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Q6GA85

- ISDA_STAAS

UniProt

Q6GA85 - ISDA_STAAS

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Protein

Iron-regulated surface determinant protein A

Gene

isdA

Organism
Staphylococcus aureus (strain MSSA476)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Transfers its hemin to hemin-free IsdC (apo-IsdC) directly probably through the activated holo-IsdA-apo-IsdC complex and driven by the higher affinity of apo-IsdC for the cofactor. The reaction is reversible. Binds transferrin, lactoferrin, heme, hemoglobin, hemin, fetuin, asialofetuin and protein A. Also binds fibronectin and chains B-beta and gamma of fibrinogen. Could play a role in the removal of heme from hemoglobin. The IsdA-mediated iron-acquisition system from transferrin could play only an ancillary role in the iron uptake whereas the siderophore-mediated iron-acquisition system from transferrin seems to play an essential or dominant role. May function as a reservoir for heme. Involved in adherence of S.aureus to human desquamated nasal epithelial cells and is required for nasal colonization. Protects S.aureus against the bactericidal protease activity of apolactoferrin in vitro and confers resistance to bovine lactoferricin. Also IsdA and/or IsdB promote resistance to hydrogen peroxide and killing by neutrophils (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei75 – 751HemeBy similarity
Binding sitei82 – 821HemeBy similarity
Metal bindingi166 – 1661Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Iron-regulated surface determinant protein A
Alternative name(s):
Fur-regulated protein A
Staphylococcal transferrin-binding protein A
Gene namesi
Name:isdA
Synonyms:frpA, stbA
Ordered Locus Names:SAS1064
OrganismiStaphylococcus aureus (strain MSSA476)
Taxonomic identifieri282459 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000002201: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cell wall Source: UniProtKB-KW
  2. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4646By similarityAdd
BLAST
Chaini47 – 316270Iron-regulated surface determinant protein APRO_0000046092Add
BLAST
Propeptidei317 – 35034Removed by sortase APROSITE-ProRule annotationPRO_0000046093Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei316 – 3161Pentaglycyl murein peptidoglycan amidated threoninePROSITE-ProRule annotation

Keywords - PTMi

Peptidoglycan-anchor

Expressioni

Inductioni

Repressed by fur in the presence of iron.By similarity

Interactioni

Subunit structurei

Monomer. Interacts with IsdC (By similarity).By similarity

Protein-protein interaction databases

STRINGi282459.SAS1064.

Structurei

3D structure databases

ProteinModelPortaliQ6GA85.
SMRiQ6GA85. Positions 63-184.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini62 – 184123NEATPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi313 – 3175LPXTG sorting signalPROSITE-ProRule annotation

Domaini

The NEAT domain is responsible for binding Fe3+ and Fe2+ heme and fibrinogen. The NEAT domain is an inhibitor of apolactoferrin activity, while the C-domain confers resistance to bovine lactoferricin (By similarity).By similarity

Sequence similaritiesi

Contains 1 NEAT domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5386.
HOGENOMiHOG000107381.
KOiK14193.
OMAiVNDDKKA.
OrthoDBiEOG68SVXG.

Family and domain databases

InterProiIPR019948. Gram-positive_anchor.
IPR019931. LPXTG_anchor.
IPR006635. NEAT_dom.
[Graphical view]
PfamiPF00746. Gram_pos_anchor. 1 hit.
PF05031. NEAT. 1 hit.
[Graphical view]
SMARTiSM00725. NEAT. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01167. LPXTG_anchor. 1 hit.
PROSITEiPS50847. GRAM_POS_ANCHORING. 1 hit.
PS50978. NEAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6GA85-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTKHYLNSKY QSEQRSSAMK KITMGTASII LGSLVYIGAD SQQVNAATEA
60 70 80 90 100
TNATNNQSTQ VSQATSQPIN FQVQKDGSSE KSHMDDYMQH PGKVIKQNNK
110 120 130 140 150
YYFQTVLNNA SFWKEYKFYN ANNQELATTV VNDNKKADTR TINVAVEPGY
160 170 180 190 200
KSLTTKVHIV VPQINYNHRY TTHLEFEKAI PTLADAAKPN NVKPVQPKPA
210 220 230 240 250
QPKTPTEQTK PVQPKVEKVK PTVTTTSKVE DNHSTKVVST DTTKDQTKTQ
260 270 280 290 300
TAHTVKTAQT AQEQNKVQTP VKDVATAKSE SNNQAVSDNK SQQTNKVTKH
310 320 330 340 350
NETPKQASKA KELPKTGLTS VDNFISTVAF ATLALLGSLS LLLFKRKESK
Length:350
Mass (Da):38,746
Last modified:July 19, 2004 - v1
Checksum:i14882D25C0EA3CA6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX571857 Genomic DNA. Translation: CAG42838.1.
RefSeqiYP_043188.1. NC_002953.3.

Genome annotation databases

GeneIDi2864366.
KEGGisas:SAS1064.
PATRICi19551571. VBIStaAur96780_1103.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX571857 Genomic DNA. Translation: CAG42838.1 .
RefSeqi YP_043188.1. NC_002953.3.

3D structure databases

ProteinModelPortali Q6GA85.
SMRi Q6GA85. Positions 63-184.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 282459.SAS1064.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2864366.
KEGGi sas:SAS1064.
PATRICi 19551571. VBIStaAur96780_1103.

Phylogenomic databases

eggNOGi COG5386.
HOGENOMi HOG000107381.
KOi K14193.
OMAi VNDDKKA.
OrthoDBi EOG68SVXG.

Miscellaneous databases

PROi Q6GA85.

Family and domain databases

InterProi IPR019948. Gram-positive_anchor.
IPR019931. LPXTG_anchor.
IPR006635. NEAT_dom.
[Graphical view ]
Pfami PF00746. Gram_pos_anchor. 1 hit.
PF05031. NEAT. 1 hit.
[Graphical view ]
SMARTi SM00725. NEAT. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01167. LPXTG_anchor. 1 hit.
PROSITEi PS50847. GRAM_POS_ANCHORING. 1 hit.
PS50978. NEAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MSSA476.

Entry informationi

Entry nameiISDA_STAAS
AccessioniPrimary (citable) accession number: Q6GA85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: July 19, 2004
Last modified: October 29, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3