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Protein

D-alanine--D-alanyl carrier protein ligase

Gene

dltA

Organism
Staphylococcus aureus (strain MSSA476)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the first step in the D-alanylation of lipoteichoic acid (LTA), the activation of D-alanine and its transfer onto the D-alanyl carrier protein (Dcp) DltC. In an ATP-dependent two-step reaction, forms a high energy D-alanyl-AMP intermediate, followed by transfer of the D-alanyl residue as a thiol ester to the phosphopantheinyl prosthetic group of the Dcp. D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall.UniRule annotation

Catalytic activityi

D-alanine + ATP + holo-[D-alanyl-carrier protein] = AMP + diphosphate + D-alanyl-[D-alanyl-carrier protein].UniRule annotation

Pathwayi: lipoteichoic acid biosynthesis

This protein is involved in the pathway lipoteichoic acid biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway lipoteichoic acid biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei189D-alanineUniRule annotation1
Binding sitei293D-alanine; via carbonyl oxygenUniRule annotation1
Binding sitei365ATPUniRule annotation1
Binding sitei473ATPUniRule annotation1
Binding sitei473D-alanineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi144 – 145ATPUniRule annotation2
Nucleotide bindingi284 – 289ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00556.

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanyl carrier protein ligaseUniRule annotation (EC:6.2.1.-UniRule annotation)
Short name:
DCLUniRule annotation
Alternative name(s):
D-alanine--poly(phosphoribitol) ligase subunit 1UniRule annotation
D-alanine-activating enzymeUniRule annotation
Short name:
DAEUniRule annotation
Gene namesi
Name:dltAUniRule annotation
Ordered Locus Names:SAS0802
OrganismiStaphylococcus aureus (strain MSSA476)
Taxonomic identifieri282459 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002131541 – 485D-alanine--D-alanyl carrier protein ligaseAdd BLAST485

Structurei

3D structure databases

ProteinModelPortaliQ6GAZ4.
SMRiQ6GAZ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent AMP-binding enzyme family. DltA subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000229995.
KOiK03367.
OMAiNFYIIFT.

Family and domain databases

HAMAPiMF_00593. DltA. 1 hit.
InterProiView protein in InterPro
IPR010071. AA_adenyl_domain.
IPR025110. AMP-bd_C.
IPR000873. AMP-dep_Synth/Lig.
IPR010072. DltA.
PfamiView protein in Pfam
PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
TIGRFAMsiTIGR01733. AA-adenyl-dom. 1 hit.
TIGR01734. D-ala-DACP-lig. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6GAZ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDIINKLQA FADANPQSIA VRHTTDELTY QQLMDESSKL AHRLQGSKKP
60 70 80 90 100
MILFGHMSPY MIVGMIGAIK AGCGYVPVDT SIPEDRIKMI INKVQPEFVF
110 120 130 140 150
NTTDESFESL EGEVFTIEDI KTSQDPVIFD SQIKDNDTVY TIFTSGSTGE
160 170 180 190 200
PKGVQIEYAS LVQFTEWMLE LNKSGNEQQW LNQAPFSFDL SVMAIYPCLA
210 220 230 240 250
SGGTLNLVDK NMINKPKLLN EMLTATPINI WVSTPSFMEM CLLLPTLNEE
260 270 280 290 300
QYGSLNEFFF CGEILPHRAA KALVNRFPSA TIYNTYGPTE ATVAVTSIQI
310 320 330 340 350
TQEILDQYPT LPVGVERPGA RLSTTDEGEL VIEGQSVSLG YLKNDQKTAE
360 370 380 390 400
VFNFDDGIRT YHTGDKAKFE NGQWFIQGRI DFQIKLNGYR MELEEIETQL
410 420 430 440 450
RQSEFVKEAI VVPVYKNDKV IHLIGAIVPT TEVTDNAEMT KNIKNDLKSR
460 470 480
LPEYMIPRKF EWMEQLPLTS NGKIDRKKIA EVING
Length:485
Mass (Da):54,670
Last modified:July 19, 2004 - v1
Checksum:iFFA535607F1FD328
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571857 Genomic DNA. Translation: CAG42577.1.
RefSeqiWP_000129653.1. NC_002953.3.

Genome annotation databases

KEGGisas:SAS0802.

Similar proteinsi

Entry informationi

Entry nameiDLTA_STAAS
AccessioniPrimary (citable) accession number: Q6GAZ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: July 19, 2004
Last modified: December 20, 2017
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families