Q6GCP9 (PFLA_STAAS) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 50.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Pyruvate formate-lyase-activating enzyme Short name=PFL-activating enzyme EC=1.97.1.4 | ||||
| Gene names |
| ||||
| Organism | Staphylococcus aureus (strain MSSA476) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 282459 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Firmicutes › Bacillales › Staphylococcus |
Protein attributes
| Sequence length | 251 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine By similarity. |
| Catalytic activity | S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical. |
| Cofactor | Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. |
| Subcellular location | Cytoplasm By similarity. |
| Sequence similarities | Belongs to the organic radical-activating enzymes family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Carbohydrate metabolism Glucose metabolism |
| Cellular component | Cytoplasm |
| Ligand | 4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | glucose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW [formate-C-acetyltransferase]-activating enzyme activityInferred from electronic annotation. Source: EC metal ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 251 | 251 | Pyruvate formate-lyase-activating enzyme | PRO_0000271715 | |||||
Sites | |||||||||
| Metal binding | 29 | 1 | Iron-sulfur (4Fe-4S-S-AdoMet) By similarity | ||||||
| Metal binding | 33 | 1 | Iron-sulfur (4Fe-4S-S-AdoMet) By similarity | ||||||
| Metal binding | 36 | 1 | Iron-sulfur (4Fe-4S-S-AdoMet) By similarity | ||||||
Sequences
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References
| [1] | "Complete genomes of two clinical Staphylococcus aureus strains: evidence for the rapid evolution of virulence and drug resistance." Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J., Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A., Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C., Clark L., Corton C. Parkhill J.Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004) [PubMed: 15213324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MSSA476. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | BX571857 Genomic DNA. Translation: CAG41970.1. |
| RefSeq | YP_042324.1. NC_002953.3. |
3D structure databases | |
| ProteinModelPortal | Q6GCP9. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q6GCP9. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | EBSTAT00000022896; EBSTAP00000022063; EBSTAG00000022895. |
| GeneID | 2864326. |
| GenomeReviews | Gene locus SAS0202 in contig BX571857_GR. |
| KEGG | sas:SAS0202. |
| PATRIC | 19549750. VBIStaAur96780_0213. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| eggNOG | COG1180. |
| GeneTree | EBGT00050000024170. |
| HOGENOM | HBG554871. |
| OMA | LIGVPNK. |
| ProtClustDB | CLSK884495. |
Enzyme and pathway databases | |
| BioCyc | SAUR282459:SAS0202-MONOMER. |
Family and domain databases | |
| InterPro | IPR012838. PFL_activating. IPR001989. Radical_activat_CS. IPR007197. rSAM. [Graphical view] |
| KO | K04069. |
| Pfam | PF04055. Radical_SAM. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR02493. PFLA. 1 hit. |
| PROSITE | PS01087. RADICAL_ACTIVATING. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | PFLA_STAAS | ||||||||
| Accession | Primary (citable) accession number: Q6GCP9 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |

Clusters with