYKDQARPL MSKTFSSKMS
360 370 380 390 400
IFNNQKVSDH HAIIPTEVRP VMSDLSNREL KLYDMIVERF LEALMSPHEY
410 420 430 440 450
DAITVTLEVA GHTFVLKENV TTVLGFKSIR QGESITEMQQ PFSEGDEVKI
460 470 480 490 500
SKTNIREHET TPPEYFNEGS LLKAMENPQN FIQLKDKKYA QTLKQTGGIG
510 520 530 540 550
TVATRADIID KLFNMNAIES RDGKIKVTSK GKQILELAPE ELTSPLLTAQ
560 570 580 590 600
WEEKLLLIER GKYQAKTFIN EMKDFTKDVV NGIKNSDRKY KHDNLTTTEC
610 620 630 640 650
PTCGKFMIKV KTKNGQMLVC QDPSCKTKKN VQRKTNARCP NCKKKLTLFG
660 670 680 690 700
KGKEAVYRCV CGHSETQAHM DQRMKSKSSG KVSRKEMKKY MNKNEGLDNN
710
PFKDALKNLN L
Length:711
Mass (Da):81,512
Last modified:July 19, 2004 - v1
Checksum:i69977AAAB6BF6758
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571856 Genomic DNA. Translation: CAG41320.1.
RefSeqiWP_000838499.1. NC_002952.2.

Genome annotation databases

EnsemblBacteriaiCAG41320; CAG41320; SAR2339.
KEGGisar:SAR2339.
PATRICi19548349. VBIStaAur71814_2351.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571856 Genomic DNA. Translation: CAG41320.1.
RefSeqiWP_000838499.1. NC_002952.2.

3D structure databases

ProteinModelPortaliQ6GEH9.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAG41320; CAG41320; SAR2339.
KEGGisar:SAR2339.
PATRICi19548349. VBIStaAur71814_2351.

Phylogenomic databases

eggNOGiCOG0551.
HOGENOMiHOG000086847.
KOiK03169.
OMAiTKHDEYF.
OrthoDBiEOG60CWM6.

Enzyme and pathway databases

BioCyciSAUR282458:GJA5-2382-MONOMER.

Family and domain databases

Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00953. Topoisom_3_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01056. topB. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MRSA252.

Entry informationi

Entry nameiTOP3_STAAR
AccessioniPrimary (citable) accession number: Q6GEH9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: July 19, 2004
Last modified: July 22, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.