Q6GCL7 (ISPD2_STAAS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase 2
MEP cytidylyltransferase 2
Short name=MCT 2

Gene names

Name:	ispD2
Ordered Locus Names:	SAS0232

Organism

Staphylococcus aureus (strain MSSA476) [Complete proteome] [HAMAP]

Taxonomic identifier

282459 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2 HAMAP MF_00108		PRO_0000075622
Sites
	Site	14	1	Transition state stabilizer By similarity
	Site	22	1	Transition state stabilizer By similarity
	Site	160	1	Positions MEP for the nucleophilic attack By similarity
	Site	217	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6GCL7 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: B19F7EA82D847A0F	FASTA	238	26,657
	10 20 30 40 50 60 MKYAGILAGG IGSRMGNVPL PKQFLDLDNK PILIHTLEKF ILINDFEKII IATPQQWMTH 70 80 90 100 110 120 TKDTLRKFKI SDERIEVIQG GSDRNDTIMN IVKHIESTNG INDDDVIVTH DAVRPFLTHR 130 140 150 160 170 180 IIKENIQAAL EYGAVDTVID AIDTIVTSKD NQTIDAIPVR NEMYQGQTPQ SFNINLLKES 190 200 210 220 230 YAQLSDEQKS ILSDACKIIV ETNKPVRLVK GELYNIKVTT PYDLKVANAI IRGGIADD

References

[1]

"Complete genomes of two clinical Staphylococcus aureus strains: evidence for the rapid evolution of virulence and drug resistance." Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J., Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A., Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C., Clark L., Corton C. , Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z., Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N., Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M., Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004) [PubMed: 15213324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MSSA476.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571857 Genomic DNA. Translation: CAG42002.1.
RefSeq	YP_042356.1. NC_002953.3.
3D structure databases
ProteinModelPortal	Q6GCL7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6GCL7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000022380; EBSTAP00000021547; EBSTAG00000022379.
GeneID	2862546.
GenomeReviews	Gene locus SAS0232 in contig BX571857_GR.
KEGG	sas:SAS0232.
PATRIC	19549814. VBIStaAur96780_0245.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1211.
GeneTree	EBGT00050000024510.
HOGENOM	HBG672839.
OMA	REQQDFW.
PhylomeDB	Q6GCL7.
ProtClustDB	PRK00155.
Enzyme and pathway databases
BioCyc	SAUR282459:SAS0232-MONOMER.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD2_STAAS

Accession

Primary (citable) accession number: Q6GCL7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	July 19, 2004
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program