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Q6GAG0

- ATL_STAAS

UniProt

Q6GAG0 - ATL_STAAS

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Protein

Bifunctional autolysin

Gene
atl, nag, SAS0988
Organism
Staphylococcus aureus (strain MSSA476)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Endohydrolysis of the di-N-acetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -[(Man)5(GlcNAc)2]-Asn structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact. Cleaves the peptidoglycan connecting the daughter cells at the end of the cell division cycle, resulting in the separation of the two newly divided cells. Acts as an autolysin in penicillin-induced lysis By similarity.

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact.

GO - Molecular functioni

  1. amidase activity Source: InterPro
  2. mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity Source: UniProtKB-EC
  3. N-acetylmuramoyl-L-alanine amidase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell wall macromolecule metabolic process Source: InterPro
  2. peptidoglycan catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Protein family/group databases

CAZyiGH73. Glycoside Hydrolase Family 73.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional autolysin
Including the following 2 domains:
N-acetylmuramoyl-L-alanine amidase (EC:3.5.1.28)
Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (EC:3.2.1.96)
Gene namesi
Name:atl
Synonyms:nag
Ordered Locus Names:SAS0988
OrganismiStaphylococcus aureus (strain MSSA476)
Taxonomic identifieri282459 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000002201: Chromosome

Subcellular locationi

Secreted By similarity
Note: Secreted, and then anchored on the cell surface at the peripheral cell wall above the completed septum (septal region), for the next cell division cycle By similarity.

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929 Reviewed predictionAdd
BLAST
Chaini30 – 12501221Bifunctional autolysinPRO_0000045477Add
BLAST

Post-translational modificationi

Undergoes proteolytic processing to generate the two extracellular lytic enzymes, probably at the septal region on the cell surface By similarity.

Interactioni

Subunit structurei

Oligomer; forms a ring structure at the cell surface which is important for efficient partitioning of daughter cells after cell division By similarity.

Protein-protein interaction databases

STRINGi282459.SAS0988.

Structurei

3D structure databases

ProteinModelPortaliQ6GAG0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati425 – 5831591Add
BLAST
Repeati590 – 7521632Add
BLAST
Repeati764 – 9261633Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni199 – 769571N-acetylmuramoyl-L-alanine amidaseAdd
BLAST
Regioni770 – 1250481Endo-beta-N-acetylglucosaminidaseAdd
BLAST

Domaini

The repeat domains R1, R2 and R3 are responsible for directing the proteins to the septal region By similarity.

Sequence similaritiesi

In the N-terminal section; belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.
In the C-terminal section; belongs to the glycosyl hydrolase 73 family.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG4193.
HOGENOMiHOG000279968.
KOiK13714.
OMAiKSGWISK.
OrthoDBiEOG6GXTN7.

Family and domain databases

Gene3Di3.40.80.10. 1 hit.
InterProiIPR002502. Amidase_domain.
IPR013338. Lysozyme_subfam2_dom.
IPR002901. MGlyc_endo_b_GlcNAc_like_dom.
[Graphical view]
PfamiPF01510. Amidase_2. 1 hit.
PF01832. Glucosaminidase. 1 hit.
[Graphical view]
SMARTiSM00644. Ami_2. 1 hit.
SM00047. LYZ2. 1 hit.
[Graphical view]
SUPFAMiSSF55846. SSF55846. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6GAG0-1 [UniParc]FASTAAdd to Basket

