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Protein

Hypoxanthine-guanine phosphoribosyltransferase

Gene

hpt

Organism
Staphylococcus aureus (strain MSSA476)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein.By similarity

Pathway: IMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes IMP from hypoxanthine.
Proteins known to be involved in this subpathway in this organism are:
  1. Hypoxanthine-guanine phosphoribosyltransferase (hpt)
This subpathway is part of the pathway IMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from hypoxanthine, the pathway IMP biosynthesis via salvage pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei102 – 1021Proton acceptorBy similarity
Binding sitei130 – 1301IMPBy similarity
Binding sitei152 – 1521IMP; via carbonyl oxygenBy similarity
Metal bindingi158 – 1581MagnesiumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi98 – 10710IMPBy similarity
Nucleotide bindingi157 – 1582IMPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00591; UER00648.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HGPRT
Short name:
HGPRTase
Gene namesi
Name:hpt
Ordered Locus Names:SAS0467
OrganismiStaphylococcus aureus (strain MSSA476)
Taxonomic identifieri282459 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 179179Hypoxanthine-guanine phosphoribosyltransferasePRO_0000139616Add
BLAST

Proteomic databases

PRIDEiQ6GBX8.

Structurei

3D structure databases

ProteinModelPortaliQ6GBX8.
SMRiQ6GBX8. Positions 1-179.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0634.
HOGENOMiHOG000236520.
KOiK00760.
OMAiTMDWMAV.
OrthoDBiEOG693GNP.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6GBX8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHNDLKEVLL TEEDIQNICK ELGAQLTKDY QGKPLVCVGI LKGSAMFMSD
60 70 80 90 100
LIKRIDTHLS IDFMDVSSYH GGTESTGEVQ IIKDLGSSIE NKDVLIIEDI
110 120 130 140 150
LETGTTLKSI TELLQSRKVN SLEIVTLLDK PNRRKADIEA KYVGKKIPDE
160 170
FVVGYGLDYR ELYRNLPYIG TLKPEVYSN
Length:179
Mass (Da):20,154
Last modified:July 19, 2004 - v1
Checksum:iE7D35987B435EFB0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571857 Genomic DNA. Translation: CAG42242.1.
RefSeqiWP_000551283.1. NC_002953.3.
YP_042595.1. NC_002953.3.

Genome annotation databases

GeneIDi23196367.
KEGGisas:SAS0467.
PATRICi19550308. VBIStaAur96780_0488.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571857 Genomic DNA. Translation: CAG42242.1.
RefSeqiWP_000551283.1. NC_002953.3.
YP_042595.1. NC_002953.3.

3D structure databases

ProteinModelPortaliQ6GBX8.
SMRiQ6GBX8. Positions 1-179.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ6GBX8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi23196367.
KEGGisas:SAS0467.
PATRICi19550308. VBIStaAur96780_0488.

Phylogenomic databases

eggNOGiCOG0634.
HOGENOMiHOG000236520.
KOiK00760.
OMAiTMDWMAV.
OrthoDBiEOG693GNP.

Enzyme and pathway databases

UniPathwayiUPA00591; UER00648.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MSSA476.

Entry informationi

Entry nameiHPRT_STAAS
AccessioniPrimary (citable) accession number: Q6GBX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: July 19, 2004
Last modified: June 24, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.