Reviewed, UniProtKB/Swiss-Prot Q6GCM3 (ISPD1_STAAS)

Names and origin

Protein names

Recommended name:
    2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1
    EC=2.7.7.60
Alternative name(s):
    4-diphosphocytidyl-2C-methyl-D-erythritol synthase 1
    MEP cytidylyltransferase 1
      Short name=MCT 1

Gene names

Name:	ispD1
Ordered Locus Names:	SAS0227

Organism

Staphylococcus aureus (strain MSSA476) [Complete proteome] [HAMAP]

Taxonomic identifier

282459 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity.
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl-PP biosynthesis via DXP pathway; isopentenyl-PP from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the ispD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1 HAMAP MF_00108		PRO_0000075621
Sites
	Site	14	1	Transition state stabilizer By similarity
	Site	22	1	Transition state stabilizer By similarity
	Site	160	1	Positions MEP for the nucleophilic attack By similarity
	Site	217	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6GCM3-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: F21440DA33D18AA7	FASTA	238	26,627
	10 20 30 40 50 60 MIYAGILAGG IGSRMGNVPL PKQFLDIDNK PILIHTIEKF ILVSEFNEII IATPAQWISH 70 80 90 100 110 120 TQDILKKYNI TDQRVKVVAG GTDRNETIMN IIDYIRNVNG INNDDVIVTH DAVRPFLTQR 130 140 150 160 170 180 IIKENIEVAE KYGAVDTVIE AIDTIVMSKD KQNIHSIPVR NEMYQGQTPQ SFNIKLLQDS 190 200 210 220 230 YRALSSAQKE ILSDACKIIV ESGHPVKLVR GELYNIKVTT PYDLKVANAI IQGDIADD

References

[1]

"Complete genomes of two clinical Staphylococcus aureus strains: evidence for the rapid evolution of virulence and drug resistance." Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J., Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A., Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C., Clark L., Corton C. , Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z., Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N., Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M., Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004) [PubMed: 15213324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX571857 Genomic DNA. Translation: CAG41996.1.
RefSeq	YP_042350.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2861974.
GenomeReviews	Gene locus SAS0227 in contig BX571857_GR.
KEGG	sas:SAS0227.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6GCM3.
OMA	Q6GCM3. GELFNIK.
Enzyme and pathway databases
BioCyc	SAUR282459:SAS0227-MON.
Family and domain databases
HAMAP	MF_00108. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
Pfam	PF01128. IspD. 1 hit. [Graphical view]
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD1_STAAS

Accession

Primary (citable) accession number: Q6GCM3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	July 19, 2004
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)