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MAKKFNYKLP SMVALTLVGS AVTAHQVQAA ETTQDQTTNK NVLDSNKVKA     50
TTEQAKAEVK NPTQNISGTQ VYQDPAIVQP KTANNKTGNA QVSQKVDTAQ 100
VNGDTRANQS ATTNNTQPVA KSTSTTAPKT NTNVTNAGYS LVDDEDDNSE 150
NQINPELIKS AAKPAALETQ YKAAAPKAAT TSAPKAKTEA TPKVTTFSAS 200
AQPRSVAATP KTSLPKYKPQ VNSSINDYIR KNNLKAPKIE EDYTSYFPKY 250
AYRNGVGRPE GIVVHDTAND RSTINGEISY MKNNYQNAFV HAFVDGDRII 300
ETAPTDYLSW GVGAVGNPRF INVEIVHTHD YASFARSMNN YADYAATQLQ 350
YYGLKPDSAE YDGNGTVWTH YAVSKYLGGT DHADPHGYLR SHNYSYDQLY 400
DLINEKYLIK MGKVAPWGTQ STTTPTTPSK PSTGKLTVAA NNGVAQIKPT 450
NSGLYTTVYD KTGKATNEVQ KTFAVSKTAT LGNQKFYLVQ DYNSGNKFGW 500
VKEGDVVYNT AKSPVNVNQS YSIKPGTKLY TVPWGTSKQV AGSVSGSGNQ 550
TFKASKQQQI DKSIYLYGSV NGKSGWVSKA YLVDTAKPTP TPTPKPSTPT 600
TNNKLTVSSL NGVAQINAKN NGLFTTVYDK TGKPTKEVQK TFAVTKEASL 650
GGNKFYLVKD YNSPTLIGWV KQGDVIYNNA KSPVNVMQTY TVKPGTKLYS 700
VPWGTYKQEA GAVSGTGNQT FKATKQQQID KSIYLFGTVN GKSGWVSKAY 750
LAVPAAPKKA VAQPKTAVKA YTVTKPQTTQ TVSKIAQVKP NNTGIRASVY 800
EKTAKNGAKY ADRTFYVTKE RAHGNETYVL LNNTSHNIPL GWFNVKDLNV 850
QNLGKEVKTT QKYTVNKSNN GLSMVPWGTK NQVILTGNNI AQGTFNATKQ 900
VSVGKDVYLY GTINNRTGWV NAKDLTAPTA VKPTTSAAKD YNYTYVIKNG 950
NGYYYVTPNS DTAKYSLKAF NEQPFAVVKE QVINEQTWYY GKLSNGKLAW 1000
IKSTDLAKEL IKYNQTGMTL NQVAQIQAGL QYKPQVQRVP GKWTDANFND 1050
VKHAMDTKRL AQDPALKYQF LRLDQPQNIS IDKINQFLKG KGVLENQGAA 1100
FNKAAQMYGI NEVYLISHAL LETGNGTSQL AKGADVVNNK VVTNSNTKYH 1150
NVFGIAAYDN DPLREGIKYA KQAGWDTVSK AIVGGAKFIG NSYVKAGQNT 1200
LYKMRWNPAH PGTHQYATDI DWANINAKII KGYYDKIGEV GKYFDIPQYK 1250
Length:1,250
Mass (Da):136,868
Last modified:July 19, 2004 - v1
Checksum:iD8C43606B9374D96
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX571857 Genomic DNA. Translation: CAG42763.1.
RefSeqiWP_001074541.1. NC_002953.3.
YP_043113.1. NC_002953.3.

Genome annotation databases

GeneIDi2862752.
KEGGisas:SAS0988.
PATRICi19551413. VBIStaAur96780_1024.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX571857 Genomic DNA. Translation: CAG42763.1 .
RefSeqi WP_001074541.1. NC_002953.3.
YP_043113.1. NC_002953.3.

3D structure databases

ProteinModelPortali Q6GAG0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 282459.SAS0988.

Protein family/group databases

CAZyi GH73. Glycoside Hydrolase Family 73.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2862752.
KEGGi sas:SAS0988.
PATRICi 19551413. VBIStaAur96780_1024.

Phylogenomic databases

eggNOGi COG4193.
HOGENOMi HOG000279968.
KOi K13714.
OMAi KSGWISK.
OrthoDBi EOG6GXTN7.

Family and domain databases

Gene3Di 3.40.80.10. 1 hit.
InterProi IPR002502. Amidase_domain.
IPR013338. Lysozyme_subfam2_dom.
IPR002901. MGlyc_endo_b_GlcNAc_like_dom.
[Graphical view ]
Pfami PF01510. Amidase_2. 1 hit.
PF01832. Glucosaminidase. 1 hit.
[Graphical view ]
SMARTi SM00644. Ami_2. 1 hit.
SM00047. LYZ2. 1 hit.
[Graphical view ]
SUPFAMi SSF55846. SSF55846. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MSSA476.

Entry informationi

Entry nameiATL_STAAS
AccessioniPrimary (citable) accession number: Q6GAG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: July 19, 2004
Last modified: September 3, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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