Q6GA45 (OTC_STAAS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ornithine carbamoyltransferase
Short name=OTCase
EC=2.1.3.3

Gene names

Name:	argF
Ordered Locus Names:	SAS1102

Organism

Staphylococcus aureus (strain MSSA476) [Complete proteome] [HAMAP]

Taxonomic identifier

282459 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline. HAMAP MF_01109
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3. HAMAP MF_01109
Subcellular location	Cytoplasm Probable HAMAP MF_01109.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ornithine carbamoyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro ornithine carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Ornithine carbamoyltransferase HAMAP MF_01109		PRO_0000113017
Regions
	Region	56 – 60	5	Carbamoyl phosphate binding By similarity
	Region	272 – 275	4	Ornithine binding By similarity
Sites
	Binding site	107	1	Carbamoyl phosphate By similarity
	Binding site	134	1	Carbamoyl phosphate By similarity
	Site	31	1	Important for structural integrity By similarity
	Site	147	1	Important for structural integrity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6GA45 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 4AEBB45E867C04A1	FASTA	333	37,534
	10 20 30 40 50 60 MKNLRNRSFL TLLDFSRQEV EFLLTLSEDL KRAKYIGTEK PMLKNKNIAL LFEKDSTRTR 70 80 90 100 110 120 CAFEVAAHDQ GANVTYLGPT GSQMGKKETT KDTARVLGGM YDGIEYRGFS QRTVETLAEY 130 140 150 160 170 180 SGVPVWNGLT DEDHPTQVLA DFLTAKEVLK KDYADINFTY VGDGRNNVAN ALMQGAAIMG 190 200 210 220 230 240 MNFHLVCPKE LNPTDELLNR CKNIAAENGG NILITDNIDQ GVKGSDVIYT DVWVSMGEPD 250 260 270 280 290 300 EVWKERLELL KPYQVNKEMM DKTGNPNVIF EHCLPSFHNA DTKIGQQIFE KYGIREMEVT 310 320 330 DEVFESKASV VFQEAENRMH TIKAVMVATL GEF

References

[1]

"Complete genomes of two clinical Staphylococcus aureus strains: evidence for the rapid evolution of virulence and drug resistance." Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J., Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A., Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C., Clark L., Corton C. , Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z., Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N., Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M., Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004) [PubMed: 15213324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MSSA476.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571857 Genomic DNA. Translation: CAG42878.1.
RefSeq	YP_043228.1. NC_002953.3.
3D structure databases
ProteinModelPortal	Q6GA45.
SMR	Q6GA45. Positions 2-333.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6GA45.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000023723; EBSTAP00000022890; EBSTAG00000023722.
GeneID	2863211.
GenomeReviews	Gene locus SAS1102 in contig BX571857_GR.
KEGG	sas:SAS1102.
PATRIC	19551655. VBIStaAur96780_1146.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0078.
GeneTree	EBGT00050000023906.
HOGENOM	HBG579429.
OMA	DFRIFAP.
ProtClustDB	PRK04284.
Enzyme and pathway databases
BioCyc	SAUR282459:SAS1102-MONOMER.
Family and domain databases
HAMAP	MF_01109. OTCase. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002292. Orn/put_carbamltrans. IPR024904. Orn_carbamltrans. [Graphical view]
KO	K00611.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00102. OTCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00658. Orni_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTC_STAAS

Accession

Primary (citable) accession number: Q6GA45

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	July 19, 2004
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program