Q6GA79 (ISDG_STAAS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heme-degrading monooxygenase isdG
EC=1.14.99.3

Alternative name(s):
Heme oxygenase
Iron-regulated surface determinant isdG
Iron-responsive surface determinant isdG

Gene names

Name:	isdG
Ordered Locus Names:	SAS1070

Organism

Staphylococcus aureus (strain MSSA476) [Complete proteome] [HAMAP]

Taxonomic identifier

282459 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	107 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows bacterial pathogens to use the host heme as an iron source. Catalyzes the oxidative degradation of the heme macrocyclic porphyrin ring in the presence of a suitable electron donor such as ascorbate or NADPH--cytochrome P450 reductase, with subsequent release of free iron By similarity. HAMAP MF_01272
Catalytic activity	Heme + 3 AH2 + 3 O2 = biliverdin + Fe2+ + CO + 3 A + 3 H2O. HAMAP MF_01272
Subunit structure	Homodimer By similarity. HAMAP MF_01272
Subcellular location	Cytoplasm By similarity HAMAP MF_01272.
Sequence similarities	Belongs to the antibiotic biosynthesis monooxygenase family. Heme-degrading monooxygenase isdG subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	iron assimilation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	heme oxygenase (decyclizing) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 107	107	Heme-degrading monooxygenase isdG HAMAP MF_01272		PRO_0000270085
Sites
	Metal binding	7	1	Iron Potential
	Metal binding	77	1	Iron (heme axial ligand) Potential
	Site	67	1	Transition state stabilizer Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6GA79 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: DB13A134D5EC4FF0	FASTA	107	12,546
	10 20 30 40 50 60 MKFMAENRLT LTKGTAKDII ERFYTRHGIE TLEGFDGMFV TQTLEQEDFD EVKILTVWKS 70 80 90 100 KQAFTDWLKS DVFKAAHKHV RSKNEDESSP IINNKVITYD IGYSYMK

References

[1]

"Complete genomes of two clinical Staphylococcus aureus strains: evidence for the rapid evolution of virulence and drug resistance." Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J., Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A., Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C., Clark L., Corton C. , Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z., Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N., Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M., Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004) [PubMed: 15213324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MSSA476.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571857 Genomic DNA. Translation: CAG42844.1.
RefSeq	YP_043194.1. NC_002953.3.
3D structure databases
ProteinModelPortal	Q6GA79.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6GA79.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000022649; EBSTAP00000021816; EBSTAG00000022648.
GeneID	2862706.
GenomeReviews	Gene locus SAS1070 in contig BX571857_GR.
KEGG	sas:SAS1070.
PATRIC	19551583. VBIStaAur96780_1109.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2329.
GeneTree	EBGT00050000024362.
HOGENOM	HBG616589.
OMA	GEGERIM.
ProtClustDB	PRK13312.
Enzyme and pathway databases
BioCyc	SAUR282459:SAS1070-MONOMER.
Family and domain databases
HAMAP	MF_01272. IsdG. [Tree]
InterPro	IPR007138. Antibiotic_mOase. IPR011008. Dimeric_a/b-barrel. IPR023953. Heme-degrad_mOase. [Graphical view]
KO	K00510.
Pfam	PF03992. ABM. 1 hit. [Graphical view]
SUPFAM	SSF54909. Dimer_A_B_barrel. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISDG_STAAS

Accession

Primary (citable) accession number: Q6GA79

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	July 19, 2004
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program