Reviewed, UniProtKB/Swiss-Prot Q6GAE1 (PUR3_STAAS)

Names and origin

Protein names

Recommended name:
    Phosphoribosylglycinamide formyltransferase
    EC=2.1.2.2
Alternative name(s):
    5'-phosphoribosylglycinamide transformylase
    GAR transformylase
      Short name=GART

Gene names

Name:	purN
Ordered Locus Names:	SAS1008

Organism

Staphylococcus aureus (strain MSSA476) [Complete proteome] [HAMAP]

Taxonomic identifier

282459 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the GART family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	phosphoribosylglycinamide formyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	Phosphoribosylglycinamide formyltransferase		PRO_0000074949
Regions
	Region	12 – 14	3	5'-phosphoribosylglycinamide binding By similarity
	Region	91 – 94	4	10-formyltetrahydrofolate binding By similarity
Sites
	Active site	110	1	Proton donor By similarity
	Binding site	66	1	10-formyltetrahydrofolate By similarity
	Binding site	108	1	10-formyltetrahydrofolate By similarity
	Binding site	146	1	10-formyltetrahydrofolate; via amide nitrogen By similarity
	Binding site	175	1	5'-phosphoribosylglycinamide By similarity
	Site	146	1	Raises pKa of active site His By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6GAE1-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: D034134258D89AEE	FASTA	188	21,153
	10 20 30 40 50 60 MVKIAIFASG SGSNFENIVE HVESGKLENI EVTALYTDHQ NAFCIDRAKK HDIPVYINEP 70 80 90 100 110 120 KQFDSKAAYE QHLVSLLNED KVEWIILAGY MRLIGPDLLA SFEGKILNIH PSLLPKYKGI 130 140 150 160 170 180 DAIGQAYHSG DTITGSTVHY VDCGMDTGEI IEQRQCDIRP DDSKEQLEEK VKKLEYELYP SVIAKIVK

References

[1]

"Complete genomes of two clinical Staphylococcus aureus strains: evidence for the rapid evolution of virulence and drug resistance." Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J., Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A., Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C., Clark L., Corton C. , Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z., Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N., Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M., Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004) [PubMed: 15213324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX571857 Genomic DNA. Translation: CAG42782.1.
RefSeq	YP_043132.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6GAE1.
Genome annotation databases
GeneID	2863428.
GenomeReviews	Gene locus SAS1008 in contig BX571857_GR.
KEGG	sas:SAS1008.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6GAE1.
OMA	CSVHFVT.
Enzyme and pathway databases
BioCyc	SAUR282459:SAS1008-MON.
Family and domain databases
InterPro	IPR002376. Formyl_transf_N. IPR001555. GART_AS. IPR004607. PurN_trans. [Graphical view]
Gene3D	G3DSA:3.40.50.170. Formyl_transf_N. 1 hit.
Pfam	PF00551. Formyl_trans_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00639. PurN. 1 hit.
PROSITE	PS00373. GART. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR3_STAAS

Accession

Primary (citable) accession number: Q6GAE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	July 19, 2004
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)