Iron-regulated surface determinant protein A precursor - Staphylococcus aureus (strain MSSA476)

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Q6GA85 (ISDA_STAAS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 60. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Iron-regulated surface determinant protein A

Alternative name(s):
Fur-regulated protein A
Staphylococcal transferrin-binding protein A

Gene names

Name:	isdA
Synonyms:	frpA, stbA
Ordered Locus Names:	SAS1064

Organism

Staphylococcus aureus (strain MSSA476) [Complete proteome] [HAMAP]

Taxonomic identifier

282459 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcus ›

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers its hemin to hemin-free IsdC (apo-IsdC) directly probably through the activated holo-IsdA-apo-IsdC complex and driven by the higher affinity of apo-IsdC for the cofactor. The reaction is reversible. Binds transferrin, lactoferrin, heme, hemoglobin, hemin, fetuin, asialofetuin and protein A. Also binds fibronectin and chains B-beta and gamma of fibrinogen. Could play a role in the removal of heme from hemoglobin. The IsdA-mediated iron-acquisition system from transferrin could play only an ancillary role in the iron uptake whereas the siderophore-mediated iron-acquisition system from transferrin seems to play an essential or dominant role. May function as a reservoir for heme. Involved in adherence of S.aureus to human desquamated nasal epithelial cells and is required for nasal colonization. Protects S.aureus against the bactericidal protease activity of apolactoferrin in vitro and confers resistance to bovine lactoferricin. Also IsdA and/or IsdB promote resistance to hydrogen peroxide and killing by neutrophils By similarity.
Subunit structure	Monomer. Interacts with IsdC By similarity.
Subcellular location	Secreted › cell wall; Peptidoglycan-anchor Probable.
Induction	Repressed by fur in the presence of iron By similarity.
Domain	The NEAT domain is responsible for binding Fe³⁺ and Fe²⁺ heme and fibrinogen. The NEAT domain is an inhibitor of apolactoferrin activity, while the C-domain confers resistance to bovine lactoferricin By similarity.
Sequence similarities	Contains 1 NEAT domain.

Ontologies

Keywords
Cellular component	Cell wall Secreted
Domain	Signal
Ligand	Heme Iron Metal-binding
PTM	Peptidoglycan-anchor
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 46

By similarity

Chain

47 – 316

270

Iron-regulated surface determinant protein A

PRO_0000046092

Propeptide

317 – 350

Removed by sortase A Potential

PRO_0000046093

Regions

Domain

62 – 184

123

NEAT

Motif

313 – 317

LPXTG sorting signal Potential

Sites

Metal binding

166

Iron (heme axial ligand) By similarity

Binding site

Heme By similarity

Binding site

Heme By similarity

Amino acid modifications

Modified residue

316

Pentaglycyl murein peptidoglycan amidated threonine Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q6GA85 [UniParc].

Last modified July 19, 2004. Version 1.
Checksum: 14882D25C0EA3CA6
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FASTA

350

38,746

        10         20         30         40         50         60 
MTKHYLNSKY QSEQRSSAMK KITMGTASII LGSLVYIGAD SQQVNAATEA TNATNNQSTQ 

        70         80         90        100        110        120 
VSQATSQPIN FQVQKDGSSE KSHMDDYMQH PGKVIKQNNK YYFQTVLNNA SFWKEYKFYN 

       130        140        150        160        170        180 
ANNQELATTV VNDNKKADTR TINVAVEPGY KSLTTKVHIV VPQINYNHRY TTHLEFEKAI 

       190        200        210        220        230        240 
PTLADAAKPN NVKPVQPKPA QPKTPTEQTK PVQPKVEKVK PTVTTTSKVE DNHSTKVVST 

       250        260        270        280        290        300 
DTTKDQTKTQ TAHTVKTAQT AQEQNKVQTP VKDVATAKSE SNNQAVSDNK SQQTNKVTKH 

       310        320        330        340        350 
NETPKQASKA KELPKTGLTS VDNFISTVAF ATLALLGSLS LLLFKRKESK

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References

[1]

"Complete genomes of two clinical Staphylococcus aureus strains: evidence for the rapid evolution of virulence and drug resistance."
Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J., Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A., Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C., Clark L., Corton C.

Parkhill J.
Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MSSA476.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571857 Genomic DNA. Translation: CAG42838.1.
RefSeq	YP_043188.1. NC_002953.3.
3D structure databases
ProteinModelPortal	Q6GA85.
SMR	Q6GA85. Positions 63-184.
ModBase	Search...
Protein-protein interaction databases
STRING	282459.SAS1064.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2864366.
KEGG	sas:SAS1064.
PATRIC	19551571. VBIStaAur96780_1103.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG5386.
HOGENOM	HOG000107381.
KO	K14193.
OMA	TTVVNDD.
ProtClustDB	CLSK885153.
Family and domain databases
InterPro	IPR019948. Gram-positive_anchor. IPR019931. LPXTG-motif_cell_wall_anchor. IPR006635. NEA_transpt. [Graphical view]
Pfam	PF00746. Gram_pos_anchor. 1 hit. PF05031. NEAT. 1 hit. [Graphical view]
SMART	SM00725. NEAT. 1 hit. [Graphical view]
TIGRFAMs	TIGR01167. LPXTG_anchor. 1 hit.
PROSITE	PS50847. GRAM_POS_ANCHORING. 1 hit. PS50978. NEAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISDA_STAAS

Accession

Primary (citable) accession number: Q6GA85

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	July 19, 2004
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents