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Q6GAE8 (PURK_STAAS) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
N5-carboxyaminoimidazole ribonucleotide synthase
Short name=N5-CAIR synthase
EC=6.3.4.18
Alternative name(s):
5-(carboxyamino)imidazole ribonucleotide synthetase
Gene names
Name:purK
Ordered Locus Names:SAS1001
OrganismStaphylococcus aureus (strain MSSA476) [Complete proteome] [HAMAP]
Taxonomic identifier282459 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length374 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO3- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) By similarity.

Catalytic activity

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3- = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 1/2.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the purK/purT family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 374374N5-carboxyaminoimidazole ribonucleotide synthase
PRO_0000075007

Regions

Domain112 – 296185ATP-grasp
Nucleotide binding183 – 1864ATP By similarity
Nucleotide binding266 – 2672ATP By similarity

Sites

Binding site1081ATP By similarity
Binding site1481ATP By similarity
Binding site1591ATP By similarity
Binding site1911ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6GAE8 [UniParc].

Last modified July 19, 2004. Version 1.
Checksum: 1613C944ACFF6616

FASTA37442,466
        10         20         30         40         50         60 
MSFNKLKFGA TIGIIGGGQL GKMMAQSAQK MGYKVVVLDP SEDCPCRYVA HEFIQAKYDD 

        70         80         90        100        110        120 
EKALNQLGQK CDVITYEFEN ISAQQLKLLC EKYNIPQGYQ AIQLLQDRLT EKETLKSAGT 

       130        140        150        160        170        180 
KVVPFISVKE STDIDKAIET LGYPFIVKTR FGGYDGKGQV LINNEKDLQE GFKLIETSEC 

       190        200        210        220        230        240 
VAEKYLNIKK EVSLTVTRGN NNQITFFPLQ ENEHRNQILF KTIVPARIDK TAEAKEQVNK 

       250        260        270        280        290        300 
IIQSIHFIGT FTVEFFIDSN NQLYVNEIAP RPHNSGHYSI EACDYSQFDT HILAVTGQSL 

       310        320        330        340        350        360 
PNSIELLKPA VMMNLLGKDL DLLENEFNEH PEWHLHIYGK SERKDSRKMG HMTVLTNDVN 

       370 
QTEQDMYAKF EGSN

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX571857 Genomic DNA. Translation: CAG42775.1.
RefSeqYP_043125.1. NC_002953.3.

3D structure databases

ProteinModelPortalQ6GAE8.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ6GAE8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBSTAT00000023964; EBSTAP00000023131; EBSTAG00000023963.
GeneID2862668.
GenomeReviewsGene locus SAS1001 in contig BX571857_GR.
KEGGsas:SAS1001.
PATRIC19551439. VBIStaAur96780_1037.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0026.
GeneTreeEBGT00050000023923.
HOGENOMHBG516369.
OMAGRKMGHF.
PhylomeDBQ6GAE8.
ProtClustDBPRK06019.

Enzyme and pathway databases

BioCycSAUR282459:SAS1001-MONOMER.

Family and domain databases

InterProIPR005875. AIR_COase_ATPase-su.
IPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006140. D-isomer_2_OHA_DH_NAD-bd.
IPR013817. Pre-ATP_grasp.
IPR016185. PreATP-grasp-like.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
Gene3DG3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit.
G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KOK01589.
PfamPF02826. 2-Hacid_dh_C. 1 hit.
PF02222. ATP-grasp. 1 hit.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMsTIGR01161. PurK. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePURK_STAAS
AccessionPrimary (citable) accession number: Q6GAE8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: July 19, 2004
Last modified: January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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