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Protein

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1

Gene

ispD1

Organism
Staphylococcus aureus (strain MSSA476)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP).By similarity

Catalytic activityi

CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 2 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1 (ispD1), 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2 (ispD2)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. no protein annotated in this organism
  5. no protein annotated in this organism
  6. no protein annotated in this organism
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei14 – 141Transition state stabilizerBy similarity
Sitei22 – 221Transition state stabilizerBy similarity
Sitei160 – 1601Positions MEP for the nucleophilic attackBy similarity
Sitei217 – 2171Positions MEP for the nucleophilic attackBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Isoprene biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00056; UER00093.

Names & Taxonomyi

Protein namesi
Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1 (EC:2.7.7.60)
Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase 1
MEP cytidylyltransferase 1
Short name:
MCT 1
Gene namesi
Name:ispD1
Ordered Locus Names:SAS0227
OrganismiStaphylococcus aureus (strain MSSA476)
Taxonomic identifieri282459 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2382382-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1PRO_0000075621Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ6GCM3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IspD family.Curated

Phylogenomic databases

HOGENOMiHOG000218563.
KOiK00991.
OMAiNDRFDKI.
OrthoDBiEOG6FFS8T.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_00108. IspD.
InterProiIPR001228. IspD.
IPR018294. ISPD_synthase_CS.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01128. IspD. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiPS01295. ISPD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6GCM3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIYAGILAGG IGSRMGNVPL PKQFLDIDNK PILIHTIEKF ILVSEFNEII
60 70 80 90 100
IATPAQWISH TQDILKKYNI TDQRVKVVAG GTDRNETIMN IIDYIRNVNG
110 120 130 140 150
INNDDVIVTH DAVRPFLTQR IIKENIEVAE KYGAVDTVIE AIDTIVMSKD
160 170 180 190 200
KQNIHSIPVR NEMYQGQTPQ SFNIKLLQDS YRALSSAQKE ILSDACKIIV
210 220 230
ESGHPVKLVR GELYNIKVTT PYDLKVANAI IQGDIADD
Length:238
Mass (Da):26,627
Last modified:July 19, 2004 - v1
Checksum:iF21440DA33D18AA7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571857 Genomic DNA. Translation: CAG41996.1.
RefSeqiWP_000638479.1. NC_002953.3.

Genome annotation databases

KEGGisas:SAS0227.
PATRICi19549802. VBIStaAur96780_0239.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571857 Genomic DNA. Translation: CAG41996.1.
RefSeqiWP_000638479.1. NC_002953.3.

3D structure databases

ProteinModelPortaliQ6GCM3.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGisas:SAS0227.
PATRICi19549802. VBIStaAur96780_0239.

Phylogenomic databases

HOGENOMiHOG000218563.
KOiK00991.
OMAiNDRFDKI.
OrthoDBiEOG6FFS8T.

Enzyme and pathway databases

UniPathwayiUPA00056; UER00093.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_00108. IspD.
InterProiIPR001228. IspD.
IPR018294. ISPD_synthase_CS.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01128. IspD. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiPS01295. ISPD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MSSA476.

Entry informationi

Entry nameiISPD1_STAAS
AccessioniPrimary (citable) accession number: Q6GCM3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: July 19, 2004
Last modified: March 16, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